TY - JOUR
T1 - Dissociation of SNAP-25 and VAMP-2 by MgATP in permeabilized adrenal chromaffin cells
AU - Misonou, Hiroaki
AU - Nishiki, Tei ichi
AU - Sekiguchi, Mariko
AU - Takahashi, Masami
AU - Kamata, Yoichi
AU - Kozaki, Shunji
AU - Ohara-Imaizumi, Mica
AU - Kumakura, Konosuke
N1 - Funding Information:
We thank Dr. M.J. Seager for critical reading of the manuscript. This work was performed through Special Coordination Funds of the Science and Technology Agency of the Japanese Government of K.K., and supported in part by Grants-in-Aid for Scientific Research from the Ministry of Education, Science and Culture, Japan, to M.T.
PY - 1996/10/21
Y1 - 1996/10/21
N2 - In digitonin-permeabilized adrenal chromaffin cells, Ca2+-induced catecholamine release can be resolved into at least two sequential steps: a MgATP-dependent priming step and a MgATP-independent Ca2+-triggered step. Botulinum neurotoxins types A and E cleaved SNAP-25, and blocked MgATP-independent Ca2+-induced catecholamine release from the permeabilized chromaffin cells. When the permeabilized cells were primed by pretreatment with MgATP, the amount of SNAP-25 associated with VAMP-2 decreased, and the fraction of SNAP-25 proteolyzed by the neurotoxins increased. These results suggest that dissociation of SNAP-25 and VAMP-2 occurs during the MgATP-dependent priming step, and SNAP-25 plays some important roles in the subsequent MgATP-independent step.
AB - In digitonin-permeabilized adrenal chromaffin cells, Ca2+-induced catecholamine release can be resolved into at least two sequential steps: a MgATP-dependent priming step and a MgATP-independent Ca2+-triggered step. Botulinum neurotoxins types A and E cleaved SNAP-25, and blocked MgATP-independent Ca2+-induced catecholamine release from the permeabilized chromaffin cells. When the permeabilized cells were primed by pretreatment with MgATP, the amount of SNAP-25 associated with VAMP-2 decreased, and the fraction of SNAP-25 proteolyzed by the neurotoxins increased. These results suggest that dissociation of SNAP-25 and VAMP-2 occurs during the MgATP-dependent priming step, and SNAP-25 plays some important roles in the subsequent MgATP-independent step.
KW - Botulinum neurotoxin
KW - Exocytosis
KW - MgATP
KW - SNAP-24
KW - VAMP-2
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U2 - 10.1016/0006-8993(96)00954-7
DO - 10.1016/0006-8993(96)00954-7
M3 - Article
C2 - 8930391
AN - SCOPUS:0030597143
VL - 737
SP - 351
EP - 355
JO - Molecular Brain Research
JF - Molecular Brain Research
SN - 0006-8993
IS - 1-2
ER -