Distinct human and mouse membrane trafficking systems for sweet taste receptors T1r2 and T1r3

Madoka Shimizu, Masao Goto, Takayuki Kawai, Atsuko Yamashita, Yuko Kusakabe

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

The sweet taste receptors T1r2 and T1r3 are included in the T1r taste receptor family that belongs to class C of the G protein-coupled receptors. Heterodimerization of T1r2 and T1r3 is required for the perception of sweet substances, but little is known about the mechanisms underlying this heterodimerization, including membrane trafficking. We developed tagged mouse T1r2 and T1r3, and human T1R2 and T1R3 and evaluated membrane trafficking in human embryonic kidney 293 (HEK293) cells. We found that human T1R3 surface expression was only observed when human T1R3 was coexpressed with human T1R2, whereas mouse T1r3 was expressed without mouse T1r2 expression. A domain-swapped chimera and truncated human T1R3 mutant showed that the Venus flytrap module and cysteine-rich domain (CRD) of human T1R3 contain a region related to the inhibition of human T1R3 membrane trafficking and coordinated regulation of human T1R3 membrane trafficking. We also found that the Venus flytrap module of both human T1R2 and T1R3 are needed for membrane trafficking, suggesting that the coexpression of human T1R2 and T1R3 is required for this event. These results suggest that the Venus flytrap module and CRD receive taste substances and play roles in membrane trafficking of human T1R2 and T1R3. These features are different from those of mouse receptors, indicating that human T1R2 and T1R3 are likely to have a novel membrane trafficking system.

Original languageEnglish
Article numbere100425
JournalPLoS One
Volume9
Issue number7
DOIs
Publication statusPublished - Jul 16 2014

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Membranes
mice
Droseraceae
Dionaea muscipula
Human Trafficking
Cysteine
G-Protein-Coupled Receptors
taste receptors
cysteine
chimerism
kidney cells
Kidney
mutants
receptors

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Distinct human and mouse membrane trafficking systems for sweet taste receptors T1r2 and T1r3. / Shimizu, Madoka; Goto, Masao; Kawai, Takayuki; Yamashita, Atsuko; Kusakabe, Yuko.

In: PLoS One, Vol. 9, No. 7, e100425, 16.07.2014.

Research output: Contribution to journalArticle

Shimizu, Madoka ; Goto, Masao ; Kawai, Takayuki ; Yamashita, Atsuko ; Kusakabe, Yuko. / Distinct human and mouse membrane trafficking systems for sweet taste receptors T1r2 and T1r3. In: PLoS One. 2014 ; Vol. 9, No. 7.
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