Differentiation of monoblastic cell line UG3 into leukemic dendritic cells.

Nobuharu Fujii, Takashi Ikeda, Kazuma Ikeda, Akio Hiraki, Kimihiko Kawakami, Kozo Masuda, Yoshinobu Maeda, Kiyohiko Hatake, Kazuo Motoyoshi, Mine Harada, Mitsune Tanimoto

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Abstract

Dendritic cells (DCs) are known to be generated from leukemic clone from patients with acute and chronic leukemia when cultured in the presence of combination of granulocyte-macrophage colony-stimulating factor (GM-CSF), tumor necrosis factor-alpha (TNF-alpha) and interleukin-4. However, there have been few reports that showed DCs could be effectively differentiated from human leukemia cell lines. In this study, we have shown that a human monoblastic cell line, UG3, was inducible to differentiate into DCs in the presence of GM-CSF and TNF-alpha along monocyte-macrophage lineage. These DCs, consistently displayed dendritic morphology, phenotypes and allogeneic T-cell stimulating capacity. UG3 cells thus may represent a suitable model to further elucidate characteristics of DC differentiation.

Original languageEnglish
Pages (from-to)617-620
Number of pages4
JournalInternational journal of oncology
Volume21
Issue number3
Publication statusPublished - Sep 2002

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ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Fujii, N., Ikeda, T., Ikeda, K., Hiraki, A., Kawakami, K., Masuda, K., Maeda, Y., Hatake, K., Motoyoshi, K., Harada, M., & Tanimoto, M. (2002). Differentiation of monoblastic cell line UG3 into leukemic dendritic cells. International journal of oncology, 21(3), 617-620.