Differentiation of steroid-producing cells during ovarian differentiation in the protogynous malabar grouper, epinephelus malabaricus

Ryosuke Murata, Hirofumi Karimata, Yasuhisa Kobayashi, Ryo Horiguchi, Kazuo Kishimoto, Motofumi Kimura, Tohru Kobayashi, Kiyoshi Soyano, Masaru Nakamura

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13 Citations (Scopus)

Abstract

To understand the mechanism of sex differentiation in the protogynous Malabar grouper Epinephelus malabaricus, we performed an immunohistochemical investigation of the expression of three steroidogenic enzymes, cholesterol-side-chain-cleavage enzyme (CYP11a), aromatase (CYP19a1a), and cytochrome P45011beta-hydroxylase (CYP11b), in the gonads during ovarian differentiation. Strong positive immunoreactivity against CYP11a, the key enzyme of steroidogenesis, and CYP19a1a which is essential for estrogen (17beta-estradiol) production, appeared first in the somatic cells surrounding gonial germ cells in undifferentiated gonads and throughout ovarian differentiation. However, positive immunoreactivity against CYP11b, which is important for androgen (11-ketotestosterone) production, first appeared in the cluster of somatic cells in the ovary tunica near the dorsal blood vessel after differentiation. CYP19a1a and CYP11b did not co-localize in any cells. These results indicate that there are two types of steroid-producing cells, estrogen-producing cells and androgen-producing cells, in the gonads of this fish, and they are distributed differently, suggesting that these cells are derived from different somatic cells. Estrogen-producing cells appeared prior to ovarian differentiation, while androgen-producing cells were first detected after ovarian differentiation. These results suggest that endogenous estrogen is involved in ovarian differentiation.

Original languageEnglish
Pages (from-to)619-625
Number of pages7
JournalInternational Journal of Developmental Biology
Volume55
Issue number6
DOIs
Publication statusPublished - 2011

Keywords

  • 11-ketotestosterone
  • 17beta-estradiol
  • Grouper
  • Protogynous hermaphrodite
  • Steroid-producing cell

ASJC Scopus subject areas

  • Embryology
  • Developmental Biology

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  • Cite this

    Murata, R., Karimata, H., Kobayashi, Y., Horiguchi, R., Kishimoto, K., Kimura, M., Kobayashi, T., Soyano, K., & Nakamura, M. (2011). Differentiation of steroid-producing cells during ovarian differentiation in the protogynous malabar grouper, epinephelus malabaricus. International Journal of Developmental Biology, 55(6), 619-625. https://doi.org/10.1387/ijdb.103181rm