Disposition of diadenosine 5',5'''-P1,P4-tetraphosphate (Ap4A) in rats

T. Kimura, N. Hatano, M. Wada, K. Iwata, Yuji Kurosaki, T. Nakayama, T. Yamaura, H. Nakajima

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The disposition of diadenosine 5',5'''-P1,P4-tetraphosphate (Ap4A), an endogenous dinucleotide, was investigated in rats. The degradation of Ap4A in rat plasma was very rapid and could be explained by a Michaelis-Menten equation: K(m) and V(max) values were 1.69 μg/ml and 4.32 μg/min/ml, respectively. Ap4A was degraded in rat plasma to ATP and AMP, but not to 2 ADP molecules, and these nucleotides were further degraded through adenosine. The degradation kinetics were examined. After intravenous bolus injection, Ap4A in plasma declined rapidly and the rate of elimination was dose- dependent: the biological half-life was about 3 s at the dose of 1 mg/kg and was longer at 3 mg/kg. When Ap4A was administered by intravenous infusion (0.5 or 1.0 mg/kg/min), the plasma level rapidly reached a steady-state, which then rapidly declined after stopping the infusion.

Original languageEnglish
Pages (from-to)1556-1559
Number of pages4
JournalBiological and Pharmaceutical Bulletin
Volume18
Issue number11
Publication statusPublished - 1995

Fingerprint

Adenosine Monophosphate
Intravenous Infusions
Intravenous Injections
Adenosine
Adenosine Diphosphate
Half-Life
Nucleotides
Adenosine Triphosphate
diadenosine tetraphosphate

Keywords

  • Ap4A
  • diadenosine 5',5'''-P,P-tetraphosphate
  • kinetical analysis
  • plasma degradation
  • rat

ASJC Scopus subject areas

  • Molecular Medicine
  • Pharmacology, Toxicology and Pharmaceutics(all)

Cite this

Kimura, T., Hatano, N., Wada, M., Iwata, K., Kurosaki, Y., Nakayama, T., ... Nakajima, H. (1995). Disposition of diadenosine 5',5'''-P1,P4-tetraphosphate (Ap4A) in rats. Biological and Pharmaceutical Bulletin, 18(11), 1556-1559.

Disposition of diadenosine 5',5'''-P1,P4-tetraphosphate (Ap4A) in rats. / Kimura, T.; Hatano, N.; Wada, M.; Iwata, K.; Kurosaki, Yuji; Nakayama, T.; Yamaura, T.; Nakajima, H.

In: Biological and Pharmaceutical Bulletin, Vol. 18, No. 11, 1995, p. 1556-1559.

Research output: Contribution to journalArticle

Kimura, T, Hatano, N, Wada, M, Iwata, K, Kurosaki, Y, Nakayama, T, Yamaura, T & Nakajima, H 1995, 'Disposition of diadenosine 5',5'''-P1,P4-tetraphosphate (Ap4A) in rats', Biological and Pharmaceutical Bulletin, vol. 18, no. 11, pp. 1556-1559.
Kimura T, Hatano N, Wada M, Iwata K, Kurosaki Y, Nakayama T et al. Disposition of diadenosine 5',5'''-P1,P4-tetraphosphate (Ap4A) in rats. Biological and Pharmaceutical Bulletin. 1995;18(11):1556-1559.
Kimura, T. ; Hatano, N. ; Wada, M. ; Iwata, K. ; Kurosaki, Yuji ; Nakayama, T. ; Yamaura, T. ; Nakajima, H. / Disposition of diadenosine 5',5'''-P1,P4-tetraphosphate (Ap4A) in rats. In: Biological and Pharmaceutical Bulletin. 1995 ; Vol. 18, No. 11. pp. 1556-1559.
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