Differential regulation of MBP kinases by a glycoproptein elicitor and a polypeptide suppressor from Mycosphaerella pinodes in pea

Srinivasa Rao Uppalapati, Kazuhiro Toyoda, Ishiga Yasuhiro, Yuki Ichinose, Tomonori Shiraishi

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

A polypeptide fungal suppressor from a pea pathogen Mycosphaerella pinodes plays a key role in pathogenesis by suppressing elicitor-induced defense response(s) in pea (Pisum sativum L). In this study, we show that treatment of pea tissues with the polysaccharide elicitor secreted by M. pinodes results in rapid increased activation of two myelin basic protein (MBP)-dependent kinases p44 (≈44 kDa) and p48 (≈48 kDa) within 15-30 min upon elicitation. Interestingly, the suppressor inhibited the elicitor-induced activation of only p44 kinase. While the defense-inducing signalling molecules, chitosan and salicylic acid (SA) activated the p44 and p48 kinases, methyl jasmonate (MeJA) did not. The abiotic stress signals, abscisic acid (ABA), NaCl and wounding activated the p48 kinase alone. These results demonstrate that MAPKs are differentially activated in response to pathogen invasion and abiotic stress in pea. Furthermore, specific inhibition of elicitor-induced p44 kinase activation by a MAPKK inhibitor, PD098059 and protein kinase inhibitor, K252a correlated with the suppression of elicitor-induced phenylalanine ammonia lyase (PAL) gene expression, supporting a role for p44 in the elicitor-induced defense response(s) in pea. Inhibition of p44 by the phosphoinositide (PI) turnover inhibitor, neomycin (a fungal suppressor mimic), and potentiation of p44 by the diacylglycerol (DAG) kinase inhibitor, R59022 indicated that p44 may be acting downstream of (PI) metabolism. Taken together, our results indicate that suppressor of defense elicitation from M. pinodes acts through inhibition of a MAPK (p44), possibly through a PI signaling pathway, facilitating the establishment of basic compatibility during infection of pea.

Original languageEnglish
Pages (from-to)17-25
Number of pages9
JournalPhysiological and Molecular Plant Pathology
Volume64
Issue number1
DOIs
Publication statusPublished - Jan 2004

Fingerprint

Mycosphaerella pinodes
Myelin Basic Protein
Peas
protein kinases
Protein Kinases
polypeptides
peas
phosphotransferases (kinases)
Peptides
Phosphotransferases
Phosphatidylinositols
abiotic stress
R 59022
diacylglycerol kinase
mitogen-activated protein kinase kinase
Diacylglycerol Kinase
Phenylalanine Ammonia-Lyase
neomycin
pathogens
methyl jasmonate

Keywords

  • Basic-compatibility
  • Elicitor
  • MAPK
  • MBP kinase
  • Mycosphaerella pinodes
  • Pisum sativum
  • Suppressor

ASJC Scopus subject areas

  • Plant Science

Cite this

Differential regulation of MBP kinases by a glycoproptein elicitor and a polypeptide suppressor from Mycosphaerella pinodes in pea. / Rao Uppalapati, Srinivasa; Toyoda, Kazuhiro; Yasuhiro, Ishiga; Ichinose, Yuki; Shiraishi, Tomonori.

In: Physiological and Molecular Plant Pathology, Vol. 64, No. 1, 01.2004, p. 17-25.

Research output: Contribution to journalArticle

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