Disruption of a guard cell-expressed protein phosphatase 2A regulatory subunit, RCN1, confers abscisic acid insensitivity in arabidopsis

June M. Kwak, Ji Hye Moon, Yoshiyuki Murata, Kazuyuki Kuchitsu, Nathalie Leonhardt, Alison DeLong, Julian I. Schroeder

Research output: Contribution to journalArticle

159 Citations (Scopus)

Abstract

Pharmacological studies have led to a model in which the phytohormone abscisic acid (ABA) may be positively transduced via protein phosphatases of the type 1 (PP1) or type 2A (PP2A) families. However, pharmacological evidence also exists that PP1s or PP2As may function as negative regulators of ABA signaling. Furthermore, recessive disruption mutants in protein phosphatases that function in ABA signal transduction have not yet been identified. A guard cell-expressed PP2A gene, RCN1, which had been characterized previously as a molecular component affecting auxin transport and gravity response, was isolated. A T-DNA disruption mutation in RCN1 confers recessive ABA insensitivity to Arabidopsis. The rcn1 mutation impairs ABA-induced stomatal closing and ABA activation of slow anion channels. Calcium imaging analyses show a reduced sensitivity of ABA-induced cytosolic calcium increases in rcn1, whereas mechanisms downstream of cytosolic calcium increases show wild-type responses, suggesting that RCN1 functions in ABA signal transduction upstream of cytosolic Ca2+ increases. Furthermore, rcn1 shows ABA insensitivity in ABA inhibition of seed germination and ABA-induced gene expression. The PP1 and PP2A inhibitor okadaic acid phenocopies the rcn1 phenotype in wild-type plants both in ABA-induced cytosolic calcium increases and in seed germination, and the wild-type RCN1 genomic DNA complements rcn1 phenotypes. These data show that RCN1 functions as a general positive transducer of early ABA signaling.

Original languageEnglish
Pages (from-to)2849-2861
Number of pages13
JournalPlant Cell
Volume14
Issue number11
DOIs
Publication statusPublished - Nov 1 2002
Externally publishedYes

Fingerprint

Protein Phosphatase 2
phosphoprotein phosphatase
Abscisic Acid
guard cells
Arabidopsis
abscisic acid
calcium
Calcium
Protein Phosphatase 1
Signal transduction
Germination
Seed
signal transduction
Signal Transduction
Seeds
seed germination
Pharmacology
Phenotype
mutation
phenotype

ASJC Scopus subject areas

  • Plant Science
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

Cite this

Disruption of a guard cell-expressed protein phosphatase 2A regulatory subunit, RCN1, confers abscisic acid insensitivity in arabidopsis. / Kwak, June M.; Moon, Ji Hye; Murata, Yoshiyuki; Kuchitsu, Kazuyuki; Leonhardt, Nathalie; DeLong, Alison; Schroeder, Julian I.

In: Plant Cell, Vol. 14, No. 11, 01.11.2002, p. 2849-2861.

Research output: Contribution to journalArticle

Kwak, June M. ; Moon, Ji Hye ; Murata, Yoshiyuki ; Kuchitsu, Kazuyuki ; Leonhardt, Nathalie ; DeLong, Alison ; Schroeder, Julian I. / Disruption of a guard cell-expressed protein phosphatase 2A regulatory subunit, RCN1, confers abscisic acid insensitivity in arabidopsis. In: Plant Cell. 2002 ; Vol. 14, No. 11. pp. 2849-2861.
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