Differential localization of two types of transferrin: Produced by human fibroblasts or incorporated from culture medium

Masakiyo Sakaguchi, Tadashi Kondo, Hong Pu, Masayoshi Namba

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

In a previous paper, we demonstrated that cultured human fibroblasts synthesize transferrin (Tf). Two types of Tf are present; one is produced by the cells and the other is internalized from the culture medium. To study the metabolism of intracellular Tf, we investigated the subcellular localization of the two types of Tf in human fibroblasts by immunocytochemical and fluorescence-labeling techniques. The internalized Tf was found to be localized in the perinuclear area, and the synthesized Tf was associated with microtubules, forming a fibrous structure in the cytoplasm. When the cells were treated with colchicine which depolymerizes microtubules irreversibly, the synthesized Tf lost its fibrous structure and spread out in cytoplasm, but the internalized Tf remained around the nucleus. These results suggest that the two types of Tf are regulated differently in the cells.

Original languageEnglish
Pages (from-to)69-72
Number of pages4
JournalCell structure and function
Volume23
Issue number2
DOIs
Publication statusPublished - Apr 1998

Keywords

  • Human fibroblasts
  • Microtubules
  • Transferrin

ASJC Scopus subject areas

  • Physiology
  • Molecular Biology
  • Cell Biology

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