Differential molecular and cellular immune mechanisms of postoperative and LPS-induced ileus in mice and rats

Joachim Schmidt, Burkhard Stoffels, R. Savanh Chanthaphavong, Bettina M. Buchholz, Atsunori Nakao, Anthony J. Bauer

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Ileus is caused by the initiation of a complex cascade of molecular and cellular inflammatory responses within the intestinal muscularis, which might be species specific. Our objective was to investigate a possible immunological divergence in the mechanisms of postoperative- and endotoxin-induced ileus in C57BL/6 mice and Sprague-Dawley rats. Gastrointestinal transit (GIT) was measured at 24. h after the injurious stimulus. MPO-staining and F4/80 immunohistochemistry were used to quantify polymorphonuclear and monocyte infiltration of jejunal muscularis whole-mounts, and intestinal muscularis MCP-1, ICAM-1 and iNOS gene expression was assessed by RT-PCR. Intestinal muscularis subjected to in vivo surgical manipulation (SM) or LPS treatment was cultured for 24. h, and the liberation of nitric oxide and chemokines/cytokines into the culture medium was analyzed by Griess reaction and Luminex multiplex assay. Intestinal SM and lipopolysaccharide (LPS) (15. mg/kg) caused a significant delay in gastrointestinal transit, which was more severe in mice compared to rats in both injury models. Both SM- and LPS-triggered neutrophil and monocytic extravasation into the rat jejunal muscularis exceeded the cellular infiltration seen in mice. These results correlated with significantly greater increases in rat muscularis MCP-1 (syn. CCL2), ICAM-1 and iNOS message with more subsequent NO production after SM or LPS compared to mouse. The cultured muscularis obtained from SM mice released significantly more inflammatory proteins such as TNF-α, IL-1-α, IL-4 and GM-CSF compared to the manipulated rat muscularis. In contrast, LPS initiated the secretion of significantly more IL-1β by the inflamed rat muscularis compared to the mouse, but GM-CSF (syn. CSF2) liberation from mouse muscularis was markedly higher compared to LPS-treated rat muscularis. The data indicate that mechanistically the development of ileus in rat is mediated predominately through a leukocytic pathway consisting of chemotaxis, cellular extravasation and NO liberation. Whereas, the more intense mouse ileus evolves via a potent but injury-specific local cytokine response.

Original languageEnglish
Pages (from-to)49-58
Number of pages10
JournalCytokine
Volume59
Issue number1
DOIs
Publication statusPublished - Jul 2012
Externally publishedYes

Fingerprint

Ileus
Lipopolysaccharides
Rats
Gastrointestinal Transit
Intercellular Adhesion Molecule-1
Granulocyte-Macrophage Colony-Stimulating Factor
Interleukin-1
Infiltration
Cytokines
Wounds and Injuries
Chemotaxis
Inbred C57BL Mouse
Chemokines
Endotoxins
Interleukin-4
Sprague Dawley Rats
Culture Media
Monocytes
Gene expression
Nitric Oxide

Keywords

  • Endotoxin
  • Inflammation
  • Postoperative ileus
  • Sepsis
  • Surgery

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy
  • Hematology
  • Biochemistry
  • Molecular Biology

Cite this

Differential molecular and cellular immune mechanisms of postoperative and LPS-induced ileus in mice and rats. / Schmidt, Joachim; Stoffels, Burkhard; Savanh Chanthaphavong, R.; Buchholz, Bettina M.; Nakao, Atsunori; Bauer, Anthony J.

In: Cytokine, Vol. 59, No. 1, 07.2012, p. 49-58.

Research output: Contribution to journalArticle

Schmidt, J, Stoffels, B, Savanh Chanthaphavong, R, Buchholz, BM, Nakao, A & Bauer, AJ 2012, 'Differential molecular and cellular immune mechanisms of postoperative and LPS-induced ileus in mice and rats', Cytokine, vol. 59, no. 1, pp. 49-58. https://doi.org/10.1016/j.cyto.2012.03.012
Schmidt, Joachim ; Stoffels, Burkhard ; Savanh Chanthaphavong, R. ; Buchholz, Bettina M. ; Nakao, Atsunori ; Bauer, Anthony J. / Differential molecular and cellular immune mechanisms of postoperative and LPS-induced ileus in mice and rats. In: Cytokine. 2012 ; Vol. 59, No. 1. pp. 49-58.
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