Differential expression of heat shock protein 70 gene between the cortex and caudate after transient focal cerebral ischaemia in rats

Koji Abe, J. Kawagoe, T. Araki, M. Aoki, K. Kogure

Research output: Contribution to journalArticle

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Abstract

In relation to changes of total protein synthesis, induction of 70-kDa heat shock protein (HSP70) mRNA was examined by Northern blot and in situ hybridization after 30 min of transient middle cerebral artery (MCA) occlusion of rats. HSP70 mRNA was not present in the control condition of brain. With reperfusion, the mRNA was greatly induced along with the recovery of total protein synthesis in the cerebral cortex and lateral caudate of the ipsilateral hemisphere. However, the level of the mRNA reached a maximum earlier in the lateral caudate (at 3 h) than in the cortex (at 8 h), and the maximum amount of the mRNA was much smaller in the caudate than in the cortex. Total protein synthesis in the lateral caudate did not completely recover until 7 days. Histological examination showed a severe damage in cells of lateral caudate, while cells in the cortex were almost normal at 7 days. No difference in the brain temperature was observed between the two regions. These results show that the induction of HSP70 mRNA correlates with the recovery of protein synthesis in brain cells after a transient ischaemia, and that the HSP70 gene expression is different at the transcriptional level between the cortical and caudate cells after the transient ischaemia.

Original languageEnglish
Pages (from-to)381-385
Number of pages5
JournalNeurological Research
Volume14
Issue number5
Publication statusPublished - 1992
Externally publishedYes

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HSP70 Heat-Shock Proteins
Transient Ischemic Attack
Messenger RNA
Genes
Brain
Proteins
Ischemia
Middle Cerebral Artery Infarction
Northern Blotting
Cerebral Cortex
Reperfusion
In Situ Hybridization
Gene Expression
Temperature

ASJC Scopus subject areas

  • Clinical Neurology
  • Neuroscience(all)

Cite this

Differential expression of heat shock protein 70 gene between the cortex and caudate after transient focal cerebral ischaemia in rats. / Abe, Koji; Kawagoe, J.; Araki, T.; Aoki, M.; Kogure, K.

In: Neurological Research, Vol. 14, No. 5, 1992, p. 381-385.

Research output: Contribution to journalArticle

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AU - Kawagoe, J.

AU - Araki, T.

AU - Aoki, M.

AU - Kogure, K.

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AB - In relation to changes of total protein synthesis, induction of 70-kDa heat shock protein (HSP70) mRNA was examined by Northern blot and in situ hybridization after 30 min of transient middle cerebral artery (MCA) occlusion of rats. HSP70 mRNA was not present in the control condition of brain. With reperfusion, the mRNA was greatly induced along with the recovery of total protein synthesis in the cerebral cortex and lateral caudate of the ipsilateral hemisphere. However, the level of the mRNA reached a maximum earlier in the lateral caudate (at 3 h) than in the cortex (at 8 h), and the maximum amount of the mRNA was much smaller in the caudate than in the cortex. Total protein synthesis in the lateral caudate did not completely recover until 7 days. Histological examination showed a severe damage in cells of lateral caudate, while cells in the cortex were almost normal at 7 days. No difference in the brain temperature was observed between the two regions. These results show that the induction of HSP70 mRNA correlates with the recovery of protein synthesis in brain cells after a transient ischaemia, and that the HSP70 gene expression is different at the transcriptional level between the cortical and caudate cells after the transient ischaemia.

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