Diverse effects of cyclosporine on hepatitis C virus strain replication

Naoto Ishii, Koichi Watashi, Takayuki Hishiki, Kaku Goto, Daisuke Inoue, Makoto Hijikata, Takaji Wakita, Nobuyuki Kato, Kunitada Shimotohno

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Abstract

Recently, a production system for infectious particles of hepatitis C virus (HCV) utilizing the genotype 2a JFH1 strain has been developed. This strain has a high capacity for replication in the cells. Cyclosporine (CsA) has a suppressive effect on HCV replication. In this report, we characterize the anti-HCV effect of CsA. We observe that the presence of viral structural proteins does not influence the anti-HCV activity of CsA. Among HCV strains, the replication of genotype 1b replicons was strongly suppressed by treatment with CsA. In contrast, JFH1 replication was less sensitive to CsA and its analog, NIM811. Replication of JFH1 did not require the cellular replication cofactor, cyclophilin B (CyPB). CyPB stimulated the RNA binding activity of NS5B in the genotype 1b replicon but not the genotype 2a JFH1 strain. These findings provide an insight into the mechanisms of diversity governing virus-cell interactions and in the sensitivity of these strains to antiviral agents.

Original languageEnglish
Pages (from-to)4510-4520
Number of pages11
JournalJournal of Virology
Volume80
Issue number9
DOIs
Publication statusPublished - May 2006

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ASJC Scopus subject areas

  • Immunology

Cite this

Ishii, N., Watashi, K., Hishiki, T., Goto, K., Inoue, D., Hijikata, M., Wakita, T., Kato, N., & Shimotohno, K. (2006). Diverse effects of cyclosporine on hepatitis C virus strain replication. Journal of Virology, 80(9), 4510-4520. https://doi.org/10.1128/JVI.80.9.4510-4520.2006