Direct interaction analysis of microRNA-351-5p and nuclear scaffold lamin B1 mRNA by the cell-free in vitro mRNA/miRNA binding evaluation system

Akira Sato, Yoko Ogino, Akira Shimotsuma, Akiko Hiramoto, Hye Sook Kim, Yusuke Wataya

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)

Abstract

We previously demonstrated that miR-351-5p regulates nuclear scaffold lamin B1 expression and mediates the anticancer floxuridine-induced necrosis shift to apoptosis in mammalian tumor cells. Notably, it is unknown whether lamin B1 mRNA is a direct target of miR-351-5p. Here, we show that miR-351-5p interacts with a lamin B1 mRNA partial sequence by using the cell-free in vitro miRNA and mRNA binding evaluation system. In addition, the interaction of miR-351-5p/lamin B1 mRNA was suppressed by an miR-351-5p inhibitor. Our findings are important in exploring the functions of miRNAs in cellular processes, including cell death.

Original languageEnglish
Pages (from-to)799-805
Number of pages7
JournalNucleosides, Nucleotides and Nucleic Acids
Volume39
Issue number6
DOIs
Publication statusPublished - Jun 2 2020

Keywords

  • MicroRNA/mRNA interaction
  • lamin B1 mRNA
  • miRNA inhibitor
  • miRNA-351-5p

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Medicine
  • Genetics

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