Discovery of genes related to diabetic nephropathy in various animal models by current techniques

Jun Wada, Lin Sun, Yashpal S. Kanwar

Research output: Chapter in Book/Report/Conference proceedingChapter

13 Citations (Scopus)

Abstract

One of the major problems facing clinical nephrology currently throughout the world is an exponential increase in patients with end-stage renal disease (ESRD), which is largely related to a high incidence of diabetic nephropathy. The latter is characterized by a multitude of metabolic and signaling events following excessive channeling of glucose, which leads to an increased synthesis of extracellular matrix (ECM) glycoproteins resulting in glomerulosclerosis, interstitial fibrosis and ultimately ESRD. With the incidence of nephropathy at pandemic levels and a high rate of ESRD, physicians around the world must treat a disproportionately large number of diabetic patients with upto-date innovative measures. In this regard, identification of genes that are crucially involved in the progression of diabetic nephropathy would enhance the discovery of new biomarkers and could also promote the development of novel therapeutic strategies. Over the last decade, we focused on the recent methodologies of high-throughput and genome-wide screening for identification of relevant genes in various animal models, which included the following: (1) single nucleotide polymorphism-based genome-wide screening; (2) the transcriptome approach, such as differential display reverse transcription polymerase chain reaction (DDRT-PCR), representational difference analysis of cDNA (cDNA-RDA)/suppressive subtractive hybridization, SAGE (serial analysis of gene expression) and DNA Microarray; and (3) the proteomic approach and 2-dimensional polyacrylamide gel electrophoresis (2D-PAGE) coupled with mass spectroscopic analysis. Several genes, such as Tim44 (translocase of inner mitochondrial membrane-44), RSOR/MIOX (renal specific oxidoreductase/myo-inositol oxygenase), UbA52, Rap1b (Ras-related GTPase), gremlin, osteopontin, hydroxysteroid dehydrogenase-3β isotype 4 and those of the Wnt signaling pathway, were identified as differentially expressed genes in kidneys of diabetic rodents. Functional analysis of these genes and the subsequent translational research in the clinical settings would be very valuable in the prevention and treatment of diabetic nephropathy. Future trends for identification of the biomarkers and therapeutic target genes should also include genome scale DNA/histonemethylation profiling, metabolomic approaches (e.g. metabolic phenotyping by 1H spectroscopy) and lectin microarray for glycan profiling along with the development of robust data-mining strategies.

Original languageEnglish
Title of host publicationExperimental Models for Renal Diseases: Pathogenesis and Diagnosis
PublisherS. Karger AG
Pages161-174
Number of pages14
Volume169
ISBN (Print)9783805595384, 9783805595377
DOIs
Publication statusPublished - Jan 14 2011

Fingerprint

Diabetic Nephropathies
Genetic Association Studies
Animals
Animal Models
Genes
Chronic Kidney Failure
Genome
Inositol Oxygenase
Complementary DNA
3-Hydroxysteroid Dehydrogenases
Biomarkers
Microarrays
Kidney
Wnt Signaling Pathway
Osteopontin
Translational Medical Research
Metabolomics
Data Mining
Nephrology
Monomeric GTP-Binding Proteins

ASJC Scopus subject areas

  • Medicine(all)
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Wada, J., Sun, L., & Kanwar, Y. S. (2011). Discovery of genes related to diabetic nephropathy in various animal models by current techniques. In Experimental Models for Renal Diseases: Pathogenesis and Diagnosis (Vol. 169, pp. 161-174). S. Karger AG. https://doi.org/10.1159/000313951

Discovery of genes related to diabetic nephropathy in various animal models by current techniques. / Wada, Jun; Sun, Lin; Kanwar, Yashpal S.

Experimental Models for Renal Diseases: Pathogenesis and Diagnosis. Vol. 169 S. Karger AG, 2011. p. 161-174.

