Dissociation of SNAP-25 and VAMP-2 by MgATP in permeabilized adrenal chromaffin cells

Hiroaki Misonou, Tei ichi Nishiki, Mariko Sekiguchi, Masami Takahashi, Yoichi Kamata, Shunji Kozaki, Mica Ohara-Imaizumi, Konosuke Kumakura

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12 Citations (Scopus)

Abstract

In digitonin-permeabilized adrenal chromaffin cells, Ca2+-induced catecholamine release can be resolved into at least two sequential steps: a MgATP-dependent priming step and a MgATP-independent Ca2+-triggered step. Botulinum neurotoxins types A and E cleaved SNAP-25, and blocked MgATP-independent Ca2+-induced catecholamine release from the permeabilized chromaffin cells. When the permeabilized cells were primed by pretreatment with MgATP, the amount of SNAP-25 associated with VAMP-2 decreased, and the fraction of SNAP-25 proteolyzed by the neurotoxins increased. These results suggest that dissociation of SNAP-25 and VAMP-2 occurs during the MgATP-dependent priming step, and SNAP-25 plays some important roles in the subsequent MgATP-independent step.

Original languageEnglish
Pages (from-to)351-355
Number of pages5
JournalBrain Research
Volume737
Issue number1-2
DOIs
Publication statusPublished - Oct 21 1996

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Keywords

  • Botulinum neurotoxin
  • Exocytosis
  • MgATP
  • SNAP-24
  • VAMP-2

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology

Cite this

Misonou, H., Nishiki, T. I., Sekiguchi, M., Takahashi, M., Kamata, Y., Kozaki, S., Ohara-Imaizumi, M., & Kumakura, K. (1996). Dissociation of SNAP-25 and VAMP-2 by MgATP in permeabilized adrenal chromaffin cells. Brain Research, 737(1-2), 351-355. https://doi.org/10.1016/0006-8993(96)00954-7