Disruption of a dna topoisomerase I gene affects morphogenesis in arabidopsis

Taku Takahashi, Shio Matsuhara, Mitsutomo Abe, Yoshibumi Komeda

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

The genesis of phyllotaxis, which often is associated with the Fibonacci series of numbers, is an old unsolved puzzle in plant morphogenesis. Here, we show that disruption of an Arabidopsis topoisomerase (topo) I gene named TOP1α affects phyllotaxis and plant architecture. The divergence angles and internode lengths between two successive flowers were more random in the top1α mutant than in the wild type. The top1α plants sporadically produced multiple flowers from one node, and the number of floral organ primordia often was different. The mutation also caused the twisting of inflorescences and individual flowers and the serration of leaf margins. These morphological abnormalities indicate that TOP1α may play a critical role in the maintenance of a regular pattern of organ initiation. The top1α mutant transformed with the RNA interference construct for TOP1β, another topo I gene arrayed tandemly with TOP1α, was found to be lethal at young seedling stages, suggesting that topo I activity is essential in plants.

Original languageEnglish
Pages (from-to)2085-2093
Number of pages9
JournalPlant Cell
Volume14
Issue number9
DOIs
Publication statusPublished - Sep 2002
Externally publishedYes

Fingerprint

DNA topoisomerase
Type I DNA Topoisomerase
Morphogenesis
Arabidopsis
phyllotaxy
morphogenesis
Genes
flowers
Inflorescence
mutants
Plant Development
genes
plant architecture
RNA Interference
RNA interference
lethal genes
Seedlings
internodes
inflorescences
Maintenance

ASJC Scopus subject areas

  • Plant Science
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

Cite this

Disruption of a dna topoisomerase I gene affects morphogenesis in arabidopsis. / Takahashi, Taku; Matsuhara, Shio; Abe, Mitsutomo; Komeda, Yoshibumi.

In: Plant Cell, Vol. 14, No. 9, 09.2002, p. 2085-2093.

Research output: Contribution to journalArticle

Takahashi, Taku ; Matsuhara, Shio ; Abe, Mitsutomo ; Komeda, Yoshibumi. / Disruption of a dna topoisomerase I gene affects morphogenesis in arabidopsis. In: Plant Cell. 2002 ; Vol. 14, No. 9. pp. 2085-2093.
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