Research output: Chapter in Book/Report/Conference proceedingChapter

Wada, J, Sun, L & Kanwar, YS 2011, Discovery of genes related to diabetic nephropathy in various animal models by current techniques. in Experimental Models for Renal Diseases: Pathogenesis and Diagnosis. vol. 169, S. Karger AG, pp. 161-174. https://doi.org/10.1159/000313951
Wada J, Sun L, Kanwar YS. Discovery of genes related to diabetic nephropathy in various animal models by current techniques. In Experimental Models for Renal Diseases: Pathogenesis and Diagnosis. Vol. 169. S. Karger AG. 2011. p. 161-174 https://doi.org/10.1159/000313951
Wada, Jun ; Sun, Lin ; Kanwar, Yashpal S. / Discovery of genes related to diabetic nephropathy in various animal models by current techniques. Experimental Models for Renal Diseases: Pathogenesis and Diagnosis. Vol. 169 S. Karger AG, 2011. pp. 161-174
@inbook{1d5c5d8e10324050b3329c15de22bbe9,
title = "Discovery of genes related to diabetic nephropathy in various animal models by current techniques",
abstract = "One of the major problems facing clinical nephrology currently throughout the world is an exponential increase in patients with end-stage renal disease (ESRD), which is largely related to a high incidence of diabetic nephropathy. The latter is characterized by a multitude of metabolic and signaling events following excessive channeling of glucose, which leads to an increased synthesis of extracellular matrix (ECM) glycoproteins resulting in glomerulosclerosis, interstitial fibrosis and ultimately ESRD. With the incidence of nephropathy at pandemic levels and a high rate of ESRD, physicians around the world must treat a disproportionately large number of diabetic patients with upto-date innovative measures. In this regard, identification of genes that are crucially involved in the progression of diabetic nephropathy would enhance the discovery of new biomarkers and could also promote the development of novel therapeutic strategies. Over the last decade, we focused on the recent methodologies of high-throughput and genome-wide screening for identification of relevant genes in various animal models, which included the following: (1) single nucleotide polymorphism-based genome-wide screening; (2) the transcriptome approach, such as differential display reverse transcription polymerase chain reaction (DDRT-PCR), representational difference analysis of cDNA (cDNA-RDA)/suppressive subtractive hybridization, SAGE (serial analysis of gene expression) and DNA Microarray; and (3) the proteomic approach and 2-dimensional polyacrylamide gel electrophoresis (2D-PAGE) coupled with mass spectroscopic analysis. Several genes, such as Tim44 (translocase of inner mitochondrial membrane-44), RSOR/MIOX (renal specific oxidoreductase/myo-inositol oxygenase), UbA52, Rap1b (Ras-related GTPase), gremlin, osteopontin, hydroxysteroid dehydrogenase-3β isotype 4 and those of the Wnt signaling pathway, were identified as differentially expressed genes in kidneys of diabetic rodents. Functional analysis of these genes and the subsequent translational research in the clinical settings would be very valuable in the prevention and treatment of diabetic nephropathy. Future trends for identification of the biomarkers and therapeutic target genes should also include genome scale DNA/histonemethylation profiling, metabolomic approaches (e.g. metabolic phenotyping by 1H spectroscopy) and lectin microarray for glycan profiling along with the development of robust data-mining strategies.",
author = "Jun Wada and Lin Sun and Kanwar, {Yashpal S.}",
year = "2011",
month = "1",
day = "14",
doi = "10.1159/000313951",
language = "English",
isbn = "9783805595384",
volume = "169",
pages = "161--174",
booktitle = "Experimental Models for Renal Diseases: Pathogenesis and Diagnosis",
publisher = "S. Karger AG",

}

TY - CHAP

T1 - Discovery of genes related to diabetic nephropathy in various animal models by current techniques

AU - Wada, Jun

AU - Sun, Lin

AU - Kanwar, Yashpal S.

