Abstract
The filamentous fungus Alternaria alternata contains seven pathogenic variants (pathotypes), which produce different host-specific toxins and cause diseases on different plants. The strawberry pathotype produces host-specific AF-toxin and causes Alternaria black spot of strawberry. This pathotype is also pathogenic to Japanese pear cultivars susceptible to the Japanese pear pathotype that produces AK-toxin. The strawberry pathotype produces two related molecular species, AF-toxins I and II: toxin I is toxic to both strawberry and pear, and toxin II is toxic only to pear. Previously, we isolated a cosmid clone pcAFT-1 from the strawberry pathotype that contains three genes involved in AF-toxin biosynthesis. Here, we have identified a new gene, designated AFTS1, from pcAFT-1. AFTS1 encodes a protein with similarity to enzymes of the aldo-ketoreductase superfamily. Targeted mutation of AFTS1 diminished the host range of the strawberry pathotype: ΔaftS1 mutants were pathogenic to pear, but not to strawberry, as is the Japanese pear pathotype. These mutants were found to produce AF-toxin II, but not AF-toxin I. These data represent a novel example of how the host range of a plant pathogenic fungus can be restricted by modification of secondary metabolism.
| Original language | English |
|---|---|
| Pages (from-to) | 399-411 |
| Number of pages | 13 |
| Journal | Molecular Microbiology |
| Volume | 52 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - Apr 2004 |
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ASJC Scopus subject areas
- Molecular Biology
- Microbiology
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Dissection of the host range of the fungal plant pathogen Alternaria alternata by modification of secondary metabolism. / Ito, Kaoru; Tanaka, Takayoshi; Hatta, Rieko; Yamamoto, Mikihiro; Akimitsu, Kazuya; Tsuge, Takashi.
In: Molecular Microbiology, Vol. 52, No. 2, 04.2004, p. 399-411.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Dissection of the host range of the fungal plant pathogen Alternaria alternata by modification of secondary metabolism
AU - Ito, Kaoru
AU - Tanaka, Takayoshi
AU - Hatta, Rieko
AU - Yamamoto, Mikihiro
AU - Akimitsu, Kazuya
AU - Tsuge, Takashi
PY - 2004/4
Y1 - 2004/4
N2 - The filamentous fungus Alternaria alternata contains seven pathogenic variants (pathotypes), which produce different host-specific toxins and cause diseases on different plants. The strawberry pathotype produces host-specific AF-toxin and causes Alternaria black spot of strawberry. This pathotype is also pathogenic to Japanese pear cultivars susceptible to the Japanese pear pathotype that produces AK-toxin. The strawberry pathotype produces two related molecular species, AF-toxins I and II: toxin I is toxic to both strawberry and pear, and toxin II is toxic only to pear. Previously, we isolated a cosmid clone pcAFT-1 from the strawberry pathotype that contains three genes involved in AF-toxin biosynthesis. Here, we have identified a new gene, designated AFTS1, from pcAFT-1. AFTS1 encodes a protein with similarity to enzymes of the aldo-ketoreductase superfamily. Targeted mutation of AFTS1 diminished the host range of the strawberry pathotype: ΔaftS1 mutants were pathogenic to pear, but not to strawberry, as is the Japanese pear pathotype. These mutants were found to produce AF-toxin II, but not AF-toxin I. These data represent a novel example of how the host range of a plant pathogenic fungus can be restricted by modification of secondary metabolism.
AB - The filamentous fungus Alternaria alternata contains seven pathogenic variants (pathotypes), which produce different host-specific toxins and cause diseases on different plants. The strawberry pathotype produces host-specific AF-toxin and causes Alternaria black spot of strawberry. This pathotype is also pathogenic to Japanese pear cultivars susceptible to the Japanese pear pathotype that produces AK-toxin. The strawberry pathotype produces two related molecular species, AF-toxins I and II: toxin I is toxic to both strawberry and pear, and toxin II is toxic only to pear. Previously, we isolated a cosmid clone pcAFT-1 from the strawberry pathotype that contains three genes involved in AF-toxin biosynthesis. Here, we have identified a new gene, designated AFTS1, from pcAFT-1. AFTS1 encodes a protein with similarity to enzymes of the aldo-ketoreductase superfamily. Targeted mutation of AFTS1 diminished the host range of the strawberry pathotype: ΔaftS1 mutants were pathogenic to pear, but not to strawberry, as is the Japanese pear pathotype. These mutants were found to produce AF-toxin II, but not AF-toxin I. These data represent a novel example of how the host range of a plant pathogenic fungus can be restricted by modification of secondary metabolism.
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UR - http://www.scopus.com/inward/citedby.url?scp=1942468805&partnerID=8YFLogxK
U2 - 10.1111/j.1365-2958.2004.04004.x
DO - 10.1111/j.1365-2958.2004.04004.x
M3 - Article
C2 - 15066029
AN - SCOPUS:1942468805
VL - 52
SP - 399
EP - 411
JO - Molecular Microbiology
JF - Molecular Microbiology
SN - 0950-382X
IS - 2
ER -