Distribution of α(IV) collagen chains in the ocular anterior segments of adult mice

Kenji Saito, Tomoko Yonezawa, Jun Minaguchi, Masae Kurosaki, Shiho Suetsugu, Ai Nakajima, Hiroyuki Nomoto, Yuki Morizane, Yoshikazu Sado, Manabu Sugimoto, Shozo Kusachi, Yoshifumi Ninomiya

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9 Citations (Scopus)

Abstract

The distribution of the collagen chains from α1(IV) to α6(IV) could serve as a basis for the characterization of type IV collagen. In this study, immunohistochemistry of the ocular anterior segment of adult mice was performed using specific monoclonal antibodies against each chain in the series from α1(IV) to α6(IV). The results show that the components of type IV collagen in vascular basement membranes are α1(IV) and α2(IV) with or without α5(IV) and α6(IV) chains and those in epithelium and muscle basement membranes are α1(IV), α2(IV), α5(IV), and α6(IV) chains. In corneal endothelium, pigmented epithelium of iris and ciliary body, and trabecular meshwork, α3(IV) and α4(IV) chains are also expressed in addition to α1(IV), α2(IV), α5(IV), and α6(IV) chains. Moreover, we investigated the change in molecular composition in ciliary body during postnatal development. α3(IV) and α4(IV) chains were also expressed in addition to α1(IV), α2(IV), α5(IV), and α6(IV) chains in ciliary pigmented epithelium basement membrane from 7 days after birth. This result suggests that the basement membranes gradually change their biochemical features owing to temporal regulation. Taken together, these findings suggest that the different distribution and the developmental expression of α1(IV) to α6(IV) chains are associated with the tissue-specific function of type IV collagen in basement membranes.

Original languageEnglish
Pages (from-to)147-156
Number of pages10
JournalConnective Tissue Research
Volume52
Issue number2
DOIs
Publication statusPublished - Apr 2011

Keywords

  • basement membrane
  • ciliary body
  • collagen
  • extracellular matrix
  • eye

ASJC Scopus subject areas

  • Rheumatology
  • Biochemistry
  • Orthopedics and Sports Medicine
  • Molecular Biology
  • Cell Biology

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    Saito, K., Yonezawa, T., Minaguchi, J., Kurosaki, M., Suetsugu, S., Nakajima, A., Nomoto, H., Morizane, Y., Sado, Y., Sugimoto, M., Kusachi, S., & Ninomiya, Y. (2011). Distribution of α(IV) collagen chains in the ocular anterior segments of adult mice. Connective Tissue Research, 52(2), 147-156. https://doi.org/10.3109/03008207.2010.492062