Distribution of Porphyromonas gingivalis fimA genotypes in cardiovascular specimens from Japanese patients

K. Nakano, Hiroaki Inaba, R. Nomura, H. Nemoto, H. Takeuchi, H. Yoshioka, K. Toda, K. Taniguchi, A. Amano, T. Ooshima

Research output: Contribution to journalArticle

51 Citations (Scopus)

Abstract

Introduction: Porphyromonas gingivalis, a major periodontal pathogen, is gaining increasing attention for its possible association with cardiovascular diseases. Its fimbriae are classified into six genotypes (types I-V and Ib) based on the diversity of the fimA genes encoding the fimbrial subunits. In this study, fimA genotypic distribution was analyzed in P. gingivalis-infected cardiovascular specimens. Methods: A total of 112 heart valves and 80 atheromatous plaque specimens were collected from patients undergoing cardiovascular surgery, as well as 56 dental plaque specimens. Bacterial DNA was extracted from each, and polymerase chain reaction analysis was carried out with a P. gingivalis-specific set of primers. P. gingivalis-positive specimens were further analyzed to discriminate the fimA genotype using polymerase chain reaction with fimA type-specific primer sets. Results: P. gingivalis was detected in 10.4% of the cardiovascular specimens and 50.0% of the dental plaque samples. In the latter, type II was most frequently detected (35.7%), followed by types I (28.6%) and IV (21.4%), while types IV and II were detected with considerable frequencies of 45.0% and 30.0%, respectively, in the cardiovascular specimens. In contrast, the occurrence of type I was limited (5.0%) in the cardiovascular specimens. Conclusion: These results suggest that specific fimA genotypic clones, which are reportedly associated with periodontitis, are also frequently harbored in cardiovascular specimens, indicating the possible involvement of type II and IV clones in the initiation and progression of cardiovascular diseases.

Original languageEnglish
Pages (from-to)170-172
Number of pages3
JournalOral Microbiology and Immunology
Volume23
Issue number2
DOIs
Publication statusPublished - Apr 2008
Externally publishedYes

Fingerprint

Porphyromonas gingivalis
Genotype
Dental Plaque
Cardiovascular Diseases
Clone Cells
Bacterial DNA
Polymerase Chain Reaction
Periodontitis
Heart Valves
Atherosclerotic Plaques
Genes

Keywords

  • Cardiovascular specimens
  • FimA genotype
  • Polymerase chain reaction
  • Porphyromonas gingivalis

ASJC Scopus subject areas

  • Immunology
  • Microbiology (medical)
  • Dentistry(all)

Cite this

Distribution of Porphyromonas gingivalis fimA genotypes in cardiovascular specimens from Japanese patients. / Nakano, K.; Inaba, Hiroaki; Nomura, R.; Nemoto, H.; Takeuchi, H.; Yoshioka, H.; Toda, K.; Taniguchi, K.; Amano, A.; Ooshima, T.

In: Oral Microbiology and Immunology, Vol. 23, No. 2, 04.2008, p. 170-172.

Research output: Contribution to journalArticle

Nakano, K, Inaba, H, Nomura, R, Nemoto, H, Takeuchi, H, Yoshioka, H, Toda, K, Taniguchi, K, Amano, A & Ooshima, T 2008, 'Distribution of Porphyromonas gingivalis fimA genotypes in cardiovascular specimens from Japanese patients', Oral Microbiology and Immunology, vol. 23, no. 2, pp. 170-172. https://doi.org/10.1111/j.1399-302X.2007.00406.x
Nakano, K. ; Inaba, Hiroaki ; Nomura, R. ; Nemoto, H. ; Takeuchi, H. ; Yoshioka, H. ; Toda, K. ; Taniguchi, K. ; Amano, A. ; Ooshima, T. / Distribution of Porphyromonas gingivalis fimA genotypes in cardiovascular specimens from Japanese patients. In: Oral Microbiology and Immunology. 2008 ; Vol. 23, No. 2. pp. 170-172.
@article{ff22cc0831af43d7a824b26cf0dfaeb8,
title = "Distribution of Porphyromonas gingivalis fimA genotypes in cardiovascular specimens from Japanese patients",
abstract = "Introduction: Porphyromonas gingivalis, a major periodontal pathogen, is gaining increasing attention for its possible association with cardiovascular diseases. Its fimbriae are classified into six genotypes (types I-V and Ib) based on the diversity of the fimA genes encoding the fimbrial subunits. In this study, fimA genotypic distribution was analyzed in P. gingivalis-infected cardiovascular specimens. Methods: A total of 112 heart valves and 80 atheromatous plaque specimens were collected from patients undergoing cardiovascular surgery, as well as 56 dental plaque specimens. Bacterial DNA was extracted from each, and polymerase chain reaction analysis was carried out with a P. gingivalis-specific set of primers. P. gingivalis-positive specimens were further analyzed to discriminate the fimA genotype using polymerase chain reaction with fimA type-specific primer sets. Results: P. gingivalis was detected in 10.4{\%} of the cardiovascular specimens and 50.0{\%} of the dental plaque samples. In the latter, type II was most frequently detected (35.7{\%}), followed by types I (28.6{\%}) and IV (21.4{\%}), while types IV and II were detected with considerable frequencies of 45.0{\%} and 30.0{\%}, respectively, in the cardiovascular specimens. In contrast, the occurrence of type I was limited (5.0{\%}) in the cardiovascular specimens. Conclusion: These results suggest that specific fimA genotypic clones, which are reportedly associated with periodontitis, are also frequently harbored in cardiovascular specimens, indicating the possible involvement of type II and IV clones in the initiation and progression of cardiovascular diseases.",
keywords = "Cardiovascular specimens, FimA genotype, Polymerase chain reaction, Porphyromonas gingivalis",
author = "K. Nakano and Hiroaki Inaba and R. Nomura and H. Nemoto and H. Takeuchi and H. Yoshioka and K. Toda and K. Taniguchi and A. Amano and T. Ooshima",
year = "2008",
month = "4",
doi = "10.1111/j.1399-302X.2007.00406.x",
language = "English",
volume = "23",
pages = "170--172",
journal = "Molecular Oral Microbiology",
issn = "2041-1006",
publisher = "American Journal of Nursing Company",
number = "2",

