Distribution of Collagen Type IV α1-6 Chains and MMP in Oral Squamous Cell Carcinoma

Ryo Tamamura, Hitoshi Nagatsuka, You Jin Lee, Jing Xiao, Noriyuki Nagai, Ichiro Naito, Yoshikazu Sado, Toshiyuki Kawakami

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Destruction of basement membrane is an important element in invasion of cancer cells. Type IV collagen, the major component of basement membrane has six distinct α chains. Matrix metalloproteinases(MMPs) are enzymes that can resolve extracellular matrix. In this study, we investigated the localization of six α chains and MMPs in oral squamous cell carcinoma immunohistochemically. In well differentiated squamous cell carcinoma, the localization of α chains showed various patterns. α1 (IV),α2 (IV), α5 (IV) and α6 (IV) chains were stained in almost all basement membranes in the central of cancer. Unique staining with only α5 (IV) and α6 (IV) chains was also observed. In the invasive point of cancer, all a chains mainly became negative, but MMP-2 and MMP-9 were stained strongly. In poorly differentiated squamous cell carcinoma, both a chains and MMPs were not stained. Furthermore, we examined the correlation between distribution of α1 chain and invasive pattern of cancer. We found that the disappearence of α chains correlated closely with invasive activity of cancer.

Original languageEnglish
Pages (from-to)125-130
Number of pages6
JournalJournal of Hard Tissue Biology
Volume13
Issue number3
DOIs
Publication statusPublished - 2004
Externally publishedYes

Fingerprint

Collagen Type IV
Collagen Type I
Matrix Metalloproteinases
Collagen
Squamous Cell Carcinoma
Basement Membrane
Neoplasms
Matrix Metalloproteinase 2
Matrix Metalloproteinase 9
Cells
Extracellular Matrix
Enzymes
Epithelial Cells
Metalloproteases
Staining and Labeling

Keywords

  • basement membrane
  • immunohistochemistry
  • matrix metafloproteinase (MMP)
  • squamous cell carcinoma
  • type IV collagen

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Biochemistry
  • Biomaterials
  • Orthopedics and Sports Medicine
  • Dentistry(all)
  • Cell Biology

Cite this

Distribution of Collagen Type IV α1-6 Chains and MMP in Oral Squamous Cell Carcinoma. / Tamamura, Ryo; Nagatsuka, Hitoshi; Lee, You Jin; Xiao, Jing; Nagai, Noriyuki; Naito, Ichiro; Sado, Yoshikazu; Kawakami, Toshiyuki.

In: Journal of Hard Tissue Biology, Vol. 13, No. 3, 2004, p. 125-130.

Research output: Contribution to journalArticle

Tamamura, R, Nagatsuka, H, Lee, YJ, Xiao, J, Nagai, N, Naito, I, Sado, Y & Kawakami, T 2004, 'Distribution of Collagen Type IV α1-6 Chains and MMP in Oral Squamous Cell Carcinoma', Journal of Hard Tissue Biology, vol. 13, no. 3, pp. 125-130. https://doi.org/10.2485/jhtb.13.125
Tamamura, Ryo ; Nagatsuka, Hitoshi ; Lee, You Jin ; Xiao, Jing ; Nagai, Noriyuki ; Naito, Ichiro ; Sado, Yoshikazu ; Kawakami, Toshiyuki. / Distribution of Collagen Type IV α1-6 Chains and MMP in Oral Squamous Cell Carcinoma. In: Journal of Hard Tissue Biology. 2004 ; Vol. 13, No. 3. pp. 125-130.
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