Diuretics Prime Plant Immunity in Arabidopsis thaliana

Yoshiteru Noutoshi, Mika Ikeda, Ken Shirasu

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Plant activators are agrochemicals that activate the plant immune system, thereby enhancing disease resistance. Due to their prophylactic and durable effects on a wide spectrum of diseases, plant activators can provide synergistic crop protection when used in combination with traditional pest controls. Although plant activators have achieved great success in wet-rice farming practices in Asia, their use is still limited. To isolate novel plant activators applicable to other crops, we screened a chemical library using a method that can selectively identify immune-priming compounds. Here, we report the isolation and characterization of three diuretics, bumetanide, bendroflumethiazide and clopamide, as immune-priming compounds. These drugs upregulate the immunity-related cell death of Arabidopsis suspension-cultured cells induced with an avirulent strain of Pseudomonas syringae pv. tomato in a concentration-dependent manner. The application of these compounds to Arabidopsis plants confers disease resistance to not only the avirulent but also a virulent strain of the pathogen. Unlike salicylic acid, an endogenous phytohormone that governs disease resistance in response to biotrophic pathogens, the three diuretic compounds analyzed here do not induce PR1 or inhibit plant growth, showing potential as lead compounds in a practical application.

Original languageEnglish
Article numbere48443
JournalPLoS One
Volume7
Issue number10
DOIs
Publication statusPublished - Oct 29 2012

Fingerprint

Plant Immunity
diuretics
Diuretics
Arabidopsis
Disease Resistance
Arabidopsis thaliana
immunity
disease resistance
Plant Diseases
Pathogens
plant diseases and disorders
Crops
Clopamide
Bendroflumethiazide
Pest control
Small Molecule Libraries
Agrochemicals
Lead compounds
Pseudomonas syringae
Bumetanide

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Diuretics Prime Plant Immunity in Arabidopsis thaliana. / Noutoshi, Yoshiteru; Ikeda, Mika; Shirasu, Ken.

In: PLoS One, Vol. 7, No. 10, e48443, 29.10.2012.

Research output: Contribution to journalArticle

Noutoshi, Yoshiteru ; Ikeda, Mika ; Shirasu, Ken. / Diuretics Prime Plant Immunity in Arabidopsis thaliana. In: PLoS One. 2012 ; Vol. 7, No. 10.
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