Distribution of 10 periodontal bacterial species in children and adolescents over a 7-year period

K. Nakano, E. Miyamoto, K. Tamura, H. Nemoto, K. Fujita, R. Nomura, T. Ooshima

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Objective: There is scant information available regarding the distribution of periodontal bacterial species in children and adolescents over an extended period. The purpose of this study was to compare bacterial profiles in the same individuals over a period of 7 years. Subject and methods: Twenty-six children and adolescents from whom dental plaque and saliva specimens were obtained during both the first (1999-2000) and second (2006-2007) periods, were analyzed. Bacterial DNA was extracted from each specimen and the presence of 10 periodontal bacterial species was determined using a PCR method, with a focus on the red complex species of Porphyromonas gingivalis, Treponema denticola, and Tannerella forsythia. Results: Subjects with red complex species in saliva specimens obtained during the second collection possessed a significantly higher number of total bacterial species than those without. The detection rate of the red complex species in the second collection period samples was significantly greater in subjects who had two or more species detected in samples taken during the first collection compared with the other subjects. Conclusion: Subjects possessing red complex species may be at possible risk for infection with a high number of periodontal bacterial species during adolescent and younger adult years.

Original languageEnglish
Pages (from-to)658-664
Number of pages7
JournalOral Diseases
Volume14
Issue number7
DOIs
Publication statusPublished - Oct 2008
Externally publishedYes

Fingerprint

Saliva
Treponema denticola
Bacterial DNA
Dental Plaque
Porphyromonas gingivalis
Young Adult
Polymerase Chain Reaction
Infection
Tannerella forsythia

Keywords

  • Epidemiology
  • Inflammatory diseases
  • Molecular biology

ASJC Scopus subject areas

  • Dentistry(all)
  • Otorhinolaryngology

Cite this

Nakano, K., Miyamoto, E., Tamura, K., Nemoto, H., Fujita, K., Nomura, R., & Ooshima, T. (2008). Distribution of 10 periodontal bacterial species in children and adolescents over a 7-year period. Oral Diseases, 14(7), 658-664. https://doi.org/10.1111/j.1601-0825.2008.01452.x

Distribution of 10 periodontal bacterial species in children and adolescents over a 7-year period. / Nakano, K.; Miyamoto, E.; Tamura, K.; Nemoto, H.; Fujita, K.; Nomura, R.; Ooshima, T.

In: Oral Diseases, Vol. 14, No. 7, 10.2008, p. 658-664.

Research output: Contribution to journalArticle

Nakano, K, Miyamoto, E, Tamura, K, Nemoto, H, Fujita, K, Nomura, R & Ooshima, T 2008, 'Distribution of 10 periodontal bacterial species in children and adolescents over a 7-year period', Oral Diseases, vol. 14, no. 7, pp. 658-664. https://doi.org/10.1111/j.1601-0825.2008.01452.x
Nakano, K. ; Miyamoto, E. ; Tamura, K. ; Nemoto, H. ; Fujita, K. ; Nomura, R. ; Ooshima, T. / Distribution of 10 periodontal bacterial species in children and adolescents over a 7-year period. In: Oral Diseases. 2008 ; Vol. 14, No. 7. pp. 658-664.
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