Differential requirement of EGFR signaling for the expression of defective proventriculus gene in the Drosophila endoderm and ectoderm

Tetsuya Shirai, Atsushi Maehara, Naruto Kiritooshi, Fumio Matsuzaki, Hiroshi Handa, Hideki Nakagoshi

Research output: Contribution to journalArticlepeer-review

8 Citations (Scopus)

Abstract

A homeobox gene, defective proventriculus (dve), is expressed in various tissues including the ventral ectoderm and midgut. Here, we show the expression pattern of dve in the ventral ectoderm, in which dve expression is induced by Spitz, a ligand for Drosophila epidermal growth factor receptor (EGFR). In spitz mutants, dve expression is only lost in the ventral ectoderm and overexpression of Spitz induces ectopic dve activation in the ventral ectoderm. Dve expression in the middle midgut depends on Decapentaplegic (Dpp) signaling, while expression of a dominant-negative form of Drosophila EGFR (DER DN) also causes a marked decrease in dve expression in the middle midgut. Furthermore, heterozygous mutation of thick veins (tkv), a Dpp receptor, strongly enhances the effect of DERDN. These results indicate that EGFR signaling is crucial for dve expression in the ventral ectoderm and is required in the middle midgut where it cooperates with Dpp signaling.

Original languageEnglish
Pages (from-to)473-477
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume311
Issue number2
DOIs
Publication statusPublished - Nov 14 2003

Keywords

  • Defective proventriculus
  • Dpp
  • Drosophila
  • EGFR
  • Ectoderm
  • Endoderm
  • Gut
  • Homeobox
  • Spitz
  • Vein

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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