Differential gene regulation by VIVand VV ions in the branchial sac, intestine, and blood cells of a vanadium-rich ascidian, Ciona intestinalis

Satoshi Kume, Tatsuya Ueki, Hiroki Matsuoka, Mayuko Hamada, Nori Satoh, Hitoshi Michibata

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Ascidians are hyperaccumulators that have been studied in detail. Proteins and genes involved in the accumulation process have been identified, but regulation of gene expression related to vanadium accumulation remains unknown. To gain insights into the regulation of gene expression by vanadium in a genome-wide manner, we performed a comprehensive study on the effect of excess vanadium ions on a vanadium-rich ascidian, Ciona intestinalis, using a microarray. RT-PCR and enzyme activity assay were performed from the perspective of redox and accumulation of metal ions in each tissue. Glutathione metabolism- related proteins were significantly up-regulated by VIV treatment. Several genes involved in the transport of vanadium and protons, such as Nramp and V-ATPase, were significantly up-regulated by VIV treatment. We observed significant up-regulation of glutathione synthesis and degradation pathways in the intestine and branchial sac. In blood cells, expression of Ci-Vanabin4, glutathione reductase activity, glutathione levels, and vanadium concentration increased after VIV treatment. VIV treatment induced significant changes related to vanadium exclusion, seclusion, and redox pathways in the intestine and branchial sac. It also induced an enhancement of the vanadium reduction and accumulation cascade in blood cells. These differential responses in each tissue in the presence of excess vanadium ions suggest that vanadium accumulation and reduction may have regulatory functions. This is the first report on the gene regulation by the treatment of vanadium-rich ascidians with excess vanadium ions. It provided much information for the mechanism of regulation of gene expression related to vanadium accumulation.

Original languageEnglish
Pages (from-to)1037-1050
Number of pages14
JournalBiometals
Volume25
Issue number5
DOIs
Publication statusPublished - Oct 1 2012
Externally publishedYes

Keywords

  • Ascidian
  • Gene regulation
  • Glutathione
  • Microarray
  • Vanadium

ASJC Scopus subject areas

  • Biomaterials
  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • Metals and Alloys

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