Dissociation of SNAP-25 and VAMP-2 by MgATP in permeabilized adrenal chromaffin cells

Hiroaki Misonou; Tei ichi Nishiki; Mariko Sekiguchi; Masami Takahashi; Yoichi Kamata; Shunji Kozaki; Mica Ohara-Imaizumi; Konosuke Kumakura

doi:10.1016/0006-8993(96)00954-7

Dissociation of SNAP-25 and VAMP-2 by MgATP in permeabilized adrenal chromaffin cells

Hiroaki Misonou, Tei ichi Nishiki, Mariko Sekiguchi, Masami Takahashi, Yoichi Kamata, Shunji Kozaki, Mica Ohara-Imaizumi, Konosuke Kumakura

Graduate School of Medicine Dentistry and Pharmaceutical Sciences

Research output: Contribution to journal › Article › peer-review

12 Citations (Scopus)

Abstract

In digitonin-permeabilized adrenal chromaffin cells, Ca²⁺-induced catecholamine release can be resolved into at least two sequential steps: a MgATP-dependent priming step and a MgATP-independent Ca²⁺-triggered step. Botulinum neurotoxins types A and E cleaved SNAP-25, and blocked MgATP-independent Ca²⁺-induced catecholamine release from the permeabilized chromaffin cells. When the permeabilized cells were primed by pretreatment with MgATP, the amount of SNAP-25 associated with VAMP-2 decreased, and the fraction of SNAP-25 proteolyzed by the neurotoxins increased. These results suggest that dissociation of SNAP-25 and VAMP-2 occurs during the MgATP-dependent priming step, and SNAP-25 plays some important roles in the subsequent MgATP-independent step.

Original language	English
Pages (from-to)	351-355
Number of pages	5
Journal	Brain Research
Volume	737
Issue number	1-2
DOIs	https://doi.org/10.1016/0006-8993(96)00954-7
Publication status	Published - Oct 21 1996

Keywords

Botulinum neurotoxin
Exocytosis
MgATP
SNAP-24
VAMP-2

ASJC Scopus subject areas

Neuroscience(all)
Molecular Biology
Clinical Neurology
Developmental Biology

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title = "Dissociation of SNAP-25 and VAMP-2 by MgATP in permeabilized adrenal chromaffin cells",

abstract = "In digitonin-permeabilized adrenal chromaffin cells, Ca2+-induced catecholamine release can be resolved into at least two sequential steps: a MgATP-dependent priming step and a MgATP-independent Ca2+-triggered step. Botulinum neurotoxins types A and E cleaved SNAP-25, and blocked MgATP-independent Ca2+-induced catecholamine release from the permeabilized chromaffin cells. When the permeabilized cells were primed by pretreatment with MgATP, the amount of SNAP-25 associated with VAMP-2 decreased, and the fraction of SNAP-25 proteolyzed by the neurotoxins increased. These results suggest that dissociation of SNAP-25 and VAMP-2 occurs during the MgATP-dependent priming step, and SNAP-25 plays some important roles in the subsequent MgATP-independent step.",

keywords = "Botulinum neurotoxin, Exocytosis, MgATP, SNAP-24, VAMP-2",

author = "Hiroaki Misonou and Nishiki, {Tei ichi} and Mariko Sekiguchi and Masami Takahashi and Yoichi Kamata and Shunji Kozaki and Mica Ohara-Imaizumi and Konosuke Kumakura",

year = "1996",

month = oct,

day = "21",

doi = "10.1016/0006-8993(96)00954-7",

language = "English",

volume = "737",

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T1 - Dissociation of SNAP-25 and VAMP-2 by MgATP in permeabilized adrenal chromaffin cells

AU - Misonou, Hiroaki

AU - Nishiki, Tei ichi

AU - Sekiguchi, Mariko

AU - Takahashi, Masami

AU - Kamata, Yoichi

AU - Kozaki, Shunji

AU - Ohara-Imaizumi, Mica

AU - Kumakura, Konosuke

PY - 1996/10/21

Y1 - 1996/10/21

N2 - In digitonin-permeabilized adrenal chromaffin cells, Ca2+-induced catecholamine release can be resolved into at least two sequential steps: a MgATP-dependent priming step and a MgATP-independent Ca2+-triggered step. Botulinum neurotoxins types A and E cleaved SNAP-25, and blocked MgATP-independent Ca2+-induced catecholamine release from the permeabilized chromaffin cells. When the permeabilized cells were primed by pretreatment with MgATP, the amount of SNAP-25 associated with VAMP-2 decreased, and the fraction of SNAP-25 proteolyzed by the neurotoxins increased. These results suggest that dissociation of SNAP-25 and VAMP-2 occurs during the MgATP-dependent priming step, and SNAP-25 plays some important roles in the subsequent MgATP-independent step.

AB - In digitonin-permeabilized adrenal chromaffin cells, Ca2+-induced catecholamine release can be resolved into at least two sequential steps: a MgATP-dependent priming step and a MgATP-independent Ca2+-triggered step. Botulinum neurotoxins types A and E cleaved SNAP-25, and blocked MgATP-independent Ca2+-induced catecholamine release from the permeabilized chromaffin cells. When the permeabilized cells were primed by pretreatment with MgATP, the amount of SNAP-25 associated with VAMP-2 decreased, and the fraction of SNAP-25 proteolyzed by the neurotoxins increased. These results suggest that dissociation of SNAP-25 and VAMP-2 occurs during the MgATP-dependent priming step, and SNAP-25 plays some important roles in the subsequent MgATP-independent step.

KW - Botulinum neurotoxin

KW - Exocytosis

KW - MgATP

KW - SNAP-24

KW - VAMP-2

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