TY - JOUR
T1 - Differentiation of monoblastic cell line UG3 into leukemic dendritic cells.
AU - Fujii, Nobuharu
AU - Ikeda, Takashi
AU - Ikeda, Kazuma
AU - Hiraki, Akio
AU - Kawakami, Kimihiko
AU - Masuda, Kozo
AU - Maeda, Yoshinobu
AU - Hatake, Kiyohiko
AU - Motoyoshi, Kazuo
AU - Harada, Mine
AU - Tanimoto, Mitsune
PY - 2002/9
Y1 - 2002/9
N2 - Dendritic cells (DCs) are known to be generated from leukemic clone from patients with acute and chronic leukemia when cultured in the presence of combination of granulocyte-macrophage colony-stimulating factor (GM-CSF), tumor necrosis factor-alpha (TNF-alpha) and interleukin-4. However, there have been few reports that showed DCs could be effectively differentiated from human leukemia cell lines. In this study, we have shown that a human monoblastic cell line, UG3, was inducible to differentiate into DCs in the presence of GM-CSF and TNF-alpha along monocyte-macrophage lineage. These DCs, consistently displayed dendritic morphology, phenotypes and allogeneic T-cell stimulating capacity. UG3 cells thus may represent a suitable model to further elucidate characteristics of DC differentiation.
AB - Dendritic cells (DCs) are known to be generated from leukemic clone from patients with acute and chronic leukemia when cultured in the presence of combination of granulocyte-macrophage colony-stimulating factor (GM-CSF), tumor necrosis factor-alpha (TNF-alpha) and interleukin-4. However, there have been few reports that showed DCs could be effectively differentiated from human leukemia cell lines. In this study, we have shown that a human monoblastic cell line, UG3, was inducible to differentiate into DCs in the presence of GM-CSF and TNF-alpha along monocyte-macrophage lineage. These DCs, consistently displayed dendritic morphology, phenotypes and allogeneic T-cell stimulating capacity. UG3 cells thus may represent a suitable model to further elucidate characteristics of DC differentiation.
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M3 - Article
C2 - 12168108
AN - SCOPUS:0036725911
VL - 21
SP - 617
EP - 620
JO - International Journal of Oncology
JF - International Journal of Oncology
SN - 1019-6439
IS - 3
ER -