TY - JOUR
T1 - Diverse effects of cyclosporine on hepatitis C virus strain replication
AU - Ishii, Naoto
AU - Watashi, Koichi
AU - Hishiki, Takayuki
AU - Goto, Kaku
AU - Inoue, Daisuke
AU - Hijikata, Makoto
AU - Wakita, Takaji
AU - Kato, Nobuyuki
AU - Shimotohno, Kunitada
PY - 2006/5
Y1 - 2006/5
N2 - Recently, a production system for infectious particles of hepatitis C virus (HCV) utilizing the genotype 2a JFH1 strain has been developed. This strain has a high capacity for replication in the cells. Cyclosporine (CsA) has a suppressive effect on HCV replication. In this report, we characterize the anti-HCV effect of CsA. We observe that the presence of viral structural proteins does not influence the anti-HCV activity of CsA. Among HCV strains, the replication of genotype 1b replicons was strongly suppressed by treatment with CsA. In contrast, JFH1 replication was less sensitive to CsA and its analog, NIM811. Replication of JFH1 did not require the cellular replication cofactor, cyclophilin B (CyPB). CyPB stimulated the RNA binding activity of NS5B in the genotype 1b replicon but not the genotype 2a JFH1 strain. These findings provide an insight into the mechanisms of diversity governing virus-cell interactions and in the sensitivity of these strains to antiviral agents.
AB - Recently, a production system for infectious particles of hepatitis C virus (HCV) utilizing the genotype 2a JFH1 strain has been developed. This strain has a high capacity for replication in the cells. Cyclosporine (CsA) has a suppressive effect on HCV replication. In this report, we characterize the anti-HCV effect of CsA. We observe that the presence of viral structural proteins does not influence the anti-HCV activity of CsA. Among HCV strains, the replication of genotype 1b replicons was strongly suppressed by treatment with CsA. In contrast, JFH1 replication was less sensitive to CsA and its analog, NIM811. Replication of JFH1 did not require the cellular replication cofactor, cyclophilin B (CyPB). CyPB stimulated the RNA binding activity of NS5B in the genotype 1b replicon but not the genotype 2a JFH1 strain. These findings provide an insight into the mechanisms of diversity governing virus-cell interactions and in the sensitivity of these strains to antiviral agents.
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U2 - 10.1128/JVI.80.9.4510-4520.2006
DO - 10.1128/JVI.80.9.4510-4520.2006
M3 - Article
C2 - 16611911
AN - SCOPUS:33646194456
SN - 0022-538X
VL - 80
SP - 4510
EP - 4520
JO - Journal of Virology
JF - Journal of Virology
IS - 9
ER -
