Direct evidence of cytosolic PNGase activity in Arabidopsis thaliana: In vitro assay system for plant cPNGase activity

Sahoko Shirai, Ryota Uemura, Megumi Maeda, Hiroyuki Kajiura, Ryo Misaki, Kazuhito Fujiyama, Yoshinobu Kimura

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

Cytosolic peptide:N-glycanase (cPNGase), which occurs ubiquitously in eukaryotic cells, is involved in the de-N-glycosylation of misfolded glycoproteins in the protein quality control system. In this study, we aimed to provide direct evidence of plant cPNGase activity against a denatured glycoprotein using a crude extract prepared from a mutant line of Arabidopsis thaliana lacking 2 acidic PNGase genes.

Original languageEnglish
Pages (from-to)1460-1463
Number of pages4
JournalBioscience, Biotechnology and Biochemistry
Volume85
Issue number6
DOIs
Publication statusPublished - Jun 1 2021

Keywords

  • Arabidopsis thaliana
  • PNGase assay
  • acidic PNGase
  • cytosolic PNGase
  • free N-glycan

ASJC Scopus subject areas

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

Fingerprint

Dive into the research topics of 'Direct evidence of cytosolic PNGase activity in Arabidopsis thaliana: In vitro assay system for plant cPNGase activity'. Together they form a unique fingerprint.

Cite this