Direct evidence for a crucial role of the arterial wall in control of atherosclerosis susceptibility

Hong Pei, Yinong Wang, Toru Miyoshi, Zhimin Zhang, Alan H. Matsumoto, Gregory A. Helm, George Tellides, Weibin Shi

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19 Citations (Scopus)

Abstract

BACKGROUND - Inbred mouse strains C57BL/6J (B6) and C3H/HeJ (C3H) exhibit marked differences in atherosclerosis susceptibility. We sought to determine whether the difference in atherosclerosis susceptibility resides at the level of arterial walls. METHODS AND RESULTS - Thoracic aortic segments from 8-week-old female B6 and C3H apolipoprotein E-deficient mice were transplanted into the infrarenal aorta of 10-week-old female F1 mice. After transplantation, recipients were maintained on a chow diet for 16 weeks. The donor aortic segments of B6 mice developed significantly larger atherosclerotic lesions than those of C3H (44 983±11 702 versus 5600±4885 μm per section; P=0.011). Expression of vascular cell adhesion molecule (VCAM)-1 by endothelial cells was examined both in vitro and in vivo. B6 mice expressed significantly more VCAM-1 than their C3H counterparts. Sequence analysis of VCAM-1 cDNA revealed a nucleotide difference in the coding region that resulted in substitution of an amino acid in the protein product. CONCLUSIONS - These data provide direct proof that factors operating in the vessel wall, particularly endothelial cells, can serve as atherosclerosis modifiers and suggest a possibility for the contribution of VCAM-1 to atherosclerosis susceptibility.

Original languageEnglish
Pages (from-to)2382-2389
Number of pages8
JournalCirculation
Volume114
Issue number22
DOIs
Publication statusPublished - Nov 1 2006

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Keywords

  • Atherosclerosis
  • Endothelium
  • Grafting
  • Transplantation

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine
  • Physiology (medical)

Cite this

Pei, H., Wang, Y., Miyoshi, T., Zhang, Z., Matsumoto, A. H., Helm, G. A., Tellides, G., & Shi, W. (2006). Direct evidence for a crucial role of the arterial wall in control of atherosclerosis susceptibility. Circulation, 114(22), 2382-2389. https://doi.org/10.1161/CIRCULATIONAHA.106.640185