Distinct distribution of protein disulfide isomerase family proteins in rat tissues

Ken Ichi Iida, Osamu Miyaishi, Yosuke Iwata, Ken Ichi Kozaki, Mutsushi Matsuyama, Shinsuke Saga

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

We investigated the distribution of protein disulfide isomerase (PDI) family proteins PDI, ERp72, and ERp61 in rat tissues and compared their localization by immunohistochemical double staining. The extent of their expression was diverse in different cell types, although they were ubiquitously distributed in a wide variety of cell types. Prominent staining for all three proteins was detected in thyroid follicular epithelia, tracheal mucous gland cells and chondrocytes, and chief cells and mucous neck cells of the stomach. Hepatocytes, neuronal cells in brain and spinal cord, and pancreatic acinar cells were also consistently and uniformly stained, but with low intensity. On the other hand, distinct differences were observed for the expression of the three proteins in plasma cells, pancreatic islet cells, goblet cells, and Paneth's cells of intestines, seminiferous epithelia, and salivary gland ductal epithelia. The similarities and differences in the distribution of the three proteins provide helpful clues to their biological functions.

Original languageEnglish
Pages (from-to)751-759
Number of pages9
JournalJournal of Histochemistry and Cytochemistry
Volume44
Issue number7
Publication statusPublished - Jul 1996
Externally publishedYes

Fingerprint

Protein Disulfide-Isomerases
Proteins
Islets of Langerhans
Epithelium
Paneth Cells
Staining and Labeling
Seminiferous Epithelium
Goblet Cells
Acinar Cells
Chondrocytes
Plasma Cells
Salivary Glands
Intestines
Hepatocytes
Spinal Cord
Stomach
Thyroid Gland
Neck
Brain

Keywords

  • ERp61
  • ERp72
  • Immunofluorescence
  • Protein disulfide isomerase (PDI)
  • Rat tissues
  • Tissue distribution

ASJC Scopus subject areas

  • Anatomy
  • Cell Biology

Cite this

Iida, K. I., Miyaishi, O., Iwata, Y., Kozaki, K. I., Matsuyama, M., & Saga, S. (1996). Distinct distribution of protein disulfide isomerase family proteins in rat tissues. Journal of Histochemistry and Cytochemistry, 44(7), 751-759.

Distinct distribution of protein disulfide isomerase family proteins in rat tissues. / Iida, Ken Ichi; Miyaishi, Osamu; Iwata, Yosuke; Kozaki, Ken Ichi; Matsuyama, Mutsushi; Saga, Shinsuke.

In: Journal of Histochemistry and Cytochemistry, Vol. 44, No. 7, 07.1996, p. 751-759.

