Direct detection of Epstein-Barr virus DNA from a single Reed-Sternberg cell of Hodgkin's disease by polymerase chain reaction

Norihiro Teramoto, Tadaatsu Akagi, Tadashi Yoshino, Kiyoshi Takahashi, Ho Jong Jeon

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Eleven cases of Hodgkin's disease (HD) were examined for the presence of the Epstein-Barr virus (EBV) genome, using the polymerase chain reaction (PCR) to detect EBV DNA in whole paraffin-embedded tissue specimens and in single cells picked out from the specimens with a micromanipulator. The EBV genome was detected in 5 of the 11 cases by conventional PCR. Single cell PCR demonstrated the EBV genome in Reed-Sternberg cells from all the EBV-positive cases, but not from any of the EBV-negative cases. Background lymphocytes and lysozyme-positive histiocytes from EBV-positive cases did not contain the EBV genome. These results indicate an etiological association of EBV with some cases of HD.

Original languageEnglish
Pages (from-to)329-333
Number of pages5
JournalJapanese Journal of Cancer Research
Volume83
Issue number4
Publication statusPublished - Apr 1992

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Reed-Sternberg Cells
Human Herpesvirus 4
Hodgkin Disease
Polymerase Chain Reaction
DNA
Genome
Histiocytes
Muramidase
Paraffin
Lymphocytes

Keywords

  • Epstein-Barr virus
  • Hodgkin's disease
  • Micromanipulator
  • PCR

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Direct detection of Epstein-Barr virus DNA from a single Reed-Sternberg cell of Hodgkin's disease by polymerase chain reaction. / Teramoto, Norihiro; Akagi, Tadaatsu; Yoshino, Tadashi; Takahashi, Kiyoshi; Jeon, Ho Jong.

In: Japanese Journal of Cancer Research, Vol. 83, No. 4, 04.1992, p. 329-333.

Research output: Contribution to journalArticle

Teramoto, Norihiro ; Akagi, Tadaatsu ; Yoshino, Tadashi ; Takahashi, Kiyoshi ; Jeon, Ho Jong. / Direct detection of Epstein-Barr virus DNA from a single Reed-Sternberg cell of Hodgkin's disease by polymerase chain reaction. In: Japanese Journal of Cancer Research. 1992 ; Vol. 83, No. 4. pp. 329-333.
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