Distribution of soluble N-ethylmaleimide fusion protein attachment proteins (SNAPS) in the rat nervous system

Tei-ichi Nishiki, I. Nihonmatsu, Y. Tsuhara, M. Kawasaki, M. Sekiguchi, K. Sato, A. Mizoguchi, M. Takahashi

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Soluble N-ethylmaleimide-sensitive fusion protein (NSF) attachment protein (SNAP) plays an essential role in vesicular transport and the release of neurotransmitters and hormones through associations with NSF and SNAP receptors (SNAREs). Three isoforms (α, β and γ) of SNAP are expressed in mammals. We have generated isoform-specific antibodies and studied the expression and distribution of these SNAP isoforms in the rat nervous system. Each antibody specifically recognized α-, β- or γ-SNAP in an isoform-specific manner in immunoblots of brain homogenate. α- And γ-SNAP were ubiquitously expressed in various tissues, whereas β-SNAP was expressed only in brain. After subcellular fractionation of brain homogenates, all three isoforms were recovered in both soluble and particulate fractions. Immunohistochemistry revealed that α- and β-SNAP were generally differentially distributed both in synaptic and non-synaptic regions, including brain white matter. The presynaptic location of both α- and β-SNAP was confirmed by immunoelectron microscopy. At the neuromuscular junction, immunoreactive α-SNAP was identified in synaptic vesicles, while in the cerebellum, β-SNAP was present in the presynaptic membranes of basket neuron and mossy fiber terminals. From these results we suggest that both α- and β-SNAP may play an important role in neurotransmitter release as well as in constitutive vesicular transport.

Original languageEnglish
Pages (from-to)363-371
Number of pages9
JournalNeuroscience
Volume107
Issue number3
DOIs
Publication statusPublished - Nov 23 2001
Externally publishedYes

Fingerprint

Ethylmaleimide
Nervous System
Protein Isoforms
Brain
Proteins
Neurotransmitter Agents
Soluble N-Ethylmaleimide-Sensitive Factor Attachment Proteins
N-Ethylmaleimide-Sensitive Proteins
SNARE Proteins
Immunoelectron Microscopy
Antibodies
Synaptic Vesicles
Neuromuscular Junction
Cerebellum
Mammals
Immunohistochemistry
Hormones
Neurons
Membranes

Keywords

  • Exocytosis
  • Neurotransmitter release
  • NSF
  • SNARE
  • Synaptic transmission

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Nishiki, T., Nihonmatsu, I., Tsuhara, Y., Kawasaki, M., Sekiguchi, M., Sato, K., ... Takahashi, M. (2001). Distribution of soluble N-ethylmaleimide fusion protein attachment proteins (SNAPS) in the rat nervous system. Neuroscience, 107(3), 363-371. https://doi.org/10.1016/S0306-4522(01)00370-0

Distribution of soluble N-ethylmaleimide fusion protein attachment proteins (SNAPS) in the rat nervous system. / Nishiki, Tei-ichi; Nihonmatsu, I.; Tsuhara, Y.; Kawasaki, M.; Sekiguchi, M.; Sato, K.; Mizoguchi, A.; Takahashi, M.

In: Neuroscience, Vol. 107, No. 3, 23.11.2001, p. 363-371.

Research output: Contribution to journalArticle

Nishiki, T, Nihonmatsu, I, Tsuhara, Y, Kawasaki, M, Sekiguchi, M, Sato, K, Mizoguchi, A & Takahashi, M 2001, 'Distribution of soluble N-ethylmaleimide fusion protein attachment proteins (SNAPS) in the rat nervous system', Neuroscience, vol. 107, no. 3, pp. 363-371. https://doi.org/10.1016/S0306-4522(01)00370-0
Nishiki, Tei-ichi ; Nihonmatsu, I. ; Tsuhara, Y. ; Kawasaki, M. ; Sekiguchi, M. ; Sato, K. ; Mizoguchi, A. ; Takahashi, M. / Distribution of soluble N-ethylmaleimide fusion protein attachment proteins (SNAPS) in the rat nervous system. In: Neuroscience. 2001 ; Vol. 107, No. 3. pp. 363-371.
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