Abstract
In a previous paper, we demonstrated that cultured human fibroblasts synthesize transferrin (Tf). Two types of Tf are present; one is produced by the cells and the other is internalized from the culture medium. To study the metabolism of intracellular Tf, we investigated the subcellular localization of the two types of Tf in human fibroblasts by immunocytochemical and fluorescence-labeling techniques. The internalized Tf was found to be localized in the perinuclear area, and the synthesized Tf was associated with microtubules, forming a fibrous structure in the cytoplasm. When the cells were treated with colchicine which depolymerizes microtubules irreversibly, the synthesized Tf lost its fibrous structure and spread out in cytoplasm, but the internalized Tf remained around the nucleus. These results suggest that the two types of Tf are regulated differently in the cells.
| Original language | English |
|---|---|
| Pages (from-to) | 69-72 |
| Number of pages | 4 |
| Journal | Cell Structure and Function |
| Volume | 23 |
| Issue number | 2 |
| Publication status | Published - Apr 1998 |
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Keywords
- Human fibroblasts
- Microtubules
- Transferrin
ASJC Scopus subject areas
- Physiology
- Structural Biology
- Cell Biology
Cite this
Differential localization of two types of transferrin : Produced by human fibroblasts or incorporated from culture medium. / Sakaguchi, Masakiyo; Kondo, Tadashi; Pu, Hong; Namba, Masayoshi.
In: Cell Structure and Function, Vol. 23, No. 2, 04.1998, p. 69-72.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Differential localization of two types of transferrin
T2 - Produced by human fibroblasts or incorporated from culture medium
AU - Sakaguchi, Masakiyo
AU - Kondo, Tadashi
AU - Pu, Hong
AU - Namba, Masayoshi
PY - 1998/4
Y1 - 1998/4
N2 - In a previous paper, we demonstrated that cultured human fibroblasts synthesize transferrin (Tf). Two types of Tf are present; one is produced by the cells and the other is internalized from the culture medium. To study the metabolism of intracellular Tf, we investigated the subcellular localization of the two types of Tf in human fibroblasts by immunocytochemical and fluorescence-labeling techniques. The internalized Tf was found to be localized in the perinuclear area, and the synthesized Tf was associated with microtubules, forming a fibrous structure in the cytoplasm. When the cells were treated with colchicine which depolymerizes microtubules irreversibly, the synthesized Tf lost its fibrous structure and spread out in cytoplasm, but the internalized Tf remained around the nucleus. These results suggest that the two types of Tf are regulated differently in the cells.
AB - In a previous paper, we demonstrated that cultured human fibroblasts synthesize transferrin (Tf). Two types of Tf are present; one is produced by the cells and the other is internalized from the culture medium. To study the metabolism of intracellular Tf, we investigated the subcellular localization of the two types of Tf in human fibroblasts by immunocytochemical and fluorescence-labeling techniques. The internalized Tf was found to be localized in the perinuclear area, and the synthesized Tf was associated with microtubules, forming a fibrous structure in the cytoplasm. When the cells were treated with colchicine which depolymerizes microtubules irreversibly, the synthesized Tf lost its fibrous structure and spread out in cytoplasm, but the internalized Tf remained around the nucleus. These results suggest that the two types of Tf are regulated differently in the cells.
KW - Human fibroblasts
KW - Microtubules
KW - Transferrin
UR - http://www.scopus.com/inward/record.url?scp=0031857512&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0031857512&partnerID=8YFLogxK
M3 - Article
C2 - 9669034
AN - SCOPUS:0031857512
VL - 23
SP - 69
EP - 72
JO - Cell Structure and Function
JF - Cell Structure and Function
SN - 0386-7196
IS - 2
ER -