Differential induction of c-Fos and phosphorylated ERK by a noxious stimulus after peripheral nerve injury

Mitsuyasu Tabata, Ryuji Terayama, Kotaro Maruhama, Seiji Iida, Tomosada Sugimoto

Research output: Contribution to journalArticle

Abstract

Purpose: In this study, we compared induction of c-Fos and phosphorylated extracellular signal-regulated kinase (p-ERK) in the spinal dorsal horn after peripheral nerve injury. Materials and methods: We examined the spinal dorsal horn for noxious heat-induced c-Fos and p-ERK protein-like immunoreactive (c-Fos- and p-ERK-IR) neuron profiles after tibial nerve injury. The effect of administration of a MEK 1/2 inhibitor (PD98059) on noxious heat-induced c-Fos expression was also examined after tibial nerve injury. Results: A large number of c-Fos- and p-ERK-IR neuron profiles were induced by noxious heat stimulation to the hindpaw in sham-operated animals. A marked reduction in the number of c-Fos- and p-ERK-IR neuron profiles was observed in the medial 1/3 (tibial territory) of the dorsal horn at 3 and 7 days after nerve injury. Although c-Fos-IR neuron profiles had reappeared by 14 days after injury, the number of p-ERK-IR neuron profiles remained decreased in the tibial territory of the superficial dorsal horn. Double immunofluorescence labeling for c-Fos and p-ERK induced by noxious heat stimulation to the hindpaw at different time points revealed that a large number of c-Fos-IR, but not p-ERK-IR, neuron profiles were distributed in the tibial territory after injury. Although administration of a MEK 1/2 inhibitor to the spinal cord suppressed noxious heat-induced c-Fos expression in the peroneal territory, this treatment did not alter c-Fos induction in the tibial territory after nerve injury. Conclusions: ERK phosphorylation may be involved in c-Fos induction in normal nociceptive responses, but not in exaggerated c-Fos induction after nerve injury.

Original languageEnglish
Pages (from-to)208-218
Number of pages11
JournalInternational Journal of Neuroscience
Volume128
Issue number3
DOIs
Publication statusPublished - Mar 4 2018

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Peripheral Nerve Injuries
Extracellular Signal-Regulated MAP Kinases
Neurons
Wounds and Injuries
Hot Temperature
Tibial Nerve
Mitogen-Activated Protein Kinase Kinases
Fluorescent Antibody Technique
Spinal Cord
Phosphorylation
Spinal Cord Dorsal Horn

Keywords

  • c-Fos
  • ERK phosphorylation
  • nerve injury
  • neuropathic pain
  • spinal dorsal horn

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Differential induction of c-Fos and phosphorylated ERK by a noxious stimulus after peripheral nerve injury. / Tabata, Mitsuyasu; Terayama, Ryuji; Maruhama, Kotaro; Iida, Seiji; Sugimoto, Tomosada.

In: International Journal of Neuroscience, Vol. 128, No. 3, 04.03.2018, p. 208-218.

Research output: Contribution to journalArticle

Tabata, Mitsuyasu ; Terayama, Ryuji ; Maruhama, Kotaro ; Iida, Seiji ; Sugimoto, Tomosada. / Differential induction of c-Fos and phosphorylated ERK by a noxious stimulus after peripheral nerve injury. In: International Journal of Neuroscience. 2018 ; Vol. 128, No. 3. pp. 208-218.
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