TY - JOUR
T1 - Distribution of plural HCV types in Japan
AU - Kato, Nobuyuki
AU - Ootsuyama, Yuko
AU - Ohkoshi, Showgo
AU - Nakazawa, Takahide
AU - Mori, Shigehisa
AU - Hijikata, Makoto
AU - Shimotohno, Kunitada
N1 - Funding Information:
ACKNOWLEDGMENTS: This study was supported by Grants-in-Aid for Cancer Research and for a Comprehensive 10-Year Strategy for Cancer Control from the Ministry of Health and Welfare of Japan. T.N. is the recipient of Research Resident Fellowships from the Foundation for Promotion of Cancer Research.
PY - 1991/11/27
Y1 - 1991/11/27
N2 - A detection system was developed to distinguish the four different HCV genomes[HCV-J, HCV-US, HCV-K2 and group II HCV(HCVGII)], involving reverse transcription followed by a nested polymerase chain reaction using specific primers for each HCV type. The putative non-structural(NS) 5 regions of HCV-J, HCV-US and HCVK2 and the putative NS3 region of HCV-GII were amplified. Of 95 specimens from patients with acute hepatitis, chronic hepatitis, liver cirrhosis or hepatocellular carcinoma, 67 specimens were positive for HCV-J, 2 for HCV-US, 23 for HCV-K2 and 11 for HCV-GII. About half the specimens that were positive for HCV-K2 or HCV-GII were coinfected with HCV-J and all those that were positive for HCV-GII were also positive for HCV-K2. Nucleotide sequence analysis of several amplified cDNA products revealed that HCV-K2 and HCV-GII could each be classified into two groups, and the pattern of classification of HCV-K2 was identical with that of HCV-GII. Therefore, our results strongly suggest that HCV-K2 is the same as HCV-GII.
AB - A detection system was developed to distinguish the four different HCV genomes[HCV-J, HCV-US, HCV-K2 and group II HCV(HCVGII)], involving reverse transcription followed by a nested polymerase chain reaction using specific primers for each HCV type. The putative non-structural(NS) 5 regions of HCV-J, HCV-US and HCVK2 and the putative NS3 region of HCV-GII were amplified. Of 95 specimens from patients with acute hepatitis, chronic hepatitis, liver cirrhosis or hepatocellular carcinoma, 67 specimens were positive for HCV-J, 2 for HCV-US, 23 for HCV-K2 and 11 for HCV-GII. About half the specimens that were positive for HCV-K2 or HCV-GII were coinfected with HCV-J and all those that were positive for HCV-GII were also positive for HCV-K2. Nucleotide sequence analysis of several amplified cDNA products revealed that HCV-K2 and HCV-GII could each be classified into two groups, and the pattern of classification of HCV-K2 was identical with that of HCV-GII. Therefore, our results strongly suggest that HCV-K2 is the same as HCV-GII.
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U2 - 10.1016/S0006-291X(05)81414-7
DO - 10.1016/S0006-291X(05)81414-7
M3 - Article
C2 - 1720309
AN - SCOPUS:0026355306
SN - 0006-291X
VL - 181
SP - 279
EP - 285
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 1
ER -
