Distribution of Archaea in Japanese patients with periodontitis and humoral immune response to the components

Kokoro Yamabe, Hiroshi Maeda, Susumu Kokeguchi, Ichiro Tanimoto, Norihiro Sonoi, Susumu Asakawa, Shogo Takashiba

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

There is controversy regarding the existence of archaeal pathogens. Periodontitis is one of the human diseases in which Archaea have been suggested to have roles as pathogens. This study was performed to investigate the distribution of Archaea in Japanese patients with periodontitis and to examine the serum IgG responses to archaeal components. Subgingival plaque samples were collected from 111 periodontal pockets of 49 patients (17 with aggressive periodontitis and 32 with chronic periodontitis), and 30 subgingival plaque samples were collected from 17 healthy subjects. By PCR targeting the 16S rRNA gene, Archaea were detected in 15 plaque samples (13.5% of total samples) from 11 patients (29.4% of patients with aggressive periodontitis and 18.8% of patients with chronic periodontitis). Archaea were detected mostly (14/15) in severe diseased sites (pocket depth ≥6 mm), while no amplicons were observed in any samples from healthy controls. Sequence analysis of the PCR products revealed that the majority of Archaea in periodontal pockets were a Methanobrevibacter oralis-like phylotype. Western immunoblotting detected IgG antibodies against M. oralis in eight of the 11 sera from patients. These results suggest the potential of Archaea (M. oralis) as an antigenic pathogen of periodontitis.

Original languageEnglish
Pages (from-to)69-75
Number of pages7
JournalFEMS Microbiology Letters
Volume287
Issue number1
DOIs
Publication statusPublished - Oct 2008

Fingerprint

Periodontitis
Archaea
Humoral Immunity
Aggressive Periodontitis
Periodontal Pocket
Chronic Periodontitis
Methanobrevibacter
Immunoglobulin G
Polymerase Chain Reaction
Serum
rRNA Genes
Sequence Analysis
Healthy Volunteers
Western Blotting
Antibodies

Keywords

  • Archaea
  • Methanobrevibacter oralis
  • Periodontitis

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology
  • Applied Microbiology and Biotechnology
  • Microbiology

Cite this

Distribution of Archaea in Japanese patients with periodontitis and humoral immune response to the components. / Yamabe, Kokoro; Maeda, Hiroshi; Kokeguchi, Susumu; Tanimoto, Ichiro; Sonoi, Norihiro; Asakawa, Susumu; Takashiba, Shogo.

In: FEMS Microbiology Letters, Vol. 287, No. 1, 10.2008, p. 69-75.

Research output: Contribution to journalArticle

Yamabe, Kokoro ; Maeda, Hiroshi ; Kokeguchi, Susumu ; Tanimoto, Ichiro ; Sonoi, Norihiro ; Asakawa, Susumu ; Takashiba, Shogo. / Distribution of Archaea in Japanese patients with periodontitis and humoral immune response to the components. In: FEMS Microbiology Letters. 2008 ; Vol. 287, No. 1. pp. 69-75.
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