TY - JOUR
T1 - Diverse Partitiviruses From the Phytopathogenic Fungus, Rosellinia necatrix
AU - Telengech, Paul
AU - Hisano, Sakae
AU - Mugambi, Cyrus
AU - Hyodo, Kiwamu
AU - Arjona-López, Juan Manuel
AU - López-Herrera, Carlos José
AU - Kanematsu, Satoko
AU - Kondo, Hideki
AU - Suzuki, Nobuhiro
N1 - Funding Information:
The authors sincerely thank Dr. Donald L. Nuss (IBBR, University of Maryland) and Dr. Masanobu Tabata (Forestry & Forest Products Research Institute) for their generous gift of the C. parasitica strains EP155, ?dcl2, and ?agl2 and some R. necatrix field isolates used in this study. Funding. This work was supported in part by Yomogi Inc. (to NS), the Program for the Promotion of Basic and Applied Research for Innovation in Bio-Oriented Industries (to SK and NS), and Grants-in-Aid for Scientific Research (A) and on Innovative Areas from the Japanese Ministry of Education, Culture, Sports, Science, and Technology (MEXT) (17H01463 and 16H06436, 16H06429, and 16K21723 to NS). The funder, Yomogi Inc., was not involved in the study design, collection, analysis, interpretation of data, the writing of this article or the decision to submit it for publication.
Publisher Copyright:
© Copyright © 2020 Telengech, Hisano, Mugambi, Hyodo, Arjona-López, López-Herrera, Kanematsu, Kondo and Suzuki.
PY - 2020/6/26
Y1 - 2020/6/26
N2 - Partitiviruses (dsRNA viruses, family Partitiviridae) are ubiquitously detected in plants and fungi. Although previous surveys suggested their omnipresence in the white root rot fungus, Rosellinia necatrix, only a few of them have been molecularly and biologically characterized thus far. We report the characterization of a total of 20 partitiviruses from 16 R. necatrix strains belonging to 15 new species, for which “Rosellinia necatrix partitivirus 11–Rosellinia necatrix partitivirus 25” were proposed, and 5 previously reported species. The newly identified partitiviruses have been taxonomically placed in two genera, Alphapartitivirus, and Betapartitivirus. Some partitiviruses were transfected into reference strains of the natural host, R. necatrix, and an experimental host, Cryphonectria parasitica, using purified virions. A comparative analysis of resultant transfectants revealed interesting differences and similarities between the RNA accumulation and symptom induction patterns of R. necatrix and C. parasitica. Other interesting findings include the identification of a probable reassortment event and a quintuple partitivirus infection of a single fungal strain. These combined results provide a foundation for further studies aimed at elucidating mechanisms that underly the differences observed.
AB - Partitiviruses (dsRNA viruses, family Partitiviridae) are ubiquitously detected in plants and fungi. Although previous surveys suggested their omnipresence in the white root rot fungus, Rosellinia necatrix, only a few of them have been molecularly and biologically characterized thus far. We report the characterization of a total of 20 partitiviruses from 16 R. necatrix strains belonging to 15 new species, for which “Rosellinia necatrix partitivirus 11–Rosellinia necatrix partitivirus 25” were proposed, and 5 previously reported species. The newly identified partitiviruses have been taxonomically placed in two genera, Alphapartitivirus, and Betapartitivirus. Some partitiviruses were transfected into reference strains of the natural host, R. necatrix, and an experimental host, Cryphonectria parasitica, using purified virions. A comparative analysis of resultant transfectants revealed interesting differences and similarities between the RNA accumulation and symptom induction patterns of R. necatrix and C. parasitica. Other interesting findings include the identification of a probable reassortment event and a quintuple partitivirus infection of a single fungal strain. These combined results provide a foundation for further studies aimed at elucidating mechanisms that underly the differences observed.
KW - Cryphonectria parasitica
KW - Rosellinia necatrix
KW - diversity
KW - dsRNA virus
KW - horizontal transfer
KW - partitivirus
KW - phytopathogenic fungus
KW - reassortment
UR - http://www.scopus.com/inward/record.url?scp=85087738181&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85087738181&partnerID=8YFLogxK
U2 - 10.3389/fmicb.2020.01064
DO - 10.3389/fmicb.2020.01064
M3 - Article
AN - SCOPUS:85087738181
VL - 11
JO - Frontiers in Microbiology
JF - Frontiers in Microbiology
SN - 1664-302X
M1 - 1064
ER -