Abstract
A detection system was developed to distinguish the four different HCV genomes[HCV-J, HCV-US, HCV-K2 and group II HCV(HCVGII)], involving reverse transcription followed by a nested polymerase chain reaction using specific primers for each HCV type. The putative non-structural(NS) 5 regions of HCV-J, HCV-US and HCVK2 and the putative NS3 region of HCV-GII were amplified. Of 95 specimens from patients with acute hepatitis, chronic hepatitis, liver cirrhosis or hepatocellular carcinoma, 67 specimens were positive for HCV-J, 2 for HCV-US, 23 for HCV-K2 and 11 for HCV-GII. About half the specimens that were positive for HCV-K2 or HCV-GII were coinfected with HCV-J and all those that were positive for HCV-GII were also positive for HCV-K2. Nucleotide sequence analysis of several amplified cDNA products revealed that HCV-K2 and HCV-GII could each be classified into two groups, and the pattern of classification of HCV-K2 was identical with that of HCV-GII. Therefore, our results strongly suggest that HCV-K2 is the same as HCV-GII.
| Original language | English |
|---|---|
| Pages (from-to) | 279-285 |
| Number of pages | 7 |
| Journal | Biochemical and Biophysical Research Communications |
| Volume | 181 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - Nov 27 1991 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Molecular Biology
Cite this
Distribution of plural HCV types in Japan. / Kato, Nobuyuki; Ootsuyama, Yuko; Ohkoshi, Showgo; Nakazawa, Takahide; Mori, Shigehisa; Hijikata, Makoto; Shimotohno, Kunitada.
In: Biochemical and Biophysical Research Communications, Vol. 181, No. 1, 27.11.1991, p. 279-285.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Distribution of plural HCV types in Japan
AU - Kato, Nobuyuki
AU - Ootsuyama, Yuko
AU - Ohkoshi, Showgo
AU - Nakazawa, Takahide
AU - Mori, Shigehisa
AU - Hijikata, Makoto
AU - Shimotohno, Kunitada
PY - 1991/11/27
Y1 - 1991/11/27
N2 - A detection system was developed to distinguish the four different HCV genomes[HCV-J, HCV-US, HCV-K2 and group II HCV(HCVGII)], involving reverse transcription followed by a nested polymerase chain reaction using specific primers for each HCV type. The putative non-structural(NS) 5 regions of HCV-J, HCV-US and HCVK2 and the putative NS3 region of HCV-GII were amplified. Of 95 specimens from patients with acute hepatitis, chronic hepatitis, liver cirrhosis or hepatocellular carcinoma, 67 specimens were positive for HCV-J, 2 for HCV-US, 23 for HCV-K2 and 11 for HCV-GII. About half the specimens that were positive for HCV-K2 or HCV-GII were coinfected with HCV-J and all those that were positive for HCV-GII were also positive for HCV-K2. Nucleotide sequence analysis of several amplified cDNA products revealed that HCV-K2 and HCV-GII could each be classified into two groups, and the pattern of classification of HCV-K2 was identical with that of HCV-GII. Therefore, our results strongly suggest that HCV-K2 is the same as HCV-GII.
AB - A detection system was developed to distinguish the four different HCV genomes[HCV-J, HCV-US, HCV-K2 and group II HCV(HCVGII)], involving reverse transcription followed by a nested polymerase chain reaction using specific primers for each HCV type. The putative non-structural(NS) 5 regions of HCV-J, HCV-US and HCVK2 and the putative NS3 region of HCV-GII were amplified. Of 95 specimens from patients with acute hepatitis, chronic hepatitis, liver cirrhosis or hepatocellular carcinoma, 67 specimens were positive for HCV-J, 2 for HCV-US, 23 for HCV-K2 and 11 for HCV-GII. About half the specimens that were positive for HCV-K2 or HCV-GII were coinfected with HCV-J and all those that were positive for HCV-GII were also positive for HCV-K2. Nucleotide sequence analysis of several amplified cDNA products revealed that HCV-K2 and HCV-GII could each be classified into two groups, and the pattern of classification of HCV-K2 was identical with that of HCV-GII. Therefore, our results strongly suggest that HCV-K2 is the same as HCV-GII.
UR - http://www.scopus.com/inward/record.url?scp=0026355306&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0026355306&partnerID=8YFLogxK
U2 - 10.1016/S0006-291X(05)81414-7
DO - 10.1016/S0006-291X(05)81414-7
M3 - Article
C2 - 1720309
AN - SCOPUS:0026355306
VL - 181
SP - 279
EP - 285
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 1
ER -