Abstract
Distribution of adrenomedullin (AM)-containing perivascular nerve fibers was studied in rat mesenteric arteries. Many fibers containing AM-like immunoreactivity (LI) were observed in the adventitia. AM-LI fibers were abolished by cold storage denervation or capsaicin but not 6-hydroxydopamine. Double immunostainings showed colocalization of AM-LI with calcitonin gene-related peptide (CGRP)-LI. The dorsal root ganglia had many AM-positive cells and AM mRNA detected by RT-PCR. Electron microscopy study revealed high proportions of immunogold labeling for AM and colocalization of both AM-LI and CGRP-LI in unmyelinated nerve axons. These results suggest that AM-containing perivascular nerves are distributed in the rat mesenteric artery.
| Original language | English |
|---|---|
| Pages (from-to) | 589-599 |
| Number of pages | 11 |
| Journal | Peptides |
| Volume | 25 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - Apr 2004 |
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Keywords
- Adrenomedullin
- Calcitonin gene-related peptide
- Capsaicin-sensitive nerve
- Neuropeptide Y
- Perivascular nerve
- Rat mesenteric artery
ASJC Scopus subject areas
- Biochemistry
- Endocrinology
- Physiology
- Cellular and Molecular Neuroscience
Cite this
Distribution of adrenomedullin-containing perivascular nerves in the rat mesenteric artery. / Hobara, N.; Nakamura, A.; Ohtsuka, Aiji; Narasaki, M.; Shibata, K.; Gomoita, Y.; Kawasaki, H.
In: Peptides, Vol. 25, No. 4, 04.2004, p. 589-599.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Distribution of adrenomedullin-containing perivascular nerves in the rat mesenteric artery
AU - Hobara, N.
AU - Nakamura, A.
AU - Ohtsuka, Aiji
AU - Narasaki, M.
AU - Shibata, K.
AU - Gomoita, Y.
AU - Kawasaki, H.
PY - 2004/4
Y1 - 2004/4
N2 - Distribution of adrenomedullin (AM)-containing perivascular nerve fibers was studied in rat mesenteric arteries. Many fibers containing AM-like immunoreactivity (LI) were observed in the adventitia. AM-LI fibers were abolished by cold storage denervation or capsaicin but not 6-hydroxydopamine. Double immunostainings showed colocalization of AM-LI with calcitonin gene-related peptide (CGRP)-LI. The dorsal root ganglia had many AM-positive cells and AM mRNA detected by RT-PCR. Electron microscopy study revealed high proportions of immunogold labeling for AM and colocalization of both AM-LI and CGRP-LI in unmyelinated nerve axons. These results suggest that AM-containing perivascular nerves are distributed in the rat mesenteric artery.
AB - Distribution of adrenomedullin (AM)-containing perivascular nerve fibers was studied in rat mesenteric arteries. Many fibers containing AM-like immunoreactivity (LI) were observed in the adventitia. AM-LI fibers were abolished by cold storage denervation or capsaicin but not 6-hydroxydopamine. Double immunostainings showed colocalization of AM-LI with calcitonin gene-related peptide (CGRP)-LI. The dorsal root ganglia had many AM-positive cells and AM mRNA detected by RT-PCR. Electron microscopy study revealed high proportions of immunogold labeling for AM and colocalization of both AM-LI and CGRP-LI in unmyelinated nerve axons. These results suggest that AM-containing perivascular nerves are distributed in the rat mesenteric artery.
KW - Adrenomedullin
KW - Calcitonin gene-related peptide
KW - Capsaicin-sensitive nerve
KW - Neuropeptide Y
KW - Perivascular nerve
KW - Rat mesenteric artery
UR - http://www.scopus.com/inward/record.url?scp=2542422735&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=2542422735&partnerID=8YFLogxK
U2 - 10.1016/j.peptides.2004.02.005
DO - 10.1016/j.peptides.2004.02.005
M3 - Article
VL - 25
SP - 589
EP - 599
JO - Peptides
JF - Peptides
SN - 0196-9781
IS - 4
ER -