PY - 2011/1/14

Y1 - 2011/1/14

N2 - One of the major problems facing clinical nephrology currently throughout the world is an exponential increase in patients with end-stage renal disease (ESRD), which is largely related to a high incidence of diabetic nephropathy. The latter is characterized by a multitude of metabolic and signaling events following excessive channeling of glucose, which leads to an increased synthesis of extracellular matrix (ECM) glycoproteins resulting in glomerulosclerosis, interstitial fibrosis and ultimately ESRD. With the incidence of nephropathy at pandemic levels and a high rate of ESRD, physicians around the world must treat a disproportionately large number of diabetic patients with upto-date innovative measures. In this regard, identification of genes that are crucially involved in the progression of diabetic nephropathy would enhance the discovery of new biomarkers and could also promote the development of novel therapeutic strategies. Over the last decade, we focused on the recent methodologies of high-throughput and genome-wide screening for identification of relevant genes in various animal models, which included the following: (1) single nucleotide polymorphism-based genome-wide screening; (2) the transcriptome approach, such as differential display reverse transcription polymerase chain reaction (DDRT-PCR), representational difference analysis of cDNA (cDNA-RDA)/suppressive subtractive hybridization, SAGE (serial analysis of gene expression) and DNA Microarray; and (3) the proteomic approach and 2-dimensional polyacrylamide gel electrophoresis (2D-PAGE) coupled with mass spectroscopic analysis. Several genes, such as Tim44 (translocase of inner mitochondrial membrane-44), RSOR/MIOX (renal specific oxidoreductase/myo-inositol oxygenase), UbA52, Rap1b (Ras-related GTPase), gremlin, osteopontin, hydroxysteroid dehydrogenase-3β isotype 4 and those of the Wnt signaling pathway, were identified as differentially expressed genes in kidneys of diabetic rodents. Functional analysis of these genes and the subsequent translational research in the clinical settings would be very valuable in the prevention and treatment of diabetic nephropathy. Future trends for identification of the biomarkers and therapeutic target genes should also include genome scale DNA/histonemethylation profiling, metabolomic approaches (e.g. metabolic phenotyping by 1H spectroscopy) and lectin microarray for glycan profiling along with the development of robust data-mining strategies.

AB - One of the major problems facing clinical nephrology currently throughout the world is an exponential increase in patients with end-stage renal disease (ESRD), which is largely related to a high incidence of diabetic nephropathy. The latter is characterized by a multitude of metabolic and signaling events following excessive channeling of glucose, which leads to an increased synthesis of extracellular matrix (ECM) glycoproteins resulting in glomerulosclerosis, interstitial fibrosis and ultimately ESRD. With the incidence of nephropathy at pandemic levels and a high rate of ESRD, physicians around the world must treat a disproportionately large number of diabetic patients with upto-date innovative measures. In this regard, identification of genes that are crucially involved in the progression of diabetic nephropathy would enhance the discovery of new biomarkers and could also promote the development of novel therapeutic strategies. Over the last decade, we focused on the recent methodologies of high-throughput and genome-wide screening for identification of relevant genes in various animal models, which included the following: (1) single nucleotide polymorphism-based genome-wide screening; (2) the transcriptome approach, such as differential display reverse transcription polymerase chain reaction (DDRT-PCR), representational difference analysis of cDNA (cDNA-RDA)/suppressive subtractive hybridization, SAGE (serial analysis of gene expression) and DNA Microarray; and (3) the proteomic approach and 2-dimensional polyacrylamide gel electrophoresis (2D-PAGE) coupled with mass spectroscopic analysis. Several genes, such as Tim44 (translocase of inner mitochondrial membrane-44), RSOR/MIOX (renal specific oxidoreductase/myo-inositol oxygenase), UbA52, Rap1b (Ras-related GTPase), gremlin, osteopontin, hydroxysteroid dehydrogenase-3β isotype 4 and those of the Wnt signaling pathway, were identified as differentially expressed genes in kidneys of diabetic rodents. Functional analysis of these genes and the subsequent translational research in the clinical settings would be very valuable in the prevention and treatment of diabetic nephropathy. Future trends for identification of the biomarkers and therapeutic target genes should also include genome scale DNA/histonemethylation profiling, metabolomic approaches (e.g. metabolic phenotyping by 1H spectroscopy) and lectin microarray for glycan profiling along with the development of robust data-mining strategies.

UR - http://www.scopus.com/inward/record.url?scp=84925937379&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84925937379&partnerID=8YFLogxK

U2 - 10.1159/000313951

DO - 10.1159/000313951

M3 - Chapter

C2 - 21252517

AN - SCOPUS:79551484955

SN - 9783805595384

SN - 9783805595377

VL - 169

SP - 161

EP - 174

BT - Experimental Models for Renal Diseases: Pathogenesis and Diagnosis

PB - S. Karger AG

ER -