TY - JOUR
T1 - Dissociation of SNAP-25 and VAMP-2 by MgATP in permeabilized adrenal chromaffin cells
AU - Misonou, Hiroaki
AU - Nishiki, Tei ichi
AU - Sekiguchi, Mariko
AU - Takahashi, Masami
AU - Kamata, Yoichi
AU - Kozaki, Shunji
AU - Ohara-Imaizumi, Mica
AU - Kumakura, Konosuke
PY - 1996/10/21
Y1 - 1996/10/21
N2 - In digitonin-permeabilized adrenal chromaffin cells, Ca2+-induced catecholamine release can be resolved into at least two sequential steps: a MgATP-dependent priming step and a MgATP-independent Ca2+-triggered step. Botulinum neurotoxins types A and E cleaved SNAP-25, and blocked MgATP-independent Ca2+-induced catecholamine release from the permeabilized chromaffin cells. When the permeabilized cells were primed by pretreatment with MgATP, the amount of SNAP-25 associated with VAMP-2 decreased, and the fraction of SNAP-25 proteolyzed by the neurotoxins increased. These results suggest that dissociation of SNAP-25 and VAMP-2 occurs during the MgATP-dependent priming step, and SNAP-25 plays some important roles in the subsequent MgATP-independent step.
AB - In digitonin-permeabilized adrenal chromaffin cells, Ca2+-induced catecholamine release can be resolved into at least two sequential steps: a MgATP-dependent priming step and a MgATP-independent Ca2+-triggered step. Botulinum neurotoxins types A and E cleaved SNAP-25, and blocked MgATP-independent Ca2+-induced catecholamine release from the permeabilized chromaffin cells. When the permeabilized cells were primed by pretreatment with MgATP, the amount of SNAP-25 associated with VAMP-2 decreased, and the fraction of SNAP-25 proteolyzed by the neurotoxins increased. These results suggest that dissociation of SNAP-25 and VAMP-2 occurs during the MgATP-dependent priming step, and SNAP-25 plays some important roles in the subsequent MgATP-independent step.
KW - Botulinum neurotoxin
KW - Exocytosis
KW - MgATP
KW - SNAP-24
KW - VAMP-2
UR - http://www.scopus.com/inward/record.url?scp=0030597143&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0030597143&partnerID=8YFLogxK
U2 - 10.1016/0006-8993(96)00954-7
DO - 10.1016/0006-8993(96)00954-7
M3 - Article
C2 - 8930391
AN - SCOPUS:0030597143
VL - 737
SP - 351
EP - 355
JO - Molecular Brain Research
JF - Molecular Brain Research
SN - 0006-8993
IS - 1-2
ER -