Digestion of extracellular DNA is required for giant colony formation of Staphylococcus aureus

Chikara Kaito, Takanori Hirano, Yosuke Omae, Kazuhisa Sekimizu

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Staphylococcus aureus spreads on soft agar surfaces and forms giant colonies. Here, we examined the inhibitory role of extracellular DNA on the colony spreading activity. The double-deletion mutation of nuc1 and nuc2, which encode secretory nucleases, increased extracellular DNA and showed a decreased ability to form giant colonies. The addition of DNase I or micrococcal nuclease to the soft agar restored the ability of the nuc1-nuc2 double mutant to form giant colonies. In addition, the promoter activities of nuc1 and nuc2 in the wild-type strain were elevated in the peripheral region of the giant colony. These findings suggest that the digestion of extracellular DNA by secretory nucleases is required for the colony spreading activity of S. aureus.

Original languageEnglish
Pages (from-to)142-148
Number of pages7
JournalMicrobial Pathogenesis
Volume51
Issue number3
DOIs
Publication statusPublished - Sep 1 2011
Externally publishedYes

Fingerprint

Agar
Staphylococcus aureus
Digestion
Micrococcal Nuclease
Deoxyribonucleases
Sequence Deletion
Deoxyribonuclease I
DNA

Keywords

  • Colony spreading
  • Extracellular DNA
  • Nuclease
  • Staphylococcus aureus

ASJC Scopus subject areas

  • Microbiology
  • Infectious Diseases

Cite this

Digestion of extracellular DNA is required for giant colony formation of Staphylococcus aureus. / Kaito, Chikara; Hirano, Takanori; Omae, Yosuke; Sekimizu, Kazuhisa.

In: Microbial Pathogenesis, Vol. 51, No. 3, 01.09.2011, p. 142-148.

Research output: Contribution to journalArticle

Kaito, Chikara ; Hirano, Takanori ; Omae, Yosuke ; Sekimizu, Kazuhisa. / Digestion of extracellular DNA is required for giant colony formation of Staphylococcus aureus. In: Microbial Pathogenesis. 2011 ; Vol. 51, No. 3. pp. 142-148.
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