Direct evidence for a crucial role of the arterial wall in control of atherosclerosis susceptibility

Hong Pei, Yinong Wang, Toru Miyoshi, Zhimin Zhang, Alan H. Matsumoto, Gregory A. Helm, George Tellides, Weibin Shi

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

BACKGROUND - Inbred mouse strains C57BL/6J (B6) and C3H/HeJ (C3H) exhibit marked differences in atherosclerosis susceptibility. We sought to determine whether the difference in atherosclerosis susceptibility resides at the level of arterial walls. METHODS AND RESULTS - Thoracic aortic segments from 8-week-old female B6 and C3H apolipoprotein E-deficient mice were transplanted into the infrarenal aorta of 10-week-old female F1 mice. After transplantation, recipients were maintained on a chow diet for 16 weeks. The donor aortic segments of B6 mice developed significantly larger atherosclerotic lesions than those of C3H (44 983±11 702 versus 5600±4885 μm per section; P=0.011). Expression of vascular cell adhesion molecule (VCAM)-1 by endothelial cells was examined both in vitro and in vivo. B6 mice expressed significantly more VCAM-1 than their C3H counterparts. Sequence analysis of VCAM-1 cDNA revealed a nucleotide difference in the coding region that resulted in substitution of an amino acid in the protein product. CONCLUSIONS - These data provide direct proof that factors operating in the vessel wall, particularly endothelial cells, can serve as atherosclerosis modifiers and suggest a possibility for the contribution of VCAM-1 to atherosclerosis susceptibility.

Original languageEnglish
Pages (from-to)2382-2389
Number of pages8
JournalCirculation
Volume114
Issue number22
DOIs
Publication statusPublished - Nov 2006
Externally publishedYes

Fingerprint

Vascular Cell Adhesion Molecule-1
Atherosclerosis
Endothelial Cells
Inbred Strains Mice
Apolipoproteins E
Amino Acid Substitution
Sequence Analysis
Aorta
Thorax
Nucleotides
Complementary DNA
Transplantation
Diet
Proteins

Keywords

  • Atherosclerosis
  • Endothelium
  • Grafting
  • Transplantation

ASJC Scopus subject areas

  • Physiology
  • Cardiology and Cardiovascular Medicine

Cite this

Direct evidence for a crucial role of the arterial wall in control of atherosclerosis susceptibility. / Pei, Hong; Wang, Yinong; Miyoshi, Toru; Zhang, Zhimin; Matsumoto, Alan H.; Helm, Gregory A.; Tellides, George; Shi, Weibin.

In: Circulation, Vol. 114, No. 22, 11.2006, p. 2382-2389.

Research output: Contribution to journalArticle

Pei, H, Wang, Y, Miyoshi, T, Zhang, Z, Matsumoto, AH, Helm, GA, Tellides, G & Shi, W 2006, 'Direct evidence for a crucial role of the arterial wall in control of atherosclerosis susceptibility', Circulation, vol. 114, no. 22, pp. 2382-2389. https://doi.org/10.1161/CIRCULATIONAHA.106.640185
Pei, Hong ; Wang, Yinong ; Miyoshi, Toru ; Zhang, Zhimin ; Matsumoto, Alan H. ; Helm, Gregory A. ; Tellides, George ; Shi, Weibin. / Direct evidence for a crucial role of the arterial wall in control of atherosclerosis susceptibility. In: Circulation. 2006 ; Vol. 114, No. 22. pp. 2382-2389.
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