Direct interactions of methamphetamine with the nucleus

Masato Asanuma, Teruo Hayashi, Sonia V. Ordonèz, Norio Ogawa, Jean Lud Cadet

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Possible direct effects of methamphetamine (METH) on transcription factors AP-1 and cAMP response element-binding protein (CREB) in the nucleus were assessed by electrophoretic mobility-shift assay. In vitro addition of METH to nuclear extract from brain tissue increased DNA-binding activities of both transcription factors. In addition, injections of METH to mice induced increases in the binding of AP-1 and CREB, which were depleted by preincubating the nuclear extract with anti-METH antibody. We also examined the cellular distribution of METH in mesencephalic neuronal cells using an immunofluorescence experiment with anti-METH antibody. METH-like immunoreactivity was seen to accumulate in the cytosol 4-6 h after the METH treatment. Furthermore, METH-positive signals were also observed in the nuclei of the METH-treated cells. The present study is the first demonstration that METH can have direct effects on DNA-binding protein complex by redistributing not only in the cytosol but also into the nucleus. (C) 2000 Elsevier Science B.V.

Original languageEnglish
Pages (from-to)237-243
Number of pages7
JournalMolecular Brain Research
Volume80
Issue number2
DOIs
Publication statusPublished - Sep 15 2000

Fingerprint

Methamphetamine
Cyclic AMP Response Element-Binding Protein
Transcription Factor AP-1
Cytosol
Anti-Idiotypic Antibodies
Tissue Extracts
DNA-Binding Proteins
Electrophoretic Mobility Shift Assay
Fluorescent Antibody Technique
Transcription Factors

Keywords

  • AP-1
  • Confocal laser scanning microscopy
  • CREB
  • Methamphetamine
  • Nucleus
  • Transcription factor

ASJC Scopus subject areas

  • Molecular Biology
  • Cellular and Molecular Neuroscience

Cite this

Direct interactions of methamphetamine with the nucleus. / Asanuma, Masato; Hayashi, Teruo; Ordonèz, Sonia V.; Ogawa, Norio; Cadet, Jean Lud.

In: Molecular Brain Research, Vol. 80, No. 2, 15.09.2000, p. 237-243.

Research output: Contribution to journalArticle

Asanuma, M, Hayashi, T, Ordonèz, SV, Ogawa, N & Cadet, JL 2000, 'Direct interactions of methamphetamine with the nucleus', Molecular Brain Research, vol. 80, no. 2, pp. 237-243. https://doi.org/10.1016/S0169-328X(00)00128-5
Asanuma, Masato ; Hayashi, Teruo ; Ordonèz, Sonia V. ; Ogawa, Norio ; Cadet, Jean Lud. / Direct interactions of methamphetamine with the nucleus. In: Molecular Brain Research. 2000 ; Vol. 80, No. 2. pp. 237-243.
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