Direct evidence of the glucose uptake into cochlear strial marginal cells: Application of a fluorescent tracer method combined with immunohistochemistry

Sohta Hishikawa, Midori Edamatsu, Risa Inoue-Ikeda, Motonori Ando

Research output: Contribution to journalArticle

Abstract

Despite the importance of glucose as an energy source for marginal cells of the cochlear stria vascularis, no direct evidence of the glucose uptake into these cells has been demonstrated. In this study, we investigated the glucose pathway in strial marginal cells using a fluorescent tracer method combined with immunohistochemistry. For glucose imaging, a non-metabolizable fluorescent glucose analog, 6-[N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino]-6-deoxyglucose (6-NBDG), was intravenously injected into mice. After 6-NBDG fluorescent signals in cochlear sections were observed, the same sections were processed for immunohistochemistry to identify marginal cells. This showed that 6-NBDG fluorescent signals had passed into the cytosol of marginal cells via Na+/K+-ATPase-positive basolateral membranes. These results provide direct physiological evidence of the glucose uptake into epithelial strial marginal cells. This combined method can help in examining the relationship between target molecules and functional proteins using the same tissue samples.
Original languageEnglish
Pages (from-to)1-8
Number of pages8
JournalBioimages
Volume23
Publication statusPublished - 2015

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Cochlea
Immunohistochemistry
Glucose
Stria Vascularis
Cytosol
Membranes
6-deoxy-N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)aminoglucose
Proteins

Cite this

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title = "Direct evidence of the glucose uptake into cochlear strial marginal cells: Application of a fluorescent tracer method combined with immunohistochemistry",
abstract = "Despite the importance of glucose as an energy source for marginal cells of the cochlear stria vascularis, no direct evidence of the glucose uptake into these cells has been demonstrated. In this study, we investigated the glucose pathway in strial marginal cells using a fluorescent tracer method combined with immunohistochemistry. For glucose imaging, a non-metabolizable fluorescent glucose analog, 6-[N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino]-6-deoxyglucose (6-NBDG), was intravenously injected into mice. After 6-NBDG fluorescent signals in cochlear sections were observed, the same sections were processed for immunohistochemistry to identify marginal cells. This showed that 6-NBDG fluorescent signals had passed into the cytosol of marginal cells via Na+/K+-ATPase-positive basolateral membranes. These results provide direct physiological evidence of the glucose uptake into epithelial strial marginal cells. This combined method can help in examining the relationship between target molecules and functional proteins using the same tissue samples.",
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T1 - Direct evidence of the glucose uptake into cochlear strial marginal cells: Application of a fluorescent tracer method combined with immunohistochemistry

AU - Hishikawa, Sohta

AU - Edamatsu, Midori

AU - Inoue-Ikeda, Risa

AU - Ando, Motonori

PY - 2015

Y1 - 2015

N2 - Despite the importance of glucose as an energy source for marginal cells of the cochlear stria vascularis, no direct evidence of the glucose uptake into these cells has been demonstrated. In this study, we investigated the glucose pathway in strial marginal cells using a fluorescent tracer method combined with immunohistochemistry. For glucose imaging, a non-metabolizable fluorescent glucose analog, 6-[N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino]-6-deoxyglucose (6-NBDG), was intravenously injected into mice. After 6-NBDG fluorescent signals in cochlear sections were observed, the same sections were processed for immunohistochemistry to identify marginal cells. This showed that 6-NBDG fluorescent signals had passed into the cytosol of marginal cells via Na+/K+-ATPase-positive basolateral membranes. These results provide direct physiological evidence of the glucose uptake into epithelial strial marginal cells. This combined method can help in examining the relationship between target molecules and functional proteins using the same tissue samples.

AB - Despite the importance of glucose as an energy source for marginal cells of the cochlear stria vascularis, no direct evidence of the glucose uptake into these cells has been demonstrated. In this study, we investigated the glucose pathway in strial marginal cells using a fluorescent tracer method combined with immunohistochemistry. For glucose imaging, a non-metabolizable fluorescent glucose analog, 6-[N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino]-6-deoxyglucose (6-NBDG), was intravenously injected into mice. After 6-NBDG fluorescent signals in cochlear sections were observed, the same sections were processed for immunohistochemistry to identify marginal cells. This showed that 6-NBDG fluorescent signals had passed into the cytosol of marginal cells via Na+/K+-ATPase-positive basolateral membranes. These results provide direct physiological evidence of the glucose uptake into epithelial strial marginal cells. This combined method can help in examining the relationship between target molecules and functional proteins using the same tissue samples.

M3 - Article

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