Abstract
We investigated the distribution of protein disulfide isomerase (PDI) family proteins PDI, ERp72, and ERp61 in rat tissues and compared their localization by immunohistochemical double staining. The extent of their expression was diverse in different cell types, although they were ubiquitously distributed in a wide variety of cell types. Prominent staining for all three proteins was detected in thyroid follicular epithelia, tracheal mucous gland cells and chondrocytes, and chief cells and mucous neck cells of the stomach. Hepatocytes, neuronal cells in brain and spinal cord, and pancreatic acinar cells were also consistently and uniformly stained, but with low intensity. On the other hand, distinct differences were observed for the expression of the three proteins in plasma cells, pancreatic islet cells, goblet cells, and Paneth's cells of intestines, seminiferous epithelia, and salivary gland ductal epithelia. The similarities and differences in the distribution of the three proteins provide helpful clues to their biological functions.
| Original language | English |
|---|---|
| Pages (from-to) | 751-759 |
| Number of pages | 9 |
| Journal | Journal of Histochemistry and Cytochemistry |
| Volume | 44 |
| Issue number | 7 |
| Publication status | Published - Jul 1996 |
| Externally published | Yes |
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Keywords
- ERp61
- ERp72
- Immunofluorescence
- Protein disulfide isomerase (PDI)
- Rat tissues
- Tissue distribution
ASJC Scopus subject areas
- Anatomy
- Cell Biology
Cite this
Distinct distribution of protein disulfide isomerase family proteins in rat tissues. / Iida, Ken Ichi; Miyaishi, Osamu; Iwata, Yosuke; Kozaki, Ken Ichi; Matsuyama, Mutsushi; Saga, Shinsuke.
In: Journal of Histochemistry and Cytochemistry, Vol. 44, No. 7, 07.1996, p. 751-759.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Distinct distribution of protein disulfide isomerase family proteins in rat tissues
AU - Iida, Ken Ichi
AU - Miyaishi, Osamu
AU - Iwata, Yosuke
AU - Kozaki, Ken Ichi
AU - Matsuyama, Mutsushi
AU - Saga, Shinsuke
PY - 1996/7
Y1 - 1996/7
N2 - We investigated the distribution of protein disulfide isomerase (PDI) family proteins PDI, ERp72, and ERp61 in rat tissues and compared their localization by immunohistochemical double staining. The extent of their expression was diverse in different cell types, although they were ubiquitously distributed in a wide variety of cell types. Prominent staining for all three proteins was detected in thyroid follicular epithelia, tracheal mucous gland cells and chondrocytes, and chief cells and mucous neck cells of the stomach. Hepatocytes, neuronal cells in brain and spinal cord, and pancreatic acinar cells were also consistently and uniformly stained, but with low intensity. On the other hand, distinct differences were observed for the expression of the three proteins in plasma cells, pancreatic islet cells, goblet cells, and Paneth's cells of intestines, seminiferous epithelia, and salivary gland ductal epithelia. The similarities and differences in the distribution of the three proteins provide helpful clues to their biological functions.
AB - We investigated the distribution of protein disulfide isomerase (PDI) family proteins PDI, ERp72, and ERp61 in rat tissues and compared their localization by immunohistochemical double staining. The extent of their expression was diverse in different cell types, although they were ubiquitously distributed in a wide variety of cell types. Prominent staining for all three proteins was detected in thyroid follicular epithelia, tracheal mucous gland cells and chondrocytes, and chief cells and mucous neck cells of the stomach. Hepatocytes, neuronal cells in brain and spinal cord, and pancreatic acinar cells were also consistently and uniformly stained, but with low intensity. On the other hand, distinct differences were observed for the expression of the three proteins in plasma cells, pancreatic islet cells, goblet cells, and Paneth's cells of intestines, seminiferous epithelia, and salivary gland ductal epithelia. The similarities and differences in the distribution of the three proteins provide helpful clues to their biological functions.
KW - ERp61
KW - ERp72
KW - Immunofluorescence
KW - Protein disulfide isomerase (PDI)
KW - Rat tissues
KW - Tissue distribution
UR - http://www.scopus.com/inward/record.url?scp=0029943402&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0029943402&partnerID=8YFLogxK
M3 - Article
VL - 44
SP - 751
EP - 759
JO - Journal of Histochemistry and Cytochemistry
JF - Journal of Histochemistry and Cytochemistry
SN - 0022-1554
IS - 7
ER -