Dipeptidyl compounds ameliorate the serumdeprivation-induced reduction in cell viability via the neurotrophin-activating effect in SH-SY5Y cells

Ken ichi Tanaka, Hiroya Ogo, Hiroaki Kaji, Kaori Miyatake, Erika Tokudome, Kanako Sonoda, Norio Ogawa, Masato Asanuma

Research output: Contribution to journalArticle

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Abstract

Objectives and methods: We have searched for low-molecular-weight compounds as potent new nonimmunosuppressive immunophilin ligands (NI-IPLs) that are stronger than existent NI-IPLs such as GPI1046 and/or V10367 from the perspective of neuroprotective efficacy. We selected six dipeptidyl compounds as new NI-IPL candidates, and first examined the effects of each of these compounds on the serum-deprivation-induced reduction in the viability of SH-SY5Y cells. In addition, we clarified the effects of these compounds on neurotrophin release into medium in SH-SY5Y cells. Results: Pre-treatment with Leu-Ile and Ile-Ile prevented the serum deprivation-induced reduction in cell viability in SH-SY5Y cells. In naive SH-SY5Y cells, treatment with Leu-Ile and Ile-Ile for 24 hours significantly increased both brain-derived neurotrophic factor and glial cell-line-derived neurotrophic factor releases in comparison with relative vehicle treatments. Moreover, none of the dipeptidyl compounds could prevent the concanavalin A-induced enhancement in interleukin-2 and interleukin-4 release in mouse spleen cells. Discussion: The immunosuppressive effect is not essential to the neuroprotective properties of dipeptidyl compounds, and Leu-Ile and Ile-Ile have neurotrophin-activating effects, like FK506 and its existing non-immunosuppressive derivatives.

Original languageEnglish
Pages (from-to)619-622
Number of pages4
JournalNeurological Research
Volume34
Issue number6
DOIs
Publication statusPublished - Jul 2012

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Nerve Growth Factors
Immunophilins
Cell Survival
Ligands
Glial Cell Line-Derived Neurotrophic Factor
Brain-Derived Neurotrophic Factor
Tacrolimus
Immunosuppressive Agents
Concanavalin A
Serum
Interleukin-4
Interleukin-2
Spleen
Molecular Weight

Keywords

  • Dipeptide
  • Leu-ile
  • Mmunophilin ligand
  • Neuroprotection
  • Neurotrophin

ASJC Scopus subject areas

  • Clinical Neurology
  • Neurology

Cite this

Dipeptidyl compounds ameliorate the serumdeprivation-induced reduction in cell viability via the neurotrophin-activating effect in SH-SY5Y cells. / Tanaka, Ken ichi; Ogo, Hiroya; Kaji, Hiroaki; Miyatake, Kaori; Tokudome, Erika; Sonoda, Kanako; Ogawa, Norio; Asanuma, Masato.

In: Neurological Research, Vol. 34, No. 6, 07.2012, p. 619-622.

Research output: Contribution to journalArticle

Tanaka, Ken ichi ; Ogo, Hiroya ; Kaji, Hiroaki ; Miyatake, Kaori ; Tokudome, Erika ; Sonoda, Kanako ; Ogawa, Norio ; Asanuma, Masato. / Dipeptidyl compounds ameliorate the serumdeprivation-induced reduction in cell viability via the neurotrophin-activating effect in SH-SY5Y cells. In: Neurological Research. 2012 ; Vol. 34, No. 6. pp. 619-622.
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