Differential expression of macrophage inflammatory protein-2 and monocyte chemoattractant protein-1 in experimental glomerulonephritis

Frederick W K Tam, Ayman M. Karkar, Jennifer Smith, Teizo Yoshimura, Alexander Steinkasserer, Roland Kurrle, Klaus Langner, Andrew J. Rees

Research output: Contribution to journalArticle

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Abstract

We examined the relation between glomerular expression of chemokines from α-subfamily (macrophage inflammatory protein-2, MIP-2) and β-subfamily (monocyte chemoattractant protein-1, MCP-1) and infiltration of neutrophils and monocytes in antibody mediated glomerulonephritis in rats. In the accelerated model of nephrotoxic nephritis (NTN), glomerular expression of MIP-2 and MCP-1 genes correlated with the sequential migration of neutrophil and monocyte; influx, respectively. These relationships were investigated further in the heterologous phase of NTN by applying various treatments known to modulate the severity of injury. Pretreatment with bacterial lipopolysaccharide resulted in greater injury, MIP-2 expression increased 25- to 50-fold, and the glomerular neutrophil count increased two- to fourfold. Both MIP-2 mRNA levels and neutrophil infiltration were reduced by additional pretreatment with IL-6, IL-1 receptor antagonist, soluble IL-1 receptor or soluble TNF receptor (Spearman correlation coefficient r = 0.897, P <0.005). In the heterologous phase of NTN, different pre-treatments only resulted in trivial changes in MCP-1 expression and monocyte infiltration. In conclusion, glomerular MIP-2 gene expression correlates with neutrophil infiltration both temporally during the evolution of nephritis, and when glomerular injury is modified by treatment. Glomerular MCP-1 gene expression correlates with monocyte influx. The data show chemokines of α- and β-subfamilies co-operative to cause selective and sequential migration of different leukocyte subsets during development of antibody mediated glomerulonephritis.

Original languageEnglish
Pages (from-to)715-721
Number of pages7
JournalKidney International
Volume49
Issue number3
Publication statusPublished - 1996
Externally publishedYes

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Chemokine CXCL2
Chemokine CCL2
Glomerulonephritis
Nephritis
Neutrophil Infiltration
Monocytes
Interleukin-1 Receptors
Chemokines
Wounds and Injuries
Neutrophils
Gene Expression
Antibodies
Tumor Necrosis Factor Receptors
Lipopolysaccharides
Interleukin-6
Leukocytes
Messenger RNA
Genes

ASJC Scopus subject areas

  • Nephrology

Cite this

Tam, F. W. K., Karkar, A. M., Smith, J., Yoshimura, T., Steinkasserer, A., Kurrle, R., ... Rees, A. J. (1996). Differential expression of macrophage inflammatory protein-2 and monocyte chemoattractant protein-1 in experimental glomerulonephritis. Kidney International, 49(3), 715-721.

Differential expression of macrophage inflammatory protein-2 and monocyte chemoattractant protein-1 in experimental glomerulonephritis. / Tam, Frederick W K; Karkar, Ayman M.; Smith, Jennifer; Yoshimura, Teizo; Steinkasserer, Alexander; Kurrle, Roland; Langner, Klaus; Rees, Andrew J.

In: Kidney International, Vol. 49, No. 3, 1996, p. 715-721.

Research output: Contribution to journalArticle

Tam, FWK, Karkar, AM, Smith, J, Yoshimura, T, Steinkasserer, A, Kurrle, R, Langner, K & Rees, AJ 1996, 'Differential expression of macrophage inflammatory protein-2 and monocyte chemoattractant protein-1 in experimental glomerulonephritis', Kidney International, vol. 49, no. 3, pp. 715-721.
Tam, Frederick W K ; Karkar, Ayman M. ; Smith, Jennifer ; Yoshimura, Teizo ; Steinkasserer, Alexander ; Kurrle, Roland ; Langner, Klaus ; Rees, Andrew J. / Differential expression of macrophage inflammatory protein-2 and monocyte chemoattractant protein-1 in experimental glomerulonephritis. In: Kidney International. 1996 ; Vol. 49, No. 3. pp. 715-721.
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