Divergent evolution of wild and cultivated subspecies of Triticum timopheevii as revealed by the study of PolA1 gene

Hiroko Takahashi, Bhuwan Rai, Kenji Kato, Ikuo Nakamura

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Triticum timopheevii (genome symbol AAGG) comprises two subspecies, cultivated ssp. timopheevii, and wild ssp. armeniacum. These two subspecies are considered as allotetraploids of AA genome from Triticum diploid species and SS genome from Aegilops species. The difference in genome symbol (G vs. S) is due to wide genetic variations among four SS genome species, Ae. bicornis, Ae. longissima, Ae. searsii, and Ae. speltoides. In order to study the origin of T. timopheevii, we compared 19th intron (PI19) sequence of the PolA1 gene, encoding the largest subunit of RNA polymerase I. Two different sized DNA fragments containing PI19 sequences (PI19A and PI19G) were amplified both in ssp. timopheevii and ssp. armeniacum. Shorter PI19A (112 bp) sequences of both subspecies were identical to PI19 sequences of two AA species, T. monococcum and T. urartu. Interestingly, the longer PI19G (241-243 bp) sequences of ssp. armeniacum showed more similarity to PI19 sequences of Ae. speltoides whereas ssp. timopheevii showed more similarity to PI19 sequences of other three SS genome species. The results indicated that two subspecies of T. timopheevii, ssp. armeniacum or ssp. timopheevii, might have arisen independently by allotetraploidization of AA genome with Ae. speltoides or one of the remaining three Aegilops species, respectively.

Original languageEnglish
Pages (from-to)101-109
Number of pages9
JournalGenetic Resources and Crop Evolution
Volume57
Issue number1
DOIs
Publication statusPublished - Jan 2010

Fingerprint

Triticum timopheevii
divergent evolution
subspecies
Triticum
genome
Genome
gene
Genes
genes
Aegilops
RNA Polymerase I
DNA-directed RNA polymerase
Diploidy
Introns
genetic variation
RNA
introns
diploidy
nucleotide sequences
DNA

Keywords

  • Allotetraploid
  • Evolution
  • PolA1 gene
  • PSID
  • Triticum timopheevii

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Genetics
  • Plant Science
  • Ecology, Evolution, Behavior and Systematics

Cite this

Divergent evolution of wild and cultivated subspecies of Triticum timopheevii as revealed by the study of PolA1 gene. / Takahashi, Hiroko; Rai, Bhuwan; Kato, Kenji; Nakamura, Ikuo.

In: Genetic Resources and Crop Evolution, Vol. 57, No. 1, 01.2010, p. 101-109.

Research output: Contribution to journalArticle

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