Differential expression of protease M/neurosin in oligodendrocytes and their progenitors in an animal model of multiple sclerosis

Ryuji Terayama, Yoshio Bando, Ying Ping Jiang, Branka Mitrovic, Shigetaka Yoshida

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

To determine the possible involvement of protease M/neurosin in demyelinating diseases of the CNS, we examined its expression in myelin oligodendrocyte glycoprotein (MOG)-induced experimental autoimmune encephalomyelitis (EAE), a recognized animal model of multiple sclerosis (MS). In situ hybridization, immunohistochemistry and quantitative real-time polymerase chain reaction (PCR) demonstrated that EAE caused an increase in the expression of protease M/neurosin mRNA and its protein product throughout the white and gray matter surrounding demyelinating lesions. Combined in situ hybridization and immunohistochemistry demonstrated that most of the cells expressing protease M/neurosin mRNA within control spinal cord showed immunoreactivity for CNPase or NG2, cell-specific markers for oligodendrocytes and their progenitors, respectively. In the spinal cord from mice with EAE, the expression of protease M/neurosin mRNA in CNPase-positive cells appeared to be increased while double-labeled cells positive for protease M/neurosin mRNA and NG2 were rarely found in areas associated with demyelinating lesions. Although a prominent accumulation of inflammatory cells including T-cells was observed in the vicinity of demyelinated lesions, these cells were not associated with protease M/neurosin mRNA expression. The levels of protease M/neurosin mRNA expression were unchanged in the spleen and even decreased in the thymus during the course of EAE. These observations suggest that the differential expression of protease M/neurosin in mature oligodendrocytes and their progenitors is involved in the pathogenesis of MS and EAE.

Original languageEnglish
Pages (from-to)82-87
Number of pages6
JournalNeuroscience Letters
Volume382
Issue number1-2
DOIs
Publication statusPublished - Jul 1 2005
Externally publishedYes

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Oligodendroglia
Multiple Sclerosis
Animal Models
Autoimmune Experimental Encephalomyelitis
Messenger RNA
2',3'-Cyclic-Nucleotide Phosphodiesterases
In Situ Hybridization
Spinal Cord
Immunohistochemistry
Myelin-Oligodendrocyte Glycoprotein
M-protease
Demyelinating Diseases
Thymus Gland
Real-Time Polymerase Chain Reaction
Spleen
T-Lymphocytes

Keywords

  • Experimental autoimmune encephalomyelitis
  • Multiple sclerosis
  • Oligodendrocytes
  • Serine protease

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Differential expression of protease M/neurosin in oligodendrocytes and their progenitors in an animal model of multiple sclerosis. / Terayama, Ryuji; Bando, Yoshio; Jiang, Ying Ping; Mitrovic, Branka; Yoshida, Shigetaka.

In: Neuroscience Letters, Vol. 382, No. 1-2, 01.07.2005, p. 82-87.

Research output: Contribution to journalArticle

Terayama, Ryuji ; Bando, Yoshio ; Jiang, Ying Ping ; Mitrovic, Branka ; Yoshida, Shigetaka. / Differential expression of protease M/neurosin in oligodendrocytes and their progenitors in an animal model of multiple sclerosis. In: Neuroscience Letters. 2005 ; Vol. 382, No. 1-2. pp. 82-87.
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