TY - JOUR
T1 - Distribution of basement membrane type IV collagen α chains in ameloblastoma
T2 - An immunofluorescence study
AU - Nakano, Keisuke
AU - Siar, Chong Huat
AU - Nagai, Noriyuki
AU - Naito, Ichiro
AU - Sado, Yoshikazu
AU - Nagatsuka, Hitoshi
AU - Hoh, Choufuku
AU - Kurada, Katsuhiro
AU - Tsujigiwa, Hidetsugu
AU - Gunduz, Mehmet
PY - 2002/9
Y1 - 2002/9
N2 - Background: Type IV collagen, a heterotrimeric molecule that exists in six genetically distinct forms, α1(IV)-α6(IV) is a major structural component of basement membrane (BM) and acts as a scaffold for other BM constituents. Methods: Indirect immunofluorescence using α chain-specific monoclonal antibodies was employed to clarify basement membrane (BM) collagen IV distribution in two ameloblastoma, and for comparison, on oral mucosa and tooth germ. Results: Ameloblastoma BM expressed five of six genetically distinct forms of collagen IV: α1(IV), α2(IV), α5(IV) and α6(IV) chains occurred as intense linear stainings without disruption around neoplastic epithelium, and this expression pattern was fundamentally similar to oral mucosa BM; α4(IV) expression was rare and occurred around nests of primitive tumor cells or potentially invasive sites. The tooth germ demonstrated a stage- and position-specific collagen IV distribution: the inner enamel epithelium BM expressed α1(IV), α2(IV), and α4(IV) except in the cuspal predentine region; and the outer enamel epithelium BM expressed α1(IV), α2(IV), α5(IV), and α6(IV) chains. Conclusions: Results suggest that collagen IV α chain distribution in ameloblastoma BM plays an important role in tumor cytodifferentiation and progression.
AB - Background: Type IV collagen, a heterotrimeric molecule that exists in six genetically distinct forms, α1(IV)-α6(IV) is a major structural component of basement membrane (BM) and acts as a scaffold for other BM constituents. Methods: Indirect immunofluorescence using α chain-specific monoclonal antibodies was employed to clarify basement membrane (BM) collagen IV distribution in two ameloblastoma, and for comparison, on oral mucosa and tooth germ. Results: Ameloblastoma BM expressed five of six genetically distinct forms of collagen IV: α1(IV), α2(IV), α5(IV) and α6(IV) chains occurred as intense linear stainings without disruption around neoplastic epithelium, and this expression pattern was fundamentally similar to oral mucosa BM; α4(IV) expression was rare and occurred around nests of primitive tumor cells or potentially invasive sites. The tooth germ demonstrated a stage- and position-specific collagen IV distribution: the inner enamel epithelium BM expressed α1(IV), α2(IV), and α4(IV) except in the cuspal predentine region; and the outer enamel epithelium BM expressed α1(IV), α2(IV), α5(IV), and α6(IV) chains. Conclusions: Results suggest that collagen IV α chain distribution in ameloblastoma BM plays an important role in tumor cytodifferentiation and progression.
KW - Ameloblastoma
KW - Collagen IV profile
KW - Indirect immunofluorescence
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U2 - 10.1034/j.1600-0714.2002.00162.x
DO - 10.1034/j.1600-0714.2002.00162.x
M3 - Article
C2 - 12220358
AN - SCOPUS:18544366716
VL - 31
SP - 494
EP - 499
JO - Journal of Oral Pathology and Medicine
JF - Journal of Oral Pathology and Medicine
SN - 0904-2512
IS - 8
ER -