Disruption of ATM in p53-null cells causes multiple functional abnormalities in cellular response to ionizing radiation

Noriaki Takao, Hideaki Kato, Ryoichi Mori, Ciaran Morrison, Eiichiro Sonada, Xiango Sun, Hiroko Shimizu, Katsuji Yoshioka, Shunichi Takeda, Ken-ichi Yamamoto

Research output: Contribution to journalArticle

87 Citations (Scopus)

Abstract

ATM is a member of the large phosphatidylinositol-3 kinase family and plays an important role in cellular response to DNA damage. To further define the physiological roles of ATM at the cellular level, we created an isogenic set of stable cell lines differing only in their ATM status from the chicken B cell line DT40 by targeted integration. These stable DT40 cell lines, as most of transformed chicken cell lines, do not express p53. However, ATM(-/-) DT40 cells displayed retarded cellular proliferation, defective G2/M checkpoint control and radio-resistant DNA synthesis. Furthermore, ATM(-/-) DT40 cells were sensitive to ionizing radiation and showed highly elevated frequencies of both spontaneous and radiation-induced chromosomal aberrations. In addition, a slight but significant reduction in targeted integration frequency was observed in ATM(-/-) DT40 cells. These results suggest that ATM has multiple p53-independent functions in cell cycle checkpoint control and in maintenance of chromosomal DNA. These ATM deficient DT40 clones therefore provide a useful model system for analysing p53-independent ATM functions.

Original languageEnglish
Pages (from-to)7002-7009
Number of pages8
JournalOncogene
Volume18
Issue number50
Publication statusPublished - Nov 25 1999
Externally publishedYes

Fingerprint

Multiple Abnormalities
Null Lymphocytes
Ionizing Radiation
Cell Cycle Checkpoints
Cell Line
Chickens
Phosphatidylinositol 3-Kinase
Transformed Cell Line
DNA
Radio
Chromosome Aberrations
DNA Damage
B-Lymphocytes
Clone Cells
Maintenance
Cell Proliferation
Radiation

Keywords

  • ATM disruption
  • Cell cycle checkpoint
  • DNA repair
  • p53

ASJC Scopus subject areas

  • Molecular Biology
  • Cancer Research
  • Genetics

Cite this

Disruption of ATM in p53-null cells causes multiple functional abnormalities in cellular response to ionizing radiation. / Takao, Noriaki; Kato, Hideaki; Mori, Ryoichi; Morrison, Ciaran; Sonada, Eiichiro; Sun, Xiango; Shimizu, Hiroko; Yoshioka, Katsuji; Takeda, Shunichi; Yamamoto, Ken-ichi.

In: Oncogene, Vol. 18, No. 50, 25.11.1999, p. 7002-7009.

Research output: Contribution to journalArticle

Takao, N, Kato, H, Mori, R, Morrison, C, Sonada, E, Sun, X, Shimizu, H, Yoshioka, K, Takeda, S & Yamamoto, K 1999, 'Disruption of ATM in p53-null cells causes multiple functional abnormalities in cellular response to ionizing radiation', Oncogene, vol. 18, no. 50, pp. 7002-7009.
Takao N, Kato H, Mori R, Morrison C, Sonada E, Sun X et al. Disruption of ATM in p53-null cells causes multiple functional abnormalities in cellular response to ionizing radiation. Oncogene. 1999 Nov 25;18(50):7002-7009.
Takao, Noriaki ; Kato, Hideaki ; Mori, Ryoichi ; Morrison, Ciaran ; Sonada, Eiichiro ; Sun, Xiango ; Shimizu, Hiroko ; Yoshioka, Katsuji ; Takeda, Shunichi ; Yamamoto, Ken-ichi. / Disruption of ATM in p53-null cells causes multiple functional abnormalities in cellular response to ionizing radiation. In: Oncogene. 1999 ; Vol. 18, No. 50. pp. 7002-7009.
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