Differential transcription of the MPB7O genes in two major groups of Mycobacterium bovis BCG substrains

T. Matsuo, S. Matsumoto, Naoya Oohara, H. Kitaura, A. Mizuno, T. Yamada

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Substrains of Mycobacterium bovis BCG (BCG) have been divided into two major groups, high and low producers, on the basis of the amount of secretion of the MPB7O protein. The antigen is produced in high concentration by BCG Tokyo, Moreau, Russia and Sweden (high-producer substrains), whereas in BCG Pasteur, Copenhagen and Tice (low-producer substrains) it is detected at 1% (w/w) or less of the concentration of BCG Tokyo. To investigate why this protein is secreted differently, the MPB7O genes of BCG Tokyo and Pasteur were cloned, sequenced and compared. The MPB7O genes in two substrains showed exactly the same sequence. Even the upstream and downstream regions of the MPB7O gene were identical. MPB7O gene expression was assessed by means of Northern hybridization analysis and reverse transcriptase polymerase chain reaction. The mRNA was clearly detected in BCG Tokyo, but at a very low level in BCG Pasteur. On the basis of these results, the difference in the secretion of the MPB7O protein between BCG Tokyo and Pasteur was attributed to differential transcription efficiencies.

Original languageEnglish
Pages (from-to)1601-1607
Number of pages7
JournalMicrobiology
Volume141
Issue number7
Publication statusPublished - 1995
Externally publishedYes

Fingerprint

Mycobacterium bovis
Tokyo
Genes
Proteins
Russia
Reverse Transcriptase Polymerase Chain Reaction
Sweden
Gene Expression
Antigens
Messenger RNA

Keywords

  • BCG substrains
  • MPB70
  • RT-PCR
  • Transcription

ASJC Scopus subject areas

  • Microbiology

Cite this

Matsuo, T., Matsumoto, S., Oohara, N., Kitaura, H., Mizuno, A., & Yamada, T. (1995). Differential transcription of the MPB7O genes in two major groups of Mycobacterium bovis BCG substrains. Microbiology, 141(7), 1601-1607.

Differential transcription of the MPB7O genes in two major groups of Mycobacterium bovis BCG substrains. / Matsuo, T.; Matsumoto, S.; Oohara, Naoya; Kitaura, H.; Mizuno, A.; Yamada, T.

In: Microbiology, Vol. 141, No. 7, 1995, p. 1601-1607.

Research output: Contribution to journalArticle

Matsuo, T, Matsumoto, S, Oohara, N, Kitaura, H, Mizuno, A & Yamada, T 1995, 'Differential transcription of the MPB7O genes in two major groups of Mycobacterium bovis BCG substrains', Microbiology, vol. 141, no. 7, pp. 1601-1607.
Matsuo, T. ; Matsumoto, S. ; Oohara, Naoya ; Kitaura, H. ; Mizuno, A. ; Yamada, T. / Differential transcription of the MPB7O genes in two major groups of Mycobacterium bovis BCG substrains. In: Microbiology. 1995 ; Vol. 141, No. 7. pp. 1601-1607.
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