DISC1 localizes to the centrosome by binding to kendrin

Ko Miyoshi, Masato Asanuma, Ikuko Miyazaki, Francisco J. Diaz-Corrales, Taiichi Katayama, Masaya Tohyama, Norio Ogawa

Research output: Contribution to journalArticle

85 Citations (Scopus)

Abstract

Disrupted-In-Schizophrenia 1 (DISC1) was identified as a novel gene disrupted by a (1;11)(q42.1;q14.3) translocation that segregated with major mental disorders in a Scottish family. Using the yeast two-hybrid system, we screened a human brain cDNA library for interactors of the DISC1 protein. One of the positive clones encoded kendrin/pericentrin-B, a giant protein known to localize specifically to the centrosome. The interaction between DISC1 and kendrin in mammalian cells was demonstrated by an immunoprecipitation assay. Residues 446-533 of DISC1 were essential for the interaction with kendrin. Immunocytochemical analysis revealed the colocalization of DISC1 and kendrin to the centrosome. These data indicate that DISC1 localizes to the centrosome by binding to kendrin. Kendrin has been reported to anchor the γ-tubulin complex to the centrosome, providing microtubule nucleation sites. The present study suggests the possible involvement of DISC1 in the pathophysiology of mental disorders due to its putative effect on centrosomal function.

Original languageEnglish
Pages (from-to)1195-1199
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume317
Issue number4
DOIs
Publication statusPublished - May 14 2004

Fingerprint

Centrosome
Schizophrenia
Mental Disorders
Two-Hybrid System Techniques
Tubulin
Anchors
Hybrid systems
Gene Library
Yeast
kendrin
Assays
Immunoprecipitation
Brain
Microtubules
Proteins
Nucleation
Genes
Cells
Clone Cells

Keywords

  • Affective disorders
  • Centrosome
  • DISC1
  • Kendrin
  • Mental disorders
  • Pericentrin
  • Schizophrenia

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

DISC1 localizes to the centrosome by binding to kendrin. / Miyoshi, Ko; Asanuma, Masato; Miyazaki, Ikuko; Diaz-Corrales, Francisco J.; Katayama, Taiichi; Tohyama, Masaya; Ogawa, Norio.

In: Biochemical and Biophysical Research Communications, Vol. 317, No. 4, 14.05.2004, p. 1195-1199.

Research output: Contribution to journalArticle

Miyoshi, Ko ; Asanuma, Masato ; Miyazaki, Ikuko ; Diaz-Corrales, Francisco J. ; Katayama, Taiichi ; Tohyama, Masaya ; Ogawa, Norio. / DISC1 localizes to the centrosome by binding to kendrin. In: Biochemical and Biophysical Research Communications. 2004 ; Vol. 317, No. 4. pp. 1195-1199.
@article{ad8f5736a6f3402daba9eb7cf31dcd83,
title = "DISC1 localizes to the centrosome by binding to kendrin",
abstract = "Disrupted-In-Schizophrenia 1 (DISC1) was identified as a novel gene disrupted by a (1;11)(q42.1;q14.3) translocation that segregated with major mental disorders in a Scottish family. Using the yeast two-hybrid system, we screened a human brain cDNA library for interactors of the DISC1 protein. One of the positive clones encoded kendrin/pericentrin-B, a giant protein known to localize specifically to the centrosome. The interaction between DISC1 and kendrin in mammalian cells was demonstrated by an immunoprecipitation assay. Residues 446-533 of DISC1 were essential for the interaction with kendrin. Immunocytochemical analysis revealed the colocalization of DISC1 and kendrin to the centrosome. These data indicate that DISC1 localizes to the centrosome by binding to kendrin. Kendrin has been reported to anchor the γ-tubulin complex to the centrosome, providing microtubule nucleation sites. The present study suggests the possible involvement of DISC1 in the pathophysiology of mental disorders due to its putative effect on centrosomal function.",
keywords = "Affective disorders, Centrosome, DISC1, Kendrin, Mental disorders, Pericentrin, Schizophrenia",
author = "Ko Miyoshi and Masato Asanuma and Ikuko Miyazaki and Diaz-Corrales, {Francisco J.} and Taiichi Katayama and Masaya Tohyama and Norio Ogawa",
year = "2004",
month = "5",
day = "14",
doi = "10.1016/j.bbrc.2004.03.163",
language = "English",
volume = "317",
pages = "1195--1199",
journal = "Biochemical and Biophysical Research Communications",
issn = "0006-291X",
publisher = "Academic Press Inc.",
number = "4",

}

TY - JOUR

T1 - DISC1 localizes to the centrosome by binding to kendrin

AU - Miyoshi, Ko

AU - Asanuma, Masato

AU - Miyazaki, Ikuko

AU - Diaz-Corrales, Francisco J.

AU - Katayama, Taiichi

AU - Tohyama, Masaya

AU - Ogawa, Norio

PY - 2004/5/14

Y1 - 2004/5/14

N2 - Disrupted-In-Schizophrenia 1 (DISC1) was identified as a novel gene disrupted by a (1;11)(q42.1;q14.3) translocation that segregated with major mental disorders in a Scottish family. Using the yeast two-hybrid system, we screened a human brain cDNA library for interactors of the DISC1 protein. One of the positive clones encoded kendrin/pericentrin-B, a giant protein known to localize specifically to the centrosome. The interaction between DISC1 and kendrin in mammalian cells was demonstrated by an immunoprecipitation assay. Residues 446-533 of DISC1 were essential for the interaction with kendrin. Immunocytochemical analysis revealed the colocalization of DISC1 and kendrin to the centrosome. These data indicate that DISC1 localizes to the centrosome by binding to kendrin. Kendrin has been reported to anchor the γ-tubulin complex to the centrosome, providing microtubule nucleation sites. The present study suggests the possible involvement of DISC1 in the pathophysiology of mental disorders due to its putative effect on centrosomal function.

AB - Disrupted-In-Schizophrenia 1 (DISC1) was identified as a novel gene disrupted by a (1;11)(q42.1;q14.3) translocation that segregated with major mental disorders in a Scottish family. Using the yeast two-hybrid system, we screened a human brain cDNA library for interactors of the DISC1 protein. One of the positive clones encoded kendrin/pericentrin-B, a giant protein known to localize specifically to the centrosome. The interaction between DISC1 and kendrin in mammalian cells was demonstrated by an immunoprecipitation assay. Residues 446-533 of DISC1 were essential for the interaction with kendrin. Immunocytochemical analysis revealed the colocalization of DISC1 and kendrin to the centrosome. These data indicate that DISC1 localizes to the centrosome by binding to kendrin. Kendrin has been reported to anchor the γ-tubulin complex to the centrosome, providing microtubule nucleation sites. The present study suggests the possible involvement of DISC1 in the pathophysiology of mental disorders due to its putative effect on centrosomal function.

KW - Affective disorders

KW - Centrosome

KW - DISC1

KW - Kendrin

KW - Mental disorders

KW - Pericentrin

KW - Schizophrenia

UR - http://www.scopus.com/inward/record.url?scp=1942504197&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=1942504197&partnerID=8YFLogxK

U2 - 10.1016/j.bbrc.2004.03.163

DO - 10.1016/j.bbrc.2004.03.163

M3 - Article

VL - 317

SP - 1195

EP - 1199

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

SN - 0006-291X

IS - 4

ER -