Differential localizations of the myo-inositol transporters HMIT and SMIT1 in the cochlear stria vascularis

Research output: Contribution to journalArticle

Abstract

The cochlear stria vascularis produces endolymph and thereby plays an active role in inner ear homeostasis. We recently reported that the H+/myo-inositol cotransporter (HMIT) gene is expressed in the stria vascularis. Here, we examined the protein localization of HMIT and Na+/myo-inositol cotransporter 1 (SMIT1) in the stria vascularis by immunohistochemistry. HMIT and SMIT1 were detected in the lateral wall of the cochlear duct. HMIT was widely detected throughout the stria vascularis, while SMIT1 was enriched in the strial basal cells. To examine the localization of HMIT in the stria vascularis in more detail, dissociated strial cells were immunostained, which resulted in the detection of HMIT immunoreactivity in marginal cells. These results indicate that HMIT is expressed in marginal cells and basal cells of the stria vascularis, while SMIT1 expression is enriched in basal cells. We speculate that HMIT and SMIT1 may play important roles in the homeostasis of cochlear fluids, for example by participating in pH regulation and osmoregulation.

Original languageEnglish
Pages (from-to)88-93
Number of pages6
JournalNeuroscience Letters
Volume674
DOIs
Publication statusPublished - May 1 2018

Fingerprint

Stria Vascularis
Cochlea
Inositol
Homeostasis
Cochlear Duct
Endolymph
Osmoregulation
Inner Ear
Immunohistochemistry

Keywords

  • Endolymph
  • Inner ear
  • Osmoregulation
  • pH
  • Rat (Brown Norway)

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Differential localizations of the myo-inositol transporters HMIT and SMIT1 in the cochlear stria vascularis. / Edamatsu, Midori; Kondo, Yasuhiro; Ando, Motonori.

In: Neuroscience Letters, Vol. 674, 01.05.2018, p. 88-93.

Research output: Contribution to journalArticle

@article{2a8d924a97d74a93bfedee82788a7ff5,
title = "Differential localizations of the myo-inositol transporters HMIT and SMIT1 in the cochlear stria vascularis",
abstract = "The cochlear stria vascularis produces endolymph and thereby plays an active role in inner ear homeostasis. We recently reported that the H+/myo-inositol cotransporter (HMIT) gene is expressed in the stria vascularis. Here, we examined the protein localization of HMIT and Na+/myo-inositol cotransporter 1 (SMIT1) in the stria vascularis by immunohistochemistry. HMIT and SMIT1 were detected in the lateral wall of the cochlear duct. HMIT was widely detected throughout the stria vascularis, while SMIT1 was enriched in the strial basal cells. To examine the localization of HMIT in the stria vascularis in more detail, dissociated strial cells were immunostained, which resulted in the detection of HMIT immunoreactivity in marginal cells. These results indicate that HMIT is expressed in marginal cells and basal cells of the stria vascularis, while SMIT1 expression is enriched in basal cells. We speculate that HMIT and SMIT1 may play important roles in the homeostasis of cochlear fluids, for example by participating in pH regulation and osmoregulation.",
keywords = "Endolymph, Inner ear, Osmoregulation, pH, Rat (Brown Norway)",
author = "Midori Edamatsu and Yasuhiro Kondo and Motonori Ando",
year = "2018",
month = "5",
day = "1",
doi = "10.1016/j.neulet.2018.03.028",
language = "English",
volume = "674",
pages = "88--93",
journal = "Neuroscience Letters",
issn = "0304-3940",
publisher = "Elsevier Ireland Ltd",

}

TY - JOUR

T1 - Differential localizations of the myo-inositol transporters HMIT and SMIT1 in the cochlear stria vascularis

AU - Edamatsu, Midori

AU - Kondo, Yasuhiro

AU - Ando, Motonori

PY - 2018/5/1

Y1 - 2018/5/1

N2 - The cochlear stria vascularis produces endolymph and thereby plays an active role in inner ear homeostasis. We recently reported that the H+/myo-inositol cotransporter (HMIT) gene is expressed in the stria vascularis. Here, we examined the protein localization of HMIT and Na+/myo-inositol cotransporter 1 (SMIT1) in the stria vascularis by immunohistochemistry. HMIT and SMIT1 were detected in the lateral wall of the cochlear duct. HMIT was widely detected throughout the stria vascularis, while SMIT1 was enriched in the strial basal cells. To examine the localization of HMIT in the stria vascularis in more detail, dissociated strial cells were immunostained, which resulted in the detection of HMIT immunoreactivity in marginal cells. These results indicate that HMIT is expressed in marginal cells and basal cells of the stria vascularis, while SMIT1 expression is enriched in basal cells. We speculate that HMIT and SMIT1 may play important roles in the homeostasis of cochlear fluids, for example by participating in pH regulation and osmoregulation.

AB - The cochlear stria vascularis produces endolymph and thereby plays an active role in inner ear homeostasis. We recently reported that the H+/myo-inositol cotransporter (HMIT) gene is expressed in the stria vascularis. Here, we examined the protein localization of HMIT and Na+/myo-inositol cotransporter 1 (SMIT1) in the stria vascularis by immunohistochemistry. HMIT and SMIT1 were detected in the lateral wall of the cochlear duct. HMIT was widely detected throughout the stria vascularis, while SMIT1 was enriched in the strial basal cells. To examine the localization of HMIT in the stria vascularis in more detail, dissociated strial cells were immunostained, which resulted in the detection of HMIT immunoreactivity in marginal cells. These results indicate that HMIT is expressed in marginal cells and basal cells of the stria vascularis, while SMIT1 expression is enriched in basal cells. We speculate that HMIT and SMIT1 may play important roles in the homeostasis of cochlear fluids, for example by participating in pH regulation and osmoregulation.

KW - Endolymph

KW - Inner ear

KW - Osmoregulation

KW - pH

KW - Rat (Brown Norway)

UR - http://www.scopus.com/inward/record.url?scp=85044103616&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85044103616&partnerID=8YFLogxK

U2 - 10.1016/j.neulet.2018.03.028

DO - 10.1016/j.neulet.2018.03.028

M3 - Article

VL - 674

SP - 88

EP - 93

JO - Neuroscience Letters

JF - Neuroscience Letters

SN - 0304-3940

ER -