Differential induction of c-Fos and phosphorylated ERK by a noxious stimulus after peripheral nerve injury

Mitsuyasu Tabata, Ryuji Terayama, Kotaro Maruhama, Seiji Iida, Tomosada Sugimoto

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Purpose: In this study, we compared induction of c-Fos and phosphorylated extracellular signal-regulated kinase (p-ERK) in the spinal dorsal horn after peripheral nerve injury. Materials and methods: We examined the spinal dorsal horn for noxious heat-induced c-Fos and p-ERK protein-like immunoreactive (c-Fos- and p-ERK-IR) neuron profiles after tibial nerve injury. The effect of administration of a MEK 1/2 inhibitor (PD98059) on noxious heat-induced c-Fos expression was also examined after tibial nerve injury. Results: A large number of c-Fos- and p-ERK-IR neuron profiles were induced by noxious heat stimulation to the hindpaw in sham-operated animals. A marked reduction in the number of c-Fos- and p-ERK-IR neuron profiles was observed in the medial 1/3 (tibial territory) of the dorsal horn at 3 and 7 days after nerve injury. Although c-Fos-IR neuron profiles had reappeared by 14 days after injury, the number of p-ERK-IR neuron profiles remained decreased in the tibial territory of the superficial dorsal horn. Double immunofluorescence labeling for c-Fos and p-ERK induced by noxious heat stimulation to the hindpaw at different time points revealed that a large number of c-Fos-IR, but not p-ERK-IR, neuron profiles were distributed in the tibial territory after injury. Although administration of a MEK 1/2 inhibitor to the spinal cord suppressed noxious heat-induced c-Fos expression in the peroneal territory, this treatment did not alter c-Fos induction in the tibial territory after nerve injury. Conclusions: ERK phosphorylation may be involved in c-Fos induction in normal nociceptive responses, but not in exaggerated c-Fos induction after nerve injury.

Original languageEnglish
Pages (from-to)208-218
Number of pages11
JournalInternational Journal of Neuroscience
Volume128
Issue number3
DOIs
Publication statusPublished - Mar 4 2018

Keywords

  • ERK phosphorylation
  • c-Fos
  • nerve injury
  • neuropathic pain
  • spinal dorsal horn

ASJC Scopus subject areas

  • Neuroscience(all)

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