Directed disruption of the Chlamydomonas chloroplast psbK gene destabilizes the photosystem II reaction center complex

Yuichiro Takahashi, Hideki Matsumoto, Michel Goldschmidt-Clermont, Jean David Rochaix

Research output: Contribution to journalArticle

57 Citations (Scopus)

Abstract

Using particle gun-mediated chloroplast transformation we have disrupted the psbK gene of Chlamydomonas reihardtii with an aadA expression cassette that confers resistance to spectinomycin. The transformants are unable to grow photoautotrophically, but they grow normally in acetate-containing medium. They are deficient in photosystem II activity as measured by fluorescence transients and O2 evolution and they accumulate less than 10% of wild-type levels of photosystem II as measured by immunochemical means. Pulse-labeling experiments indicate that the photosystem II complex is synthesized normally in the transformants. These results differ from those obtained previously with similar cyanobacterial psbK mutants that were still capable of photoautotrophic growth (Ikeuchi et al., J. Biol. Chem. 266 (1991) 1111-1115). In C. reinhardtii the psbK product is required for the stable assembly and/or stability of the photosystem II complex and essential for photoautotrophic growth. The data also suggest that the stability requirements of the photosynthetic complexes differ considerably between C. reinhardtii and cyanobacteria.

Original languageEnglish
Pages (from-to)779-788
Number of pages10
JournalPlant Molecular Biology
Volume24
Issue number5
DOIs
Publication statusPublished - Mar 1994

Fingerprint

Chloroplast Genes
Chlamydomonas
Photosystem II Protein Complex
photosystem II
chloroplasts
Genes
genes
Photosynthetic Reaction Center Complex Proteins
Spectinomycin
spectinomycin
Firearms
Cyanobacteria
Chloroplasts
Growth
Labeling
Acetates
Fluorescence
acetates
fluorescence
mutants

Keywords

  • Chlamydomonas reinhardtii
  • chloroplast
  • photosystem II
  • psbK
  • transformation

ASJC Scopus subject areas

  • Biochemistry
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Directed disruption of the Chlamydomonas chloroplast psbK gene destabilizes the photosystem II reaction center complex. / Takahashi, Yuichiro; Matsumoto, Hideki; Goldschmidt-Clermont, Michel; Rochaix, Jean David.

In: Plant Molecular Biology, Vol. 24, No. 5, 03.1994, p. 779-788.

Research output: Contribution to journalArticle

Takahashi, Yuichiro ; Matsumoto, Hideki ; Goldschmidt-Clermont, Michel ; Rochaix, Jean David. / Directed disruption of the Chlamydomonas chloroplast psbK gene destabilizes the photosystem II reaction center complex. In: Plant Molecular Biology. 1994 ; Vol. 24, No. 5. pp. 779-788.
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