Differentiation of murine B cells induced by chondroitin sulfate B

Ritsuko Yoshihara, Eriko Aoyama, Yusuke Kadota, Saeko Kawai, Tomomi Goto, Ming Zhong, Eiichi Gohda

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

A two-step culture system was used to investigate the role of chondroitin sulfate (CS) B, which is mitogenic to B cells, in differentiation of B cells. Mouse spleen B cells were incubated for 3 days with CSB in the presence of interleukin (IL)-4 and IL-5. After washing, the cells were replated at 105 viable cells/well and recultured without CSB in the presence of IL-4 and IL-5. CSB dose-dependently increased IgM production, the greatest enhancement being 450%. Dextran sulfate had a similar effect, whereas other glycosaminoglycans, CSA, CSC, heparin and hyaluronic acid, were marginally effective. Treatment of B cells with CSB resulted in increases in the number of IgM-secreting cells and numbers of CD138-positive cells and CD45R/B220-negative cells. CSB-induced IgM production was inhibited by the protein kinase C (PKC) inhibitor GF109203X but not by the phosphatidylinositol 3-kinase (PI3K) inhibitor wortmannin. These results demonstrated that CSB promoted differentiation of B cells in the presence of IL-4 and IL-5 and suggested that PKC but not PI3K is crucial for CSB-induced IgM production.

Original languageEnglish
Pages (from-to)14-23
Number of pages10
JournalCellular Immunology
Volume250
Issue number1-2
DOIs
Publication statusPublished - Nov 2007

Fingerprint

Dermatan Sulfate
B-Lymphocytes
Immunoglobulin M
Interleukin-5
Phosphatidylinositol 3-Kinase
Interleukin-4
Protein Kinase C
Dextran Sulfate
Protein C Inhibitor
Hyaluronic Acid
Protein Kinase Inhibitors
Glycosaminoglycans
Heparin
Cell Differentiation
Spleen
Cell Count

Keywords

  • CD138
  • Chondroitin sulfate B (CSB)
  • Differentiation
  • IgM
  • Murine B cells
  • Protein kinase C

ASJC Scopus subject areas

  • Cell Biology
  • Immunology

Cite this

Yoshihara, R., Aoyama, E., Kadota, Y., Kawai, S., Goto, T., Zhong, M., & Gohda, E. (2007). Differentiation of murine B cells induced by chondroitin sulfate B. Cellular Immunology, 250(1-2), 14-23. https://doi.org/10.1016/j.cellimm.2007.12.002

Differentiation of murine B cells induced by chondroitin sulfate B. / Yoshihara, Ritsuko; Aoyama, Eriko; Kadota, Yusuke; Kawai, Saeko; Goto, Tomomi; Zhong, Ming; Gohda, Eiichi.

In: Cellular Immunology, Vol. 250, No. 1-2, 11.2007, p. 14-23.

Research output: Contribution to journalArticle

Yoshihara, R, Aoyama, E, Kadota, Y, Kawai, S, Goto, T, Zhong, M & Gohda, E 2007, 'Differentiation of murine B cells induced by chondroitin sulfate B', Cellular Immunology, vol. 250, no. 1-2, pp. 14-23. https://doi.org/10.1016/j.cellimm.2007.12.002
Yoshihara, Ritsuko ; Aoyama, Eriko ; Kadota, Yusuke ; Kawai, Saeko ; Goto, Tomomi ; Zhong, Ming ; Gohda, Eiichi. / Differentiation of murine B cells induced by chondroitin sulfate B. In: Cellular Immunology. 2007 ; Vol. 250, No. 1-2. pp. 14-23.
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