Research output: Contribution to journalArticle

Iida, KI, Miyaishi, O, Iwata, Y, Kozaki, KI, Matsuyama, M & Saga, S 1996, 'Distinct distribution of protein disulfide isomerase family proteins in rat tissues', Journal of Histochemistry and Cytochemistry, vol. 44, no. 7, pp. 751-759.
Iida KI, Miyaishi O, Iwata Y, Kozaki KI, Matsuyama M, Saga S. Distinct distribution of protein disulfide isomerase family proteins in rat tissues. Journal of Histochemistry and Cytochemistry. 1996 Jul;44(7):751-759.
Iida, Ken Ichi ; Miyaishi, Osamu ; Iwata, Yosuke ; Kozaki, Ken Ichi ; Matsuyama, Mutsushi ; Saga, Shinsuke. / Distinct distribution of protein disulfide isomerase family proteins in rat tissues. In: Journal of Histochemistry and Cytochemistry. 1996 ; Vol. 44, No. 7. pp. 751-759.
@article{a7cf1214c5e44c23becb91f95b29ce5f,
title = "Distinct distribution of protein disulfide isomerase family proteins in rat tissues",
abstract = "We investigated the distribution of protein disulfide isomerase (PDI) family proteins PDI, ERp72, and ERp61 in rat tissues and compared their localization by immunohistochemical double staining. The extent of their expression was diverse in different cell types, although they were ubiquitously distributed in a wide variety of cell types. Prominent staining for all three proteins was detected in thyroid follicular epithelia, tracheal mucous gland cells and chondrocytes, and chief cells and mucous neck cells of the stomach. Hepatocytes, neuronal cells in brain and spinal cord, and pancreatic acinar cells were also consistently and uniformly stained, but with low intensity. On the other hand, distinct differences were observed for the expression of the three proteins in plasma cells, pancreatic islet cells, goblet cells, and Paneth's cells of intestines, seminiferous epithelia, and salivary gland ductal epithelia. The similarities and differences in the distribution of the three proteins provide helpful clues to their biological functions.",
keywords = "ERp61, ERp72, Immunofluorescence, Protein disulfide isomerase (PDI), Rat tissues, Tissue distribution",
author = "Iida, {Ken Ichi} and Osamu Miyaishi and Yosuke Iwata and Kozaki, {Ken Ichi} and Mutsushi Matsuyama and Shinsuke Saga",
year = "1996",
month = "7",
language = "English",
volume = "44",
pages = "751--759",
journal = "Journal of Histochemistry and Cytochemistry",
issn = "0022-1554",
publisher = "Histochemical Society Inc.",
number = "7",

}

TY - JOUR

T1 - Distinct distribution of protein disulfide isomerase family proteins in rat tissues

AU - Iida, Ken Ichi

AU - Miyaishi, Osamu

AU - Iwata, Yosuke

AU - Kozaki, Ken Ichi

AU - Matsuyama, Mutsushi

AU - Saga, Shinsuke

PY - 1996/7

Y1 - 1996/7

N2 - We investigated the distribution of protein disulfide isomerase (PDI) family proteins PDI, ERp72, and ERp61 in rat tissues and compared their localization by immunohistochemical double staining. The extent of their expression was diverse in different cell types, although they were ubiquitously distributed in a wide variety of cell types. Prominent staining for all three proteins was detected in thyroid follicular epithelia, tracheal mucous gland cells and chondrocytes, and chief cells and mucous neck cells of the stomach. Hepatocytes, neuronal cells in brain and spinal cord, and pancreatic acinar cells were also consistently and uniformly stained, but with low intensity. On the other hand, distinct differences were observed for the expression of the three proteins in plasma cells, pancreatic islet cells, goblet cells, and Paneth's cells of intestines, seminiferous epithelia, and salivary gland ductal epithelia. The similarities and differences in the distribution of the three proteins provide helpful clues to their biological functions.

AB - We investigated the distribution of protein disulfide isomerase (PDI) family proteins PDI, ERp72, and ERp61 in rat tissues and compared their localization by immunohistochemical double staining. The extent of their expression was diverse in different cell types, although they were ubiquitously distributed in a wide variety of cell types. Prominent staining for all three proteins was detected in thyroid follicular epithelia, tracheal mucous gland cells and chondrocytes, and chief cells and mucous neck cells of the stomach. Hepatocytes, neuronal cells in brain and spinal cord, and pancreatic acinar cells were also consistently and uniformly stained, but with low intensity. On the other hand, distinct differences were observed for the expression of the three proteins in plasma cells, pancreatic islet cells, goblet cells, and Paneth's cells of intestines, seminiferous epithelia, and salivary gland ductal epithelia. The similarities and differences in the distribution of the three proteins provide helpful clues to their biological functions.

KW - ERp61

KW - ERp72

KW - Immunofluorescence

KW - Protein disulfide isomerase (PDI)

KW - Rat tissues

KW - Tissue distribution

UR - http://www.scopus.com/inward/record.url?scp=0029943402&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029943402&partnerID=8YFLogxK

M3 - Article

VL - 44

SP - 751

EP - 759

JO - Journal of Histochemistry and Cytochemistry

JF - Journal of Histochemistry and Cytochemistry

SN - 0022-1554

IS - 7

ER -