Abstract
A bio array sensing system is newly composed utilizing different phospholipid liposomes encapsulating fluorescent molecules. We have confirmed a high output intensity of fluorescence emission due to the characteristics dependent on the concentration of fluorescent molecules when the fluorescent molecules leak from inside of the liposome through perturbed lipid membrane. After measuring a whole array image of fluorescence emission output from every element of liposome sensor by a new CMOS imager system, the outputs of fluorescence emission from all the elements were analyzed by a statistical method of principal component analysis (PCA). It was found from obtained PCA plots that different species of proteins with several concentrations were discriminated with high cumulative contribution ratio.
| Original language | English |
|---|---|
| Title of host publication | 2015 IEEE SENSORS - Proceedings |
| Publisher | Institute of Electrical and Electronics Engineers Inc. |
| ISBN (Print) | 9781479982028 |
| DOIs | |
| Publication status | Published - Dec 31 2015 |
| Event | 14th IEEE SENSORS - Busan, Korea, Republic of Duration: Nov 1 2015 → Nov 4 2015 |
Other
| Other | 14th IEEE SENSORS |
|---|---|
| Country | Korea, Republic of |
| City | Busan |
| Period | 11/1/15 → 11/4/15 |
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Keywords
- Array
- Biosensor
- Cholesterol
- Fluorescence
- Liposome
- Principal Component Analysis
- Protein
ASJC Scopus subject areas
- Instrumentation
- Electronic, Optical and Magnetic Materials
- Spectroscopy
- Electrical and Electronic Engineering
Cite this
Discrimination of target proteins using arrayed fluorescent liposomes incorporated with cholesterol by principal component analysis. / Imamura, R.; Zhang, Z.; Yoshikawa, T.; Murata, N.; Yamashita, K.; Fukuzawa, M.; Noda, M.; Shimanouchi, Toshinori.
2015 IEEE SENSORS - Proceedings. Institute of Electrical and Electronics Engineers Inc., 2015. 7370493.Research output: Chapter in Book/Report/Conference proceeding › Conference contribution
}
TY - GEN
T1 - Discrimination of target proteins using arrayed fluorescent liposomes incorporated with cholesterol by principal component analysis
AU - Imamura, R.
AU - Zhang, Z.
AU - Yoshikawa, T.
AU - Murata, N.
AU - Yamashita, K.
AU - Fukuzawa, M.
AU - Noda, M.
AU - Shimanouchi, Toshinori
PY - 2015/12/31
Y1 - 2015/12/31
N2 - A bio array sensing system is newly composed utilizing different phospholipid liposomes encapsulating fluorescent molecules. We have confirmed a high output intensity of fluorescence emission due to the characteristics dependent on the concentration of fluorescent molecules when the fluorescent molecules leak from inside of the liposome through perturbed lipid membrane. After measuring a whole array image of fluorescence emission output from every element of liposome sensor by a new CMOS imager system, the outputs of fluorescence emission from all the elements were analyzed by a statistical method of principal component analysis (PCA). It was found from obtained PCA plots that different species of proteins with several concentrations were discriminated with high cumulative contribution ratio.
AB - A bio array sensing system is newly composed utilizing different phospholipid liposomes encapsulating fluorescent molecules. We have confirmed a high output intensity of fluorescence emission due to the characteristics dependent on the concentration of fluorescent molecules when the fluorescent molecules leak from inside of the liposome through perturbed lipid membrane. After measuring a whole array image of fluorescence emission output from every element of liposome sensor by a new CMOS imager system, the outputs of fluorescence emission from all the elements were analyzed by a statistical method of principal component analysis (PCA). It was found from obtained PCA plots that different species of proteins with several concentrations were discriminated with high cumulative contribution ratio.
KW - Array
KW - Biosensor
KW - Cholesterol
KW - Fluorescence
KW - Liposome
KW - Principal Component Analysis
KW - Protein
UR - http://www.scopus.com/inward/record.url?scp=84963607555&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84963607555&partnerID=8YFLogxK
U2 - 10.1109/ICSENS.2015.7370493
DO - 10.1109/ICSENS.2015.7370493
M3 - Conference contribution
AN - SCOPUS:84963607555
SN - 9781479982028
BT - 2015 IEEE SENSORS - Proceedings
PB - Institute of Electrical and Electronics Engineers Inc.
ER -