Direct observation of the structural change of Tyr174 in the primary reaction of sensory Rhodopsin II

Misao Mizuno, Yuki Sudo, Michio Homma, Yasuhisa Mizutani

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Sensory rhodopsin II (SRII) is a negative phototaxis receptor containing retinal as its chromophore, which mediates the avoidance of blue light. The signal transduction is initiated by the photoisomerization of the retinal chromophore, resulting in conformational changes of the protein which are transmitted to a transducer protein. To gain insight into the SRII sensing mechanism, we employed time-resolved ultraviolet resonance Raman spectroscopy monitoring changes in the protein structure in the picosecond time range following photoisomerization. We used a 450 nm pump pulse to initiate the SRII photocycle and two kinds of probe pulses with wavelengths of 225 and 238 nm to detect spectral changes in the tryptophan and tyrosine bands, respectively. The observed spectral changes of the Raman bands are most likely due to tryptophan and tyrosine residues located in the vicinity of the retinal chromophore, i.e., Trp76, Trp171, Tyr51, or Tyr174. The 225 nm UVRR spectra exhibited bleaching of the intensity for all the tryptophan bands within the instrumental response time, followed by a partial recovery with a time constant of 30 ps and no further changes up to 1 ns. In the 238 nm UVRR spectra, a fast recovering component was observed in addition to the 30 ps time constant component. A comparison between the spectra of the WT and Y174F mutant of SRII indicates that Tyr174 changes its structure and/or environment upon chromophore photoisomerization. These data represent the first real-time observation of the structural change of Tyr174, of which functional importance was pointed out previously.

Original languageEnglish
Pages (from-to)3170-3180
Number of pages11
JournalBiochemistry
Volume50
Issue number15
DOIs
Publication statusPublished - Apr 19 2011
Externally publishedYes

Fingerprint

Sensory Rhodopsins
Chromophores
Photoisomerization
Observation
Tryptophan
Tyrosine
Signal transduction
Proteins
Bleaching
Raman Spectrum Analysis
Raman spectroscopy
Transducers
Reaction Time
Pumps
Signal Transduction
Recovery
Wavelength
Monitoring
Light

ASJC Scopus subject areas

  • Biochemistry

Cite this

Direct observation of the structural change of Tyr174 in the primary reaction of sensory Rhodopsin II. / Mizuno, Misao; Sudo, Yuki; Homma, Michio; Mizutani, Yasuhisa.

In: Biochemistry, Vol. 50, No. 15, 19.04.2011, p. 3170-3180.

Research output: Contribution to journalArticle

Mizuno, Misao ; Sudo, Yuki ; Homma, Michio ; Mizutani, Yasuhisa. / Direct observation of the structural change of Tyr174 in the primary reaction of sensory Rhodopsin II. In: Biochemistry. 2011 ; Vol. 50, No. 15. pp. 3170-3180.
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