}

TY - JOUR

T1 - Distribution of Porphyromonas gingivalis fimA genotypes in cardiovascular specimens from Japanese patients

AU - Nakano, K.

AU - Inaba, Hiroaki

AU - Nomura, R.

AU - Nemoto, H.

AU - Takeuchi, H.

AU - Yoshioka, H.

AU - Toda, K.

AU - Taniguchi, K.

AU - Amano, A.

AU - Ooshima, T.

PY - 2008/4

Y1 - 2008/4

N2 - Introduction: Porphyromonas gingivalis, a major periodontal pathogen, is gaining increasing attention for its possible association with cardiovascular diseases. Its fimbriae are classified into six genotypes (types I-V and Ib) based on the diversity of the fimA genes encoding the fimbrial subunits. In this study, fimA genotypic distribution was analyzed in P. gingivalis-infected cardiovascular specimens. Methods: A total of 112 heart valves and 80 atheromatous plaque specimens were collected from patients undergoing cardiovascular surgery, as well as 56 dental plaque specimens. Bacterial DNA was extracted from each, and polymerase chain reaction analysis was carried out with a P. gingivalis-specific set of primers. P. gingivalis-positive specimens were further analyzed to discriminate the fimA genotype using polymerase chain reaction with fimA type-specific primer sets. Results: P. gingivalis was detected in 10.4% of the cardiovascular specimens and 50.0% of the dental plaque samples. In the latter, type II was most frequently detected (35.7%), followed by types I (28.6%) and IV (21.4%), while types IV and II were detected with considerable frequencies of 45.0% and 30.0%, respectively, in the cardiovascular specimens. In contrast, the occurrence of type I was limited (5.0%) in the cardiovascular specimens. Conclusion: These results suggest that specific fimA genotypic clones, which are reportedly associated with periodontitis, are also frequently harbored in cardiovascular specimens, indicating the possible involvement of type II and IV clones in the initiation and progression of cardiovascular diseases.

AB - Introduction: Porphyromonas gingivalis, a major periodontal pathogen, is gaining increasing attention for its possible association with cardiovascular diseases. Its fimbriae are classified into six genotypes (types I-V and Ib) based on the diversity of the fimA genes encoding the fimbrial subunits. In this study, fimA genotypic distribution was analyzed in P. gingivalis-infected cardiovascular specimens. Methods: A total of 112 heart valves and 80 atheromatous plaque specimens were collected from patients undergoing cardiovascular surgery, as well as 56 dental plaque specimens. Bacterial DNA was extracted from each, and polymerase chain reaction analysis was carried out with a P. gingivalis-specific set of primers. P. gingivalis-positive specimens were further analyzed to discriminate the fimA genotype using polymerase chain reaction with fimA type-specific primer sets. Results: P. gingivalis was detected in 10.4% of the cardiovascular specimens and 50.0% of the dental plaque samples. In the latter, type II was most frequently detected (35.7%), followed by types I (28.6%) and IV (21.4%), while types IV and II were detected with considerable frequencies of 45.0% and 30.0%, respectively, in the cardiovascular specimens. In contrast, the occurrence of type I was limited (5.0%) in the cardiovascular specimens. Conclusion: These results suggest that specific fimA genotypic clones, which are reportedly associated with periodontitis, are also frequently harbored in cardiovascular specimens, indicating the possible involvement of type II and IV clones in the initiation and progression of cardiovascular diseases.

KW - Cardiovascular specimens

KW - FimA genotype

KW - Polymerase chain reaction

KW - Porphyromonas gingivalis

UR - http://www.scopus.com/inward/record.url?scp=39049127475&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=39049127475&partnerID=8YFLogxK

U2 - 10.1111/j.1399-302X.2007.00406.x

DO - 10.1111/j.1399-302X.2007.00406.x

M3 - Article

VL - 23

SP - 170

EP - 172

JO - Molecular Oral Microbiology

JF - Molecular Oral Microbiology

SN - 2041-1006

IS - 2

ER -