Dispersive liquid–liquid microextraction coupled with microfluidic paper-based analytical device for the determination of organophosphate and carbamate pesticides in the water sample

Sheleme Beshana, Ahmed Hussen, Seyoum Leta, Takashi Kaneta

Research output: Contribution to journalArticlepeer-review

Abstract

Abstract: A microfluidic paper-based analytical device (µ-PAD) is a promising new technology platform for the development of extremely low-cost sensing devices. However, it has low sensitivity that might not enable to measure maximum allowable concentration of various pollutants in the environment. In this study, a dispersive liquid–liquid microextraction (DLLME) was developed as a preconcentration method to enhance the sensitivity of the µ-PAD for trace analysis of selected pesticides. Four critical parameters (volume of n-hexane and acetone, extraction time, NaCl amount) that affect the efficiency of DLLME have been optimized using response surface methodology. An acceptable mean recovery of 79–97% and 83–93% was observed at 1 µg L−1 and 5 µg L−1 fortification level, respectively, with very good repeatability (2.2–6.01% RSD) and reproducibility (5.60–10.41% RSD). Very high enrichment factors ranging from 317 to 1471 were obtained. The limits of detection for the studied analytes were in the range of 0.18–0.41 µg L−1 which is much lower than the WHO limits of 5–50 µg L−1 for similar category of analytes. Therefore, by coupling DLLME with µ-PAD, a sensitivity that allows to detect environmental threat and also that surpassed most of the previous reports have been achieved in this study. This implies that the preconcentration step has a paramount contribution to address the sensitivity problem associated with µ-PAD. Graphical abstract: [Figure not available: see fulltext.]

Original languageEnglish
Pages (from-to)1359-1367
Number of pages9
JournalAnalytical Sciences
Volume38
Issue number10
DOIs
Publication statusPublished - Oct 2022

Keywords

  • Box–Behnken design
  • Dispersive liquid–liquid microextraction
  • Inhibition percent
  • Microfluidic paper-based analytical device
  • Pesticide

ASJC Scopus subject areas

  • Analytical Chemistry

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