Distribution of plural HCV types in Japan

Nobuyuki Kato, Yuko Ootsuyama, Showgo Ohkoshi, Takahide Nakazawa, Shigehisa Mori, Makoto Hijikata, Kunitada Shimotohno

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

A detection system was developed to distinguish the four different HCV genomes[HCV-J, HCV-US, HCV-K2 and group II HCV(HCVGII)], involving reverse transcription followed by a nested polymerase chain reaction using specific primers for each HCV type. The putative non-structural(NS) 5 regions of HCV-J, HCV-US and HCVK2 and the putative NS3 region of HCV-GII were amplified. Of 95 specimens from patients with acute hepatitis, chronic hepatitis, liver cirrhosis or hepatocellular carcinoma, 67 specimens were positive for HCV-J, 2 for HCV-US, 23 for HCV-K2 and 11 for HCV-GII. About half the specimens that were positive for HCV-K2 or HCV-GII were coinfected with HCV-J and all those that were positive for HCV-GII were also positive for HCV-K2. Nucleotide sequence analysis of several amplified cDNA products revealed that HCV-K2 and HCV-GII could each be classified into two groups, and the pattern of classification of HCV-K2 was identical with that of HCV-GII. Therefore, our results strongly suggest that HCV-K2 is the same as HCV-GII.

Original languageEnglish
Pages (from-to)279-285
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume181
Issue number1
DOIs
Publication statusPublished - Nov 27 1991
Externally publishedYes

Fingerprint

Polymerase chain reaction
Chronic Hepatitis
Transcription
Liver Cirrhosis
Liver
Hepatitis
Reverse Transcription
Sequence Analysis
Hepatocellular Carcinoma
Japan
Nucleotides
Complementary DNA
Genes
Genome
Polymerase Chain Reaction

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Kato, N., Ootsuyama, Y., Ohkoshi, S., Nakazawa, T., Mori, S., Hijikata, M., & Shimotohno, K. (1991). Distribution of plural HCV types in Japan. Biochemical and Biophysical Research Communications, 181(1), 279-285. https://doi.org/10.1016/S0006-291X(05)81414-7

Distribution of plural HCV types in Japan. / Kato, Nobuyuki; Ootsuyama, Yuko; Ohkoshi, Showgo; Nakazawa, Takahide; Mori, Shigehisa; Hijikata, Makoto; Shimotohno, Kunitada.

In: Biochemical and Biophysical Research Communications, Vol. 181, No. 1, 27.11.1991, p. 279-285.

Research output: Contribution to journalArticle

Kato, N, Ootsuyama, Y, Ohkoshi, S, Nakazawa, T, Mori, S, Hijikata, M & Shimotohno, K 1991, 'Distribution of plural HCV types in Japan', Biochemical and Biophysical Research Communications, vol. 181, no. 1, pp. 279-285. https://doi.org/10.1016/S0006-291X(05)81414-7
Kato N, Ootsuyama Y, Ohkoshi S, Nakazawa T, Mori S, Hijikata M et al. Distribution of plural HCV types in Japan. Biochemical and Biophysical Research Communications. 1991 Nov 27;181(1):279-285. https://doi.org/10.1016/S0006-291X(05)81414-7
Kato, Nobuyuki ; Ootsuyama, Yuko ; Ohkoshi, Showgo ; Nakazawa, Takahide ; Mori, Shigehisa ; Hijikata, Makoto ; Shimotohno, Kunitada. / Distribution of plural HCV types in Japan. In: Biochemical and Biophysical Research Communications. 1991 ; Vol. 181, No. 1. pp. 279-285.
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