Distribution of adrenomedullin-containing perivascular nerves in the rat mesenteric artery

N. Hobara; A. Nakamura; A. Ohtsuka; M. Narasaki; K. Shibata; Y. Gomoita; H. Kawasaki

doi:10.1016/j.peptides.2004.02.005

Distribution of adrenomedullin-containing perivascular nerves in the rat mesenteric artery

N. Hobara, A. Nakamura, A. Ohtsuka, M. Narasaki, K. Shibata, Y. Gomoita, H. Kawasaki

Research output: Contribution to journal › Article

27 Citations (Scopus)

Abstract

Distribution of adrenomedullin (AM)-containing perivascular nerve fibers was studied in rat mesenteric arteries. Many fibers containing AM-like immunoreactivity (LI) were observed in the adventitia. AM-LI fibers were abolished by cold storage denervation or capsaicin but not 6-hydroxydopamine. Double immunostainings showed colocalization of AM-LI with calcitonin gene-related peptide (CGRP)-LI. The dorsal root ganglia had many AM-positive cells and AM mRNA detected by RT-PCR. Electron microscopy study revealed high proportions of immunogold labeling for AM and colocalization of both AM-LI and CGRP-LI in unmyelinated nerve axons. These results suggest that AM-containing perivascular nerves are distributed in the rat mesenteric artery.

Original language	English
Pages (from-to)	589-599
Number of pages	11
Journal	Peptides
Volume	25
Issue number	4
DOIs	https://doi.org/10.1016/j.peptides.2004.02.005
Publication status	Published - Apr 1 2004

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Keywords

Adrenomedullin
Calcitonin gene-related peptide
Capsaicin-sensitive nerve
Neuropeptide Y
Perivascular nerve
Rat mesenteric artery

ASJC Scopus subject areas

Biochemistry
Physiology
Endocrinology
Cellular and Molecular Neuroscience

Cite this

Hobara, N., Nakamura, A., Ohtsuka, A., Narasaki, M., Shibata, K., Gomoita, Y., & Kawasaki, H. (2004). Distribution of adrenomedullin-containing perivascular nerves in the rat mesenteric artery. Peptides, 25(4), 589-599. https://doi.org/10.1016/j.peptides.2004.02.005

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title = "Distribution of adrenomedullin-containing perivascular nerves in the rat mesenteric artery",

abstract = "Distribution of adrenomedullin (AM)-containing perivascular nerve fibers was studied in rat mesenteric arteries. Many fibers containing AM-like immunoreactivity (LI) were observed in the adventitia. AM-LI fibers were abolished by cold storage denervation or capsaicin but not 6-hydroxydopamine. Double immunostainings showed colocalization of AM-LI with calcitonin gene-related peptide (CGRP)-LI. The dorsal root ganglia had many AM-positive cells and AM mRNA detected by RT-PCR. Electron microscopy study revealed high proportions of immunogold labeling for AM and colocalization of both AM-LI and CGRP-LI in unmyelinated nerve axons. These results suggest that AM-containing perivascular nerves are distributed in the rat mesenteric artery.",

keywords = "Adrenomedullin, Calcitonin gene-related peptide, Capsaicin-sensitive nerve, Neuropeptide Y, Perivascular nerve, Rat mesenteric artery",

author = "N. Hobara and A. Nakamura and A. Ohtsuka and M. Narasaki and K. Shibata and Y. Gomoita and H. Kawasaki",

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AU - Hobara, N.

AU - Nakamura, A.

AU - Ohtsuka, A.

AU - Narasaki, M.

AU - Shibata, K.

AU - Gomoita, Y.

AU - Kawasaki, H.

PY - 2004/4/1

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N2 - Distribution of adrenomedullin (AM)-containing perivascular nerve fibers was studied in rat mesenteric arteries. Many fibers containing AM-like immunoreactivity (LI) were observed in the adventitia. AM-LI fibers were abolished by cold storage denervation or capsaicin but not 6-hydroxydopamine. Double immunostainings showed colocalization of AM-LI with calcitonin gene-related peptide (CGRP)-LI. The dorsal root ganglia had many AM-positive cells and AM mRNA detected by RT-PCR. Electron microscopy study revealed high proportions of immunogold labeling for AM and colocalization of both AM-LI and CGRP-LI in unmyelinated nerve axons. These results suggest that AM-containing perivascular nerves are distributed in the rat mesenteric artery.

AB - Distribution of adrenomedullin (AM)-containing perivascular nerve fibers was studied in rat mesenteric arteries. Many fibers containing AM-like immunoreactivity (LI) were observed in the adventitia. AM-LI fibers were abolished by cold storage denervation or capsaicin but not 6-hydroxydopamine. Double immunostainings showed colocalization of AM-LI with calcitonin gene-related peptide (CGRP)-LI. The dorsal root ganglia had many AM-positive cells and AM mRNA detected by RT-PCR. Electron microscopy study revealed high proportions of immunogold labeling for AM and colocalization of both AM-LI and CGRP-LI in unmyelinated nerve axons. These results suggest that AM-containing perivascular nerves are distributed in the rat mesenteric artery.

KW - Adrenomedullin

KW - Calcitonin gene-related peptide

KW - Capsaicin-sensitive nerve

KW - Neuropeptide Y

KW - Perivascular nerve

KW - Rat mesenteric artery

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Access to Document

10.1016/j.peptides.2004.02.005