Differentiation of monoblastic cell line UG3 into leukemic dendritic cells.

Nobuharu Fujii; Takashi Ikeda; Kazuma Ikeda; Akio Hiraki; Kimihiko Kawakami; Kozo Masuda; Yoshinobu Maeda; Kiyohiko Hatake; Kazuo Motoyoshi; Mine Harada; Mitsune Tanimoto

doi:10.3892/ijo.21.3.617

Differentiation of monoblastic cell line UG3 into leukemic dendritic cells.

Nobuharu Fujii, Takashi Ikeda, Kazuma Ikeda, Akio Hiraki, Kimihiko Kawakami, Kozo Masuda, Yoshinobu Maeda, Kiyohiko Hatake, Kazuo Motoyoshi, Mine Harada, Mitsune Tanimoto

Research output: Contribution to journal › Article › peer-review

1 Citation (Scopus)

Abstract

Dendritic cells (DCs) are known to be generated from leukemic clone from patients with acute and chronic leukemia when cultured in the presence of combination of granulocyte-macrophage colony-stimulating factor (GM-CSF), tumor necrosis factor-alpha (TNF-alpha) and interleukin-4. However, there have been few reports that showed DCs could be effectively differentiated from human leukemia cell lines. In this study, we have shown that a human monoblastic cell line, UG3, was inducible to differentiate into DCs in the presence of GM-CSF and TNF-alpha along monocyte-macrophage lineage. These DCs, consistently displayed dendritic morphology, phenotypes and allogeneic T-cell stimulating capacity. UG3 cells thus may represent a suitable model to further elucidate characteristics of DC differentiation.

Original language	English
Pages (from-to)	617-620
Number of pages	4
Journal	International journal of oncology
Volume	21
Issue number	3
DOIs	https://doi.org/10.3892/ijo.21.3.617
Publication status	Published - Sep 2002

ASJC Scopus subject areas

Oncology
Cancer Research

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Differentiation of monoblastic cell line UG3 into leukemic dendritic cells. / Fujii, Nobuharu; Ikeda, Takashi; Ikeda, Kazuma; Hiraki, Akio; Kawakami, Kimihiko; Masuda, Kozo; Maeda, Yoshinobu; Hatake, Kiyohiko; Motoyoshi, Kazuo; Harada, Mine; Tanimoto, Mitsune.

In: International journal of oncology, Vol. 21, No. 3, 09.2002, p. 617-620.

Research output: Contribution to journal › Article › peer-review

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title = "Differentiation of monoblastic cell line UG3 into leukemic dendritic cells.",

abstract = "Dendritic cells (DCs) are known to be generated from leukemic clone from patients with acute and chronic leukemia when cultured in the presence of combination of granulocyte-macrophage colony-stimulating factor (GM-CSF), tumor necrosis factor-alpha (TNF-alpha) and interleukin-4. However, there have been few reports that showed DCs could be effectively differentiated from human leukemia cell lines. In this study, we have shown that a human monoblastic cell line, UG3, was inducible to differentiate into DCs in the presence of GM-CSF and TNF-alpha along monocyte-macrophage lineage. These DCs, consistently displayed dendritic morphology, phenotypes and allogeneic T-cell stimulating capacity. UG3 cells thus may represent a suitable model to further elucidate characteristics of DC differentiation.",

author = "Nobuharu Fujii and Takashi Ikeda and Kazuma Ikeda and Akio Hiraki and Kimihiko Kawakami and Kozo Masuda and Yoshinobu Maeda and Kiyohiko Hatake and Kazuo Motoyoshi and Mine Harada and Mitsune Tanimoto",

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AU - Fujii, Nobuharu

AU - Ikeda, Takashi

AU - Ikeda, Kazuma

AU - Hiraki, Akio

AU - Kawakami, Kimihiko

AU - Masuda, Kozo

AU - Maeda, Yoshinobu

AU - Hatake, Kiyohiko

AU - Motoyoshi, Kazuo

AU - Harada, Mine

AU - Tanimoto, Mitsune

PY - 2002/9

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AB - Dendritic cells (DCs) are known to be generated from leukemic clone from patients with acute and chronic leukemia when cultured in the presence of combination of granulocyte-macrophage colony-stimulating factor (GM-CSF), tumor necrosis factor-alpha (TNF-alpha) and interleukin-4. However, there have been few reports that showed DCs could be effectively differentiated from human leukemia cell lines. In this study, we have shown that a human monoblastic cell line, UG3, was inducible to differentiate into DCs in the presence of GM-CSF and TNF-alpha along monocyte-macrophage lineage. These DCs, consistently displayed dendritic morphology, phenotypes and allogeneic T-cell stimulating capacity. UG3 cells thus may represent a suitable model to further elucidate characteristics of DC differentiation.

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