Direct targeting of proteins to lipid droplets demonstrated by time-lapse live cell imaging

Torahiko Tanaka, Kazumichi Kuroda, Masanori Ikeda, Nobuyuki Kato, Kazufumi Shimizu, Makoto Makishima

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

A protein that specifically targets lipid droplets (LDs) was created by connecting two domains of nonstructural protein 4B containing amphipathic helices from hepatitis C virus. We demonstrated its direct targeting and accumulation to the LD surface by time-lapse live cell imaging, comparable to those observed with adipose differentiation-related protein.

Original languageEnglish
Pages (from-to)620-623
Number of pages4
JournalJournal of Bioscience and Bioengineering
Volume116
Issue number5
DOIs
Publication statusPublished - Nov 2013

Fingerprint

Lipids
Proteins
Imaging techniques
Hepacivirus
Viruses
Lipid Droplets
Lipid Droplet Associated Proteins
Perilipin-2
Protein Domains

Keywords

  • Adipose differentiation-related protein
  • Hepatitis C virus
  • Lipid droplets
  • Nonstructural protein 4B
  • Time-lapse imaging

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology
  • Bioengineering

Cite this

Direct targeting of proteins to lipid droplets demonstrated by time-lapse live cell imaging. / Tanaka, Torahiko; Kuroda, Kazumichi; Ikeda, Masanori; Kato, Nobuyuki; Shimizu, Kazufumi; Makishima, Makoto.

In: Journal of Bioscience and Bioengineering, Vol. 116, No. 5, 11.2013, p. 620-623.

Research output: Contribution to journalArticle

Tanaka, Torahiko ; Kuroda, Kazumichi ; Ikeda, Masanori ; Kato, Nobuyuki ; Shimizu, Kazufumi ; Makishima, Makoto. / Direct targeting of proteins to lipid droplets demonstrated by time-lapse live cell imaging. In: Journal of Bioscience and Bioengineering. 2013 ; Vol. 116, No. 5. pp. 620-623.
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