Discrimination of target proteins using arrayed fluorescent liposomes incorporated with cholesterol by principal component analysis

R. Imamura; Z. Zhang; T. Yoshikawa; N. Murata; K. Yamashita; M. Fukuzawa; M. Noda; Toshinori Shimanouchi

doi:10.1109/ICSENS.2015.7370493

Discrimination of target proteins using arrayed fluorescent liposomes incorporated with cholesterol by principal component analysis

R. Imamura, Z. Zhang, T. Yoshikawa, N. Murata, K. Yamashita, M. Fukuzawa, M. Noda, Toshinori Shimanouchi

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

2 Citations (Scopus)

Abstract

A bio array sensing system is newly composed utilizing different phospholipid liposomes encapsulating fluorescent molecules. We have confirmed a high output intensity of fluorescence emission due to the characteristics dependent on the concentration of fluorescent molecules when the fluorescent molecules leak from inside of the liposome through perturbed lipid membrane. After measuring a whole array image of fluorescence emission output from every element of liposome sensor by a new CMOS imager system, the outputs of fluorescence emission from all the elements were analyzed by a statistical method of principal component analysis (PCA). It was found from obtained PCA plots that different species of proteins with several concentrations were discriminated with high cumulative contribution ratio.

Original language	English
Title of host publication	2015 IEEE SENSORS - Proceedings
Publisher	Institute of Electrical and Electronics Engineers Inc.
ISBN (Print)	9781479982028
DOIs	https://doi.org/10.1109/ICSENS.2015.7370493
Publication status	Published - Dec 31 2015
Event	14th IEEE SENSORS - Busan, Korea, Republic of Duration: Nov 1 2015 → Nov 4 2015

Other

Other	14th IEEE SENSORS
Country	Korea, Republic of
City	Busan
Period	11/1/15 → 11/4/15

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Keywords

Array
Biosensor
Cholesterol
Fluorescence
Liposome
Principal Component Analysis
Protein

ASJC Scopus subject areas

Instrumentation
Electronic, Optical and Magnetic Materials
Spectroscopy
Electrical and Electronic Engineering

Cite this

Imamura, R., Zhang, Z., Yoshikawa, T., Murata, N., Yamashita, K., Fukuzawa, M., Noda, M., & Shimanouchi, T. (2015). Discrimination of target proteins using arrayed fluorescent liposomes incorporated with cholesterol by principal component analysis. In 2015 IEEE SENSORS - Proceedings [7370493] Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/ICSENS.2015.7370493

Discrimination of target proteins using arrayed fluorescent liposomes incorporated with cholesterol by principal component analysis. / Imamura, R.; Zhang, Z.; Yoshikawa, T.; Murata, N.; Yamashita, K.; Fukuzawa, M.; Noda, M.; Shimanouchi, Toshinori.

2015 IEEE SENSORS - Proceedings. Institute of Electrical and Electronics Engineers Inc., 2015. 7370493.

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Imamura, R, Zhang, Z, Yoshikawa, T, Murata, N, Yamashita, K, Fukuzawa, M, Noda, M & Shimanouchi, T 2015, Discrimination of target proteins using arrayed fluorescent liposomes incorporated with cholesterol by principal component analysis. in 2015 IEEE SENSORS - Proceedings., 7370493, Institute of Electrical and Electronics Engineers Inc., 14th IEEE SENSORS, Busan, Korea, Republic of, 11/1/15. https://doi.org/10.1109/ICSENS.2015.7370493

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Access to Document

10.1109/ICSENS.2015